Basophil-bound and serum immunoglobulin E directed against Helicobacter pylori in patients with chronic gastritis

Aceti, Antonio; Celestino, D; Caferro, Marta; Casale, V.; Citarda, F; Conti, Ettore; Grassi, Antonio; Grilli, Anastasio; Pennica, Alfredo; Sciarretta, F.; Leri, O; Ameglio, Franco; Sebastiani, A.

doi:10.1016/0016-5085(91)90469-2

Cited by 62 publications

(30 citation statements)

References 30 publications

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“…Of a total of 25 chronic urticaria patients with current H. pylori infection, only 1 patient appeared positive with H. pylori -specific RAST. This indicates that IgE-mediated responses against H. pylori are not common in this group of patients and may not be involved in histamine release, for example, contrary to earlier observations by Aceti et al [6], who found basophil-bound specific IgE in 84% and serum IgE, determined by the histamine release test, in 69% of patients with H. pylori infection.…”

Section: Discussioncontrasting

confidence: 54%

“…In addition to IgG and IgA, the IgE-mediated immune response to H. pylori has been studied [6, 7, 8], but little is known about the antigenic structures of H. pylori that are responsible for the binding of IgE antibodies. In previous reports, we found elevated total IgE levels in patients with chronic urticaria, especially in patients with H. pylori gastritis [9].…”

Section: Introductionmentioning

confidence: 99%

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Extraction of IgE-Binding Components of <i>Helicobacter pylori</i> by Immunoblotting Analysis in Chronic Urticaria Patients

Liutu

Kalimo

Uksila

et al. 2001

Int Arch Allergy Immunol

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Background: An association between Helicobacter pylori and chronic urticaria has been suspected previously. An IgE-mediated pathway might be a possible link between H. pylori infection and chronic urticaria, and therefore we wanted to prepare an optimal H. pylori antigen to detect H. pylori-specific IgE antibodies in chronic urticaria patients. Methods:H. pylori antigen extracts were prepared in different ways to find the optimal antigen extract to be used in the assays. Immunoblotting was used to detect IgE-binding bands. The results were applied in an H. pylori RAST assay for specific H. pylori IgE antibodies in patient sera. Results: In immunoblotting, the largest number of IgE-stained bands were visualized in the washing fluids and sonicated extracts, while strong heating and denaturing treatments destroyed the epitopes for IgE binding, suggesting that they belonged to the flagellar structures of H. pylori. However, in H. pylori-specific RAST analysis, specific IgE was found only in 1 of 25 H. pylori-infected patients. Conclusions: Our findings suggest that although IgE-binding epitopes were found in H. pylori, H. pylori-specific IgE antibodies are not common in chronic urticaria, and the clinical significance of the IgE response is unclear.

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Section: Discussioncontrasting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Extraction of IgE-Binding Components of <i>Helicobacter pylori</i> by Immunoblotting Analysis in Chronic Urticaria Patients

Liutu

Kalimo

Uksila

et al. 2001

Int Arch Allergy Immunol

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“…For example, a mechanism by which basophils may be activated in H. pylori gastritis could implicate IL-8, which is secreted from inflamed mucosa (80) and is able to induce histamine release from basophils (81). In addition, it cannot be excluded that basophils from H. pylori-positive individuals can be activated through an IgE-mediated mechanism (37,39).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, H. pylori is involved in mast cell chemotaxis, and activation and release of proinflammatory mediators and cytokines. Although peripheral blood basophils may be increased in H. pylori-positive patients (36), studies of the in vitro effects of H. pylori on histamine release from basophils have yielded inconclusive results (37)(38)(39).…”

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confidence: 99%

Basophils Infiltrate Human Gastric Mucosa at Sites of Helicobacter pylori Infection, and Exhibit Chemotaxis in Response to H. pylori-derived Peptide Hp(2–20)

Paulis

Prevete

Fiorentino

et al. 2004

The Journal of Immunology

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Basophils, which are normally confined to the circulation, can migrate to sites of allergic inflammation. Using the specific mAb, BB1, we detected basophil infiltration of the gastric mucosa of Helicobacter pylori-infected patients affected by moderate and severe gastritis. Basophils were not found in H. pylori-free individuals or in subjects with mild gastritis. The H. pylori-derived peptide, Hp(2–20), was a potent basophil chemoattractant in vitro, whereas the control peptide, Hp1, was ineffective. Basophils from peripheral blood of healthy volunteers expressed mRNA for the formyl peptide receptors, N-formyl-peptide receptor (FPR), FPR-like (FPRL)1, and FPRL2. Preincubation of basophils with FMLP or Hp(2–20) caused complete desensitization to a subsequent challenge with homologous stimulus. Incubation of basophils with a low concentration of FMLP, which binds with high affinity to FPR, but not to FPRL1 or FPRL2, did not affect the chemotactic response to Hp(2–20). In contrast, a high concentration of FMLP, which binds to FPRL1 and FPRL2, reduced the chemotactic response to Hp(2–20). The FPR antagonist, cyclosporin H, prevented chemotaxis induced by FMLP, but not by Hp(2–20). Hp(2–20) could be responsible, at least in part, for basophil infiltration of the gastric mucosa of H. pylori-infected patients presumably through the interaction with FPRL1 and FPRL2.

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“…Histamine liberation, by an IgE-mediated mechanism, was similarly achieved when normal basophils were passively sensitised with serum from IgE-positive patients. 16 In-vitro studies have shown that washed cells, formalin-killed cells and crude cell-wall preparations of H. pylori were unable to induce histamine release from rat serosal, peritoneal and pleural mast cells. However, similar preparations from several H .…”

Section: Introductionmentioning

confidence: 99%

Modulatory action of Helicobacter pylori on histamine release from mast cells and basophils in vitro

Lutton

Bamford

O'Loughlin

et al. 1995

Journal of Medical Microbiology

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Helicobacter pylori is important in the aetiology of peptic ulceration. Despite inducing an inflammatory response in the mucosa, the organism persists, suggesting that it has efficient protective mechanisms. Some bacterial and viral products modulate histamine secretion from inflammatory cells. Therefore, this study examined the modulatory effects of H. pylori preparations on histamine release from rat peritoneal mast cells and human basophils. Eleven clinical isolates of H. pylori were prepared in different ways: as whole washed bacteria, washed sonicated bacteria, and formalin-killed bacteria, and as outermembrane and lipopolysaccharide (LPS) extracts. Histamine release from mast cells or basophils was not elicited by any of these bacterial preparations alone. However, when mixed with various secretory stimulants, the bacterial preparations caused inhibition of histamine release from rat mast cells (calcium ionophore A23187, compound 48/80, concanavalin A, anti-rat IgE) and human basophils (A23187, N-formyl Met-Leu-Phe). The degree of inhibition ranged from 48% to 97%. These results indicate that H. pylori exerts an inhibitory effect on cells of the immune system that contributes to its persistence within the gastric mucosa.

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Basophil-bound and serum immunoglobulin E directed against Helicobacter pylori in patients with chronic gastritis

Cited by 62 publications

References 30 publications

Extraction of IgE-Binding Components of <i>Helicobacter pylori</i> by Immunoblotting Analysis in Chronic Urticaria Patients

Extraction of IgE-Binding Components of <i>Helicobacter pylori</i> by Immunoblotting Analysis in Chronic Urticaria Patients

Basophils Infiltrate Human Gastric Mucosa at Sites of Helicobacter pylori Infection, and Exhibit Chemotaxis in Response to H. pylori-derived Peptide Hp(2–20)

Modulatory action of Helicobacter pylori on histamine release from mast cells and basophils in vitro

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