Batch Fabrication of High‐Performance Planar Patch‐Clamp Devices in Quartz

Nagarah, John M.; Paek, Eunsu; Luo, Yi; Wang, Pin; Hwang, Gyeong S.; Heath, James R.

doi:10.1002/adma.201001793

Cited by 18 publications

(20 citation statements)

References 40 publications

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“…When a cell covers the electrode surrounded with a non-functionalized ring (Fig. 6B), the seal resistance ranges from 50–300 MΩ (Ν = 6), a finding consistent with the recessed electrode seal resistances found by others2021. When the cell covers an electrode with a functionalized gold ring (Fig.…”

Section: Resultssupporting

confidence: 81%

Biomimetic surface patterning for long-term transmembrane access

VanDersarl

Renaud

2016

Sci Rep

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Here we present a planar patch clamp chip based on biomimetic cell membrane fusion. This architecture uses nanometer length-scale surface patterning to replicate the structure and function of membrane proteins, creating a gigaohm seal between the cell and a planar electrode array. The seal is generated passively during cell spreading, without the application of a vacuum to the cell surface. This interface can enable cell-attached and whole-cell recordings that are stable to 72 hours, and generates no visible damage to the cell. The electrodes can be very small (<5 μm) and closely packed, offering a high density platform for cellular measurement.

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Section: Resultssupporting

confidence: 81%

Biomimetic surface patterning for long-term transmembrane access

VanDersarl

Renaud

2016

Sci Rep

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“…The performance of a planar chip is highly dependent on the material used. Quartz and glass [14], [19] have excellent insulation, hydrophilic, and mechanical properties over the silicon material [15], [16], [20]- [23], thus are more suitable for fabrication.…”

Section: ) Planar Chip Fabricationmentioning

confidence: 99%

Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study

Zhang

Liu

et al. 2014

IEEE/ASME Trans. Mechatron.

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“…However, the traditional PC technology is a really timeconsuming and laborious effort with rather low throughput. For the past decade, various types of PC microchips have been developed in several groups to enhance the throughput and efficiency over the conventional glasspipette based PC technology (Li 2006;Ionescu-Zanetti et al 2005;Penmetsa et al 2010;Nagarah et al 2010;Matthews and Judy 2006). It has been demonstrated that by using properly designed and fabricated micropipette, some PC microchips show reliable and consistent Gigaohms (GΩ) seal capability, indicating the possibility that the PC microchip can eventually replace the conventional glass-pipette PC technology.…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of cell viability using direct electrical measurement with a patch-clamp microchip

Pathak

Zhao

Gong

et al. 2011

Biomed Microdevices

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Real-time tagless monitoring of cell viability using patch-clamp microchips is reported and validated by using fluorescence imaging techniques for the first time. Specifically, four human breast cancer cell lines (MDA-MB231, MDA-MB231-brain metastatic subline (abbreviated as MB231-BR), MB231-BR over-expressing HER2 gene (MB231-BR-HER2), and MB231-BR-vector control for the HER2 (MB231-BR-vector)) have been used for these studies. Systematic experiments on these cells found that the seal impedance/resistance of cells captured by the micro-pipettes always decreases during the process when the cell loses its viability, and therefore it is a valid indicator of live or dead cells. Systematic experiments also found that the Mega-seal of patch-clamp microchip is sufficient for monitoring cell viability. Given its simplicity of direct electrical measurement of cells without fluorescence labeling, this technology may provide an efficient technical platform to monitor the drug effects on cells, thereby significantly benefiting high throughput drug screening and discovery process.

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Batch Fabrication of High‐Performance Planar Patch‐Clamp Devices in Quartz

Cited by 18 publications

References 40 publications

Biomimetic surface patterning for long-term transmembrane access

Biomimetic surface patterning for long-term transmembrane access

Development of Mechanostimulated Patch-Clamp System for Cellular Physiological Study

Real-time monitoring of cell viability using direct electrical measurement with a patch-clamp microchip

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