Batch Biodegradation of Phenol of Paper and Pulp Effluent by Aspergillus Niger

Background: The fungus Aspergillus niger is an important industrial organism for citric acid fermentation; one of the most efficient biotechnological processes. Previously we introduced a dynamic model that captures this process in the industrially relevant batch fermentation setting, providing a more accurate predictive platform to guide targeted engineering. In this article we exploit this dynamic modelling framework, coupled with a robust genetic algorithm for the in silico evolution of A. niger organic acid production, to provide solutions to complex evolutionary goals involving a multiplicity of targets and beyond the reach of simple Boolean gene deletions. We base this work on the latest metabolic models of the parent citric acid producing strain ATCC1015 dedicated to organic acid production with the required exhaustive genomic coverage needed to perform exploratory in silico evolution. Results: With the use of our informed evolutionary framework, we demonstrate targeted changes that induce a complete switch of acid output from citric to numerous different commercially valuable target organic acids including succinic acid. We highlight the key changes in flux patterns that occur in each case, suggesting potentially valuable targets for engineering. We also show that optimum acid productivity is achieved through a balance of organic acid and biomass production, requiring finely tuned flux constraints that give a growth rate optimal for productivity. Conclusions: This study shows how a genome-scale metabolic model can be integrated with dynamic modelling and metaheuristic algorithms to provide solutions to complex metabolic engineering goals of industrial importance. This framework for in silico guided engineering, based on the dynamic batch growth relevant to industrial processes, offers considerable potential for future endeavours focused on the engineering of organisms to produce valuable products.

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“…phenol as a carbon source [26] and phytate as a phosphate source [27]; while the rest are hypothetical.…”

Section: New Substrates In Idu1756mentioning

confidence: 99%

In silico evolution of Aspergillus niger organic acid production suggests strategies for switching acid output

Upton

McQueen‐Mason

Wood

2020

Biotechnol Biofuels

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Co-substrate-mediated utilization of high concentration of phenol by Aspergillus niger FP7 and reduction of its phytotoxicity on Vigna radiata L.

Bhattacharya

Das

Krishnan

et al. 2021

Environ Sci Pollut Res

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The Effect of Some Sugars on the Growth of Aspergillus niger

et al. 2015

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Abstract:The majority of black Aspergilli, including Aspergillus niger. Here, we provide to evidence that the exploration of sugar uptake in the filamentous fungus A. niger as a sole carbon source. The goal of this research line is determination of the growth of fungi was evaluated every 24h, measuring the colony diameter (cm). A. niger was inoculated onto two culture media: PDA: for maintains a strain as pure while Czapeck Dox Agar was used in investigation into their carbon requirement, using five different carbon sources (vizs. glucose, fructose, sucrose, maltose, and starch). The fungus was tested grew sparsely on the basal medium lacking in carbon, which was the control. However this fungus was found to vary from their ability to use the supplied sources of carbon. Fructose and sucrose were found to be suitable sources of carbon for a fungal isolates, whereas glucose and maltose proved good carbon source to have a higher affinity. Starch as a polysaccharide, was a poor source of carbon for the growth of this isolate. Despite earlier claims, saccharides rather than monosaccharide were breakdowns extracellularly by means of a broad range of extracellular enzyme activities from Aspergillus niger.

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Batch Biodegradation of Phenol of Paper and Pulp Effluent by Aspergillus Niger

Cited by 3 publications

References 18 publications

In silico evolution of Aspergillus niger organic acid production suggests strategies for switching acid output

In silico evolution of Aspergillus niger organic acid production suggests strategies for switching acid output

Co-substrate-mediated utilization of high concentration of phenol by Aspergillus niger FP7 and reduction of its phytotoxicity on Vigna radiata L.

The Effect of Some Sugars on the Growth of Aspergillus niger

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