Batch and dynamic adsorption of lysozyme from chicken egg white on dye-affinity nanofiber membranes modified by ethylene diamine and chitosan

Show, Pau Loke; Ooi, Chien Wei; Lee, Xin Jiat; Yang, Chi; Liu, Bing Lan; Chang, Yu-Kaung

doi:10.1016/j.ijbiomac.2020.08.065

Cited by 32 publications

(37 citation statements)

References 48 publications

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“…Chitosan nanofibers have diameter around 10 nm and amino groups on its surface with positive charge [98]. Accordingly, chitosan nanofibers possess unique characteristics and advantages that other nanofiber does not have, that has been expected to be used in various sorts of industrial fields, for instance, filtrations, recovery of metal ions, adsorption of proteins, drug release, enzyme carriers, wound healing, cosmetics, and biosensors have been developed [99][100][101][102][103][104][105][106].…”

Section: Chitosan Nanofibermentioning

confidence: 99%

Innovative Separation Technology Utilizing Marine Bioresources: Multifaceted Development of a Chitosan-Based System Leading to Environmentally-Friendly Processes

Kashima¹,

Takahashi²,

Nakayama³

et al. 2021

Chitin and Chitosan - Physicochemical Properties and Industrial Applications [Working Title]

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Chitosan, known as a most typical marine biological polymer, has a fruitful capability of biocompatible gel formation. Attempts of chitosan have been made to develop it from the multifaceted viewpoint of separation technology. The physicochemical properties of chitosan containing a lot of hydroxyl groups and reactive amino groups help to build the characteristic polymer networks. The deacetylation degree of chitosan is found as the most influential factor to regulate properties of chitosan hydrogels. The antibacterial activity of the chitosan membrane is one of its notable abilities because of its practical application. The chitosan, its derivatives, and the complex formation with other substances has been used for applications in filtration and membrane separation processes. Adsorption processes based on chitosan have been also developed widely. Moreover, complex of chitosan gel helps to immobilize adsorbent particles. The chitosan membrane immobilizing Prussian-Blue for cesium ion removal from the aqueous phase is one of the leading cases. To elaborate the adsorption behavior on the chitosan immobilizing adsorbent, the isothermal equilibrium and mass transfer characteristics can be discussed. The adsorption process using chitosan-based membranes in combination with filtration in a flow process is advantageous compared with the batch process. More advanced studies of chitosan aerogel and chitosan nanofibers have been proceeded recently, especially for adapting to water purification and air filtration.

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Section: Chitosan Nanofibermentioning

confidence: 99%

Innovative Separation Technology Utilizing Marine Bioresources: Multifaceted Development of a Chitosan-Based System Leading to Environmentally-Friendly Processes

Kashima¹,

Takahashi²,

Nakayama³

et al. 2021

Chitin and Chitosan - Physicochemical Properties and Industrial Applications [Working Title]

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“…The prepared Fe 3+ ions’ immobilized nanofiber fabric presented a high lysozyme adsorption capacity of 365 mg/g. Reactive dyes can interact with the active sites of many proteins by mimicking the structure of the substrate or cofactors of these proteins and are excellent ligand candidates for affinity [ 36 ]. Ng et al [ 37 ] developed a chitosan and Reactive Orange 4 modified polyacrylonitrile nanofiber fabric.…”

Section: Introductionmentioning

confidence: 99%

Selective Adsorption and Separation of Proteins by Ligand-Modified Nanofiber Fabric

Liu

Mukai

2021

Polymers

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Electrospun polyvinyl alcohol (PVA) nanofiber fabric was modified by Cibacron Blue F3GA (CB) to enhance the affinity of the fabric. Batch experiments were performed to study the nanofiber fabric’s bovine hemoglobin (BHb) adsorption capacity at different protein concentrations before and after modification. The maximum BHb adsorption capacity of the modified nanofiber fabric was 686 mg/g, which was much larger than the 58 mg/g of the original fabric. After that, the effect of feed concentration and permeation rate on the dynamic adsorption behaviors for BHb of the nanofiber fabric was investigated. The pH impact on BHb and bovine serum albumin (BSA) adsorption was examined by static adsorption experiments of single protein solutions. The selective separation experiments of the BHb–BSA binary solution were carried out at the optimal pH value, and a high selectivity factor of 5.45 for BHb was achieved. Finally, the reusability of the nanofiber fabric was examined using three adsorption–elution cycle tests. This research demonstrated the potential of the CB-modified PVA nanofiber fabric in protein adsorption and selective separation.

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“…Desirable features of nanofiber membranes intended for use in protein adsorption include low non-specific adsorption, high adsorption capacity, high adsorption rate, good biocompatibility, and high chemical/mechanical stability; these characteristics can be achieved by manipulation of chemical composition and synthesis operation that result in high porosity, large specific adsorption area, and good hydrophilicity of nanofiber membranes [6,[9][10][11][12][13]. Polyacrylonitrile (PAN) membranes have superior mechanical strength and chemical stability [14,15], but their hydrophobic nature makes them less suitable for application in protein adsorption, as compared with the cellulose membranes that have good hydrophilicity [16,17].…”

Section: Introductionmentioning

confidence: 99%

“…Immobilization of dye ligand onto the PAN-based nanofiber membranes has been proven effective in selective adsorption of target proteins [11][12][13]. Among different types of dyes, Cibacron blue F3GA (abbreviated as CB, or known as reactive blue 2 dye) reactive dye is the one widely utilized in protein adsorption [15,18].…”

Section: Introductionmentioning

confidence: 99%

Kinetic and Thermodynamic Studies of Lysozyme Adsorption on Cibacron Blue F3GA Dye-Ligand Immobilized on Aminated Nanofiber Membrane

et al. 2021

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The polyacrylonitrile (PAN) nanofiber membrane was prepared by the electrospinning technique. The nitrile group on the PAN nanofiber surface was oxidized to carboxyl group by alkaline hydrolysis. The carboxylic group on the membrane surface was then converted to dye affinity membrane through reaction with ethylenediamine (EDA) and Cibacron Blue F3GA, sequentially. The adsorption characteristics of lysozyme onto the dye ligand affinity nanofiber membrane (namely P-EDA-Dye) were investigated under various conditions (e.g., adsorption pH, EDA coupling concentration, lysozyme concentration, ionic strength, and temperature). Optimum experimental parameters were determined to be pH 7.5, a coupling concentration of EDA 40 μmol/mL, and an immobilization density of dye 267.19 mg/g membrane. To understand the mechanism of adsorption and possible rate controlling steps, a pseudo first-order, a pseudo second-order, and the Elovich models were first used to describe the experimental kinetic data. Equilibrium isotherms for the adsorption of lysozyme onto P-EDA-Dye nanofiber membrane were determined experimentally in this work. Our kinetic analysis on the adsorption of lysozyme onto P-EDA-Dye nanofiber membranes revealed that the pseudo second-order rate equation was favorable. The experimental data were satisfactorily fitted by the Langmuir isotherm model, and the thermodynamic parameters including the free energy change, enthalpy change, and entropy change of adsorption were also determined accordingly. Our results indicated that the free energy change had a negative value, suggesting that the adsorption process occurred spontaneously. Moreover, after five cycles of reuse, P-EDA-Dye nanofiber membranes still showed promising efficiency of lysozyme adsorption.

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Batch and dynamic adsorption of lysozyme from chicken egg white on dye-affinity nanofiber membranes modified by ethylene diamine and chitosan

Cited by 32 publications

References 48 publications

Innovative Separation Technology Utilizing Marine Bioresources: Multifaceted Development of a Chitosan-Based System Leading to Environmentally-Friendly Processes

Innovative Separation Technology Utilizing Marine Bioresources: Multifaceted Development of a Chitosan-Based System Leading to Environmentally-Friendly Processes

Selective Adsorption and Separation of Proteins by Ligand-Modified Nanofiber Fabric

Kinetic and Thermodynamic Studies of Lysozyme Adsorption on Cibacron Blue F3GA Dye-Ligand Immobilized on Aminated Nanofiber Membrane

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