Bayesian estimation of Tritrichomonas foetus diagnostic test sensitivity and specificity in range beef bulls

Pérez, Andrés; Cobo, Eduardo R.; Martínez, Alfredo; Campero, Carlos Manuel; Späth, Ernesto

doi:10.1016/j.vetpar.2006.06.021

Cited by 17 publications

(20 citation statements)

References 15 publications

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“…Of 29 samples collected from five laboratory-infected bulls with a resting period of 2-4 days, 24 (83 %) were positive (Mukhufhi et al, 2003). In a field sampling of 2832 mature bulls from 124 beef herds in Salado River basin, Buenos Aires, Argentina, the sensitivity and specificity by Bayesian estimation were 72.0 % (59-87 %) and 95.4 % (94-96 %), respectively (Perez et al, 2006). Among 143 samples collected from 13 naturally infected bulls, 139 (97 %) were positive by culture for up to 4 days.…”

Section: Culturementioning

confidence: 99%

Diagnosis of Tritrichomonas foetus-infected bulls, an ultimate approach to eradicate bovine trichomoniasis in US cattle?

Yao

2013

Journal of Medical Microbiology

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Diagnosis ofBovine trichomoniasis is a sexually transmitted protozoan disease with a worldwide distribution. It has been endemic in the USA for more than 80 years. Mississippi and all the states west of the Mississippi River, except Iowa and Minnesota, have rules/regulations to reduce the spread of the disease. The core of these regulations consists of testing bulls and prohibiting importation of nonTritrichomonas foetus-free bulls. Factors such as sampling methods and intervals, shipping medium and temperature, and testing techniques are reviewed for their effect on diagnostic accuracy. Finally, a comprehensive approach for controlling and eventually eradicating the disease is presented.

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Section: Culturementioning

confidence: 99%

Diagnosis of Tritrichomonas foetus-infected bulls, an ultimate approach to eradicate bovine trichomoniasis in US cattle?

Yao

2013

Journal of Medical Microbiology

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“…With the reported high analytical sensitivity of PCR, a move to using PCR as a stand-alone test has been seen with many state regulations relying on PCR in lieu of culture [10][11][12][13][14]. However, PCR is costly and may result in false positives.…”

Section: Pcr and Qpcrmentioning

confidence: 99%

Tritrichomonas foetus in Beef Bulls

2014

J Veterinar Sci Technol

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Tritrichomonas foetus is a production and regulatory concern for beef producers in the Western United States and more recently in states of the Mississippi Valley. Traditionally preputial scrapings have been collected, cultured in enriched media, and examined microscopically. PCR techniques are now being used extensively to confirm culture results or as a stand-alone test for the organism. This technology offers the advantage of distinguishing the pathogenic Tritrichomonas foetus organism from other nonpathogenic fecal organisms.

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“…A considerable variation of reported culture sensitivity (Se) exists ranging from as low as 70% to as high as 92%. 5,8,12 However, a potential pitfall of culture alone is the lack of specificity (Sp), and false positive results may lead to the sacrifice of valuable breeding animals at a considerable expense to the producer. 5 In order to avoid undue sacrifice, follow-up polymerase chain reaction (PCR) testing on culturepositive samples has been recommended.…”

mentioning

confidence: 99%

“…2,3,6,10 PCR for T. foetus has a reported Se of 98.38% and a Sp of 93.75%, relative to culture. 9,12 Sensitivity for culture alone has been reported as low as 70% for a single culture and as high as 97.3% for 3 successive cultures completed 1 week apart. 9,12 Because of its high reported Se some state T. foetus control programs have forgone culture and moved directly to PCR, either as a single stand-alone test or using repeated tests on samples collected within 7 to 10 days of each other.…”

mentioning

confidence: 99%

“…9,12 Sensitivity for culture alone has been reported as low as 70% for a single culture and as high as 97.3% for 3 successive cultures completed 1 week apart. 9,12 Because of its high reported Se some state T. foetus control programs have forgone culture and moved directly to PCR, either as a single stand-alone test or using repeated tests on samples collected within 7 to 10 days of each other. 8,11 Tritrichomonas foetus PCR is potentially the test of choice for the detection of T. foetus but is limited by the cost of individual laboratory tests, ranging from $20.00 to $35.00 plus veterinarian charges for sample collection.…”

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confidence: 99%

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Pooled Polymerase Chain Reaction to DetectTritrichomonas Foetusin Beef Bulls

et al. 2008

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Abstract. Preputial scraping samples from 305 mixed breed beef bulls were examined for the detection of Tritrichomonas foetus infection. All samples were collected by veterinarians and transported in commercial media to an accredited lab. Upon arrival samples underwent microscopic examination for the presence of Tritrichomonas foetus and were then incubated until 5 days postcollection before final microscopic examination. Culture detected 14 samples with Trichomonad spp.; all were confirmed to be Tritrichomonas foetus by polymerase chain reaction (PCR). After final examination samples were randomly placed in groups of 5 samples; technicians were blinded as to culture results of the individual samples constituting each pool. From each sample within a group, a portion of the fluid sediment was removed and pooled with the other samples of the group to form 61 pools. From each of the formed pools an aliquot was removed for PCR. PCR detected 16 positive pools; an additional 2 positive samples were then identified on individual PCR on samples previously diagnosed as culture negative. Relative to culture, the 95% confidence intervals for sensitivity and specificity of PCR pools to detect Tritrichomonas foetus were 76.8% to 100% (mean value: 100%) and 85.5 to 99.5% (mean value: 93.4%), respectively.

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Bayesian estimation of Tritrichomonas foetus diagnostic test sensitivity and specificity in range beef bulls

Cited by 17 publications

References 15 publications

Diagnosis of Tritrichomonas foetus-infected bulls, an ultimate approach to eradicate bovine trichomoniasis in US cattle?

Diagnosis of Tritrichomonas foetus-infected bulls, an ultimate approach to eradicate bovine trichomoniasis in US cattle?

Tritrichomonas foetus in Beef Bulls

Pooled Polymerase Chain Reaction to DetectTritrichomonas Foetusin Beef Bulls

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