Bats with hATs: Evidence for Recent DNA Transposon Activity in Genus Myotis

Ray, David A.; Pagán, Heidi J. T.; Thompson, Michelle L.; Stevens, Richard D.

doi:10.1093/molbev/msl192

Cited by 78 publications

(86 citation statements)

References 32 publications

(27 reference statements)

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“…The Buster transposons in contrast have the TSD consensus of 59-nnnTAnnn-39, which we derived both from sequences flanking Buster transposons in their native genomes and from the transposition assays described below. This TSD was also reported flanking hAT sequences in M. lucifugus (Ray et al 2006), which we now classify as being members of the Buster family. It remains to be determined which part of the hAT transposase mediates target-site recognition.…”

Section: Resultssupporting

confidence: 78%

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Phylogenetic and Functional Characterization of the hAT Transposon Superfamily

et al. 2011

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Transposons are found in virtually all organisms and play fundamental roles in genome evolution. They can also acquire new functions in the host organism and some have been developed as incisive genetic tools for transformation and mutagenesis. The hAT transposon superfamily contains members from the plant and animal kingdoms, some of which are active when introduced into new host organisms. We have identified two new active hAT transposons, AeBuster1, from the mosquito Aedes aegypti and TcBuster from the red flour beetle Tribolium castaneum. Activity of both transposons is illustrated by excision and transposition assays performed in Drosophila melanogaster and Ae. aegypti and by in vitro strand transfer assays. These two active insect transposons are more closely related to the Buster sequences identified in humans than they are to the previously identified active hAT transposons, Ac, Tam3, Tol2, hobo, and Hermes. We therefore reexamined the structural and functional relationships of hAT and hAT-like transposase sequences extracted from genome databases and found that the hAT superfamily is divided into at least two families. This division is supported by a difference in target-site selections generated by active transposons of each family. We name these families the Ac and Buster families after the first identified transposon or transposon-like sequence in each. We find that the recently discovered SPIN transposons of mammals are located within the family of Buster elements.

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Section: Resultssupporting

confidence: 78%

“…The Buster family contains the recently described transposons from the bat, M. lucifgus (Ray et al 2006). Myotis hAT forms a clade with Buster transposase sequences from another bat transposase (MlBuster1), a mosquito (Ae.…”

Section: Resultsmentioning

confidence: 99%

Phylogenetic and Functional Characterization of the hAT Transposon Superfamily

et al. 2011

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“…In particular, the genome is packed with Helitrons (at least 3% of the genome) (158), whereas none are recognizable in any of 22 other placental species (including two other bat species) for which a substantial amount of genomic sequences is now available. In contrast to other mammals so far examined, the recent data point to a continuous colonization of the vesper bat genome(s) by various DNA transposon families (158,161; D. Ray. J. Smith, H.J.T.…”

Section: Differential Success Of Dna Transposons Among Speciesmentioning

confidence: 94%

DNA Transposons and the Evolution of Eukaryotic Genomes

2007

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Transposable elements are mobile genetic units that exhibit broad diversity in their structure and transposition mechanisms. Transposable elements occupy a large fraction of many eukaryotic genomes and their movement and accumulation represent a major force shaping the genes and genomes of almost all organisms. This review focuses on DNA-mediated or class 2 transposons and emphasizes how this class of elements is distinguished from other types of mobile elements in terms of their structure, amplification dynamics, and genomic effect. We provide an up-to-date outlook on the diversity and taxonomic distribution of all major types of DNA transposons in eukaryotes, including Helitrons and Mavericks. We discuss some of the evolutionary forces that influence their maintenance and diversification in various genomic environments. Finally, we highlight how the distinctive biological features of DNA transposons have contributed to shape genome architecture and led to the emergence of genetic innovations in different eukaryotic lineages.

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“…h AT elements comprise a large and prevalent group of Class II transposable elements found in a wide range of plants and animals (Kempken and Windhofer 2001;Kunze and Weil 2002;Ray et al 2007). hAT elements are not only of interest for their role in genome evolution but also as tools for genetically modifying organisms, with the elements Hermes and hobo being two examples of hAT element-derived insect gene vectors (Blackman et al 1989;O'Brochta et al 1996).…”

mentioning

confidence: 99%

Transposable Element Dynamics of the hAT Element Herves in the Human Malaria Vector Anopheles gambiae s.s.

et al. 2007

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Transposable elements are being considered as genetic drive agents for introducing phenotype-altering genes into populations of vectors of human disease. The dynamics of endogenous elements will assist in predicting the behavior of introduced elements. Transposable element display was used to estimate the siteoccupancy frequency distribution of Herves in six populations of Anopheles gambiae s.s. The site-occupancy distribution data suggest that the element has been recently active within the sampled populations. All 218 individuals sampled contained at least one copy of Herves with a mean of 3.6 elements per diploid genome. No significant differences in copy number were observed among populations. Nucleotide polymorphism within the element was high (p ¼ 0.0079 in noncoding sequences and 0.0046 in coding sequences) relative to that observed in some of the more well-studied elements in Drosophila melanogaster. In total, 33 distinct forms of Herves were found on the basis of the sequence of the first 528 bp of the transposase open reading frame. Only two forms were found in all six study populations. Although Herves elements in An. gambiae are quite diverse, 85% of the individuals examined had evidence of complete forms of the element. Evidence was found for the lateral transfer of Herves from an unknown source into the An. gambiae lineage prior to the diversification of the An. gambiae species complex. The characteristics of Herves in An. gambiae are somewhat unlike those of P elements in D. melanogaster.

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Bats with hATs: Evidence for Recent DNA Transposon Activity in Genus Myotis

Cited by 78 publications

References 32 publications

Phylogenetic and Functional Characterization of the hAT Transposon Superfamily

Phylogenetic and Functional Characterization of the hAT Transposon Superfamily

DNA Transposons and the Evolution of Eukaryotic Genomes

Transposable Element Dynamics of the hAT Element Herves in the Human Malaria Vector Anopheles gambiae s.s.

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