DNA Transposons and the Evolution of Eukaryotic Genomes

Feschotte, Cleacuteldric; Pritham, Ellen J.

doi:10.1146/annurev.genet.40.110405.090448

Cited by 999 publications

(1,012 citation statements)

References 245 publications

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“…The MITEs are, in general, derived from ancient, related autonomous elements, and their origin can occur through internal deletions in autonomous elements, where the only remaining are the terminal inverted repeats (TIRs) and, sometimes, portions between the TIRs and the coding region of the transposase. This origin supports the proposal of their mobilization in trans by a transposase encoded by a full-length element (Feschotte & Pritham, 2007). The autonomous transposons use the cellular machinery of the host cells for the protein synthesis necessary for their mobilization, whereas the MITEs use the machinery encoded by transposons for mobilization.…”

Section: Introductionsupporting

confidence: 83%

msechBari, a new MITE-like element inDrosophila sechelliarelated to theBaritransposon

Dias

Carareto

2011

Genet. Res.

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SummaryA few occurrences of miniature inverted-repeat transposable elements (MITEs) have been reported in species of the genus Drosophila. Here, we describe msechBari, a MITE-like element in Drosophila sechellia. The element is short, approximately 90 bp in length, AT-rich and occurs in association with, or close to, genes, characteristics that are typical for MITEs. The identification was performed in silico using the sequenced genome of D. sechellia and confirmed in a laboratory strain. This short element is related to the Bari_DM transposon of Drosophila melanogaster, having terminal inverted repeats (TIRs) of a similar length and a high identity with the full-length Bari_DM element. The estimated recent origin of the element and the homogeneity observed between copies found in the genome suggests that msechBari could be active in D. sechellia.

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Section: Introductionsupporting

confidence: 83%

msechBari, a new MITE-like element inDrosophila sechelliarelated to theBaritransposon

Dias

Carareto

2011

Genet. Res.

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“…23 The fate of TEs in genomes is dictated by a delicate balance between opposite evolutionary forces: On one hand, they can be maintained by genetic drift, high transposition rate or because they provide some advantageous character. On the other hand, they can be purged by stochastic loss, their genetic burden (by selection) or self inactivation, to name a few [47].…”

Section: Co-opted Transposable Elements and (Non)evolution Of Gene Rementioning

confidence: 99%

Control of vitellogenin genes expression by sequences derived from transposable elements in rainbow trout

Bouter

Buisine

Grand

et al. 2010

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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In most of oviparous animals, vitellogenins (VTG) are the major egg yolk precursors. They are produced in the liver under the control of estrogens. In rainbow trout (Oncorhynchus mykiss), the vtg genes cluster contains an unusually large number of almost identical gene copies. In order to identify the regulatory elements in their promoters, we used a combination of reporter plasmids containing genomic sequences including putative estrogen response elements (EREs) and we performed transient transfection assays in MCF-7 and yeast cells.We found a functional ERE corresponding to the sequence GGGGCAnnnTAACCT (rtvtgERE), which differs from the consensus ERE (ERE cs ) by three base pairs. This nonpalindromic ERE is located in the env gene of a retrotransposon relic, 180 base pairs upstream of the transcriptional start site. Fluorescence anisotropy experiments confirmed that the purified human estrogen receptor α (hERα) can specifically bind to rtvtgERE. Furthermore, we observe that the stability of hERα-ERE cs and hERα-rtvtgERE complexes is similar with equilibrium dissociation constants of 3.0 nM and 6.2nM respectively, under our experimental conditions. Additionally, this rtvtgERE sequence displays a high E2-responsiveness through ER activation in cellulo.In the rainbow trout, the functional ERE (rtvtgERE) lies within promoter sequences which are mostly composed of sequences derived from transposable elements (TEs), which therefore may have acted as an evolutionary buffer to secure the proper expression of these genes.4

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“…Similar to all other TEs, DNA transposons are transmitted vertically from parent to offspring; however, their horizontal transmission between species (sometimes phylogenetically distant) is not uncommon. Unlike other TEs, DNA transposons are found in both eukaryotes and prokaryotes (for review see Feschotte and Pritham, 2007).…”

Section: Introductionmentioning

confidence: 99%