Basophil allergen threshold sensitivity, CD‐sens, IgE‐sensitization and DBPCFC in peanut‐sensitized children

Glaumann, Susanne; Nopp, Anna; Johansson, S. G. O.; Rudengren, Magnus; Borres, Magnus P.; Nilsson, Caroline

doi:10.1111/j.1398-9995.2011.02754.x

Cited by 121 publications

(144 citation statements)

References 25 publications

(64 reference statements)

Supporting

Mentioning

139

Contrasting

Unclassified

Order By: Relevance

“…Basophil allergen threshold sensitivity, CD-sens [20], combines CD203c (for basophil identification) and CD63 (for basophil activation) and has been shown to mirror the target organ allergen sensitivity, and an excellent correlation was found with bronchial allergen threshold sensitivity [21]. Previous studies comparing CD-sens and skin prick test titration [20], nasal allergen challenge [20] and food challenges also indicated a good agreement [22]. Basophil activation tests measuring cell reactivity have also been evaluated in milk, egg and wheat allergy [23,24,25,] and, recently, Chinuki et al [16] showed that basophil reactivity could be useful in differentiating between subtypes of WDEIA caused by wheat and/or by HWP.…”

Section: Introductionmentioning

confidence: 82%

“…This varied response has been described previously [22,23,24]. The consequence of this is that an oral challenge has to be performed on individuals who are low responders or nonresponders in the CD-sens test [22]. …”

Section: Discussionmentioning

confidence: 99%

“…For individuals with a response between 5 and 16% (low responders), the results should be interpreted with caution. The cutoff 16% was calculated (mean 76% -3 SD) from the positive controls of an in-house reference material of 264 allergic children and adults [22]. …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Combining Analyses of Basophil Allergen Threshold Sensitivity, CD-sens, and IgE Antibodies to Hydrolyzed Wheat, ω-5 Gliadin and Timothy Grass Enhances the Prediction of Wheat Challenge Outcome

Nilsson

Hedlin

et al. 2013

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Background: Wheat is a common food causing allergy which has implications on the quality of life. The diagnosis of IgE-mediated wheat allergy is based on the clinical history and presence of IgE antibodies (IgE-Ab) in skin or blood, and the results of an oral food challenge which is time consuming and associated with risks. An improved diagnostic workup is needed for wheat allergy. The objective was to examine the relationship between wheat challenge, CD-sens and IgE-Ab to related allergens in wheat-allergic children and investigate if a combination of different markers could enhance the prediction of challenge outcome. Method: Twenty-four children (aged 1-15 years) with a wheat allergy diagnosis underwent an open wheat challenge. CD-sens and IgE-Ab to wheat, hydrolyzed wheat protein (HWP), ω-5 gliadin and timothy grass were analyzed and related to the challenge outcome. Results: A positive challenge was seen in 12/24 children. Children reacting to the challenge had higher IgE-Ab concentrations to wheat, ω-5 gliadin and HWP (p < 0.01) and a tendency to higher wheat CD-sens values (p = 0.08) than nonreacting children. Combining wheat CD-sens >150 and IgE-Ab to wheat >20 kU_A/l, or ω-5 gliadin >0.1 kU_A/l predicted the challenge outcome in 83% of the patients. Most children with IgE-Ab to wheat also had IgE-Ab to timothy. Seven of 9 challenge-positive children had a positive CD-sens to HWP and IgE-Ab to HWP >8 kU_A/l. Conclusion: Combining CD-sens and IgE-Ab to wheat or wheat components could be useful in the diagnosis and follow-up of wheat-allergic children.

show abstract

Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Combining Analyses of Basophil Allergen Threshold Sensitivity, CD-sens, and IgE Antibodies to Hydrolyzed Wheat, ω-5 Gliadin and Timothy Grass Enhances the Prediction of Wheat Challenge Outcome

Nilsson

Hedlin

et al. 2013

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…By an in‐vitro dose‐response allergen stimulation of the basophils, their allergen threshold sensitivity, CD‐sens 16 can be accurately determined. Studies have shown that CD‐sens correlates significantly with bronchial allergen threshold sensitivity 9 , nasal allergen challenge 14, 18 , and oral food challenge 15, 19, 20 .…”

Section: Discussionmentioning

confidence: 99%

A clinical follow‐up of omalizumab in routine treatment of allergic asthma monitored by CD‐sens

Johansson

Lilja

Hallberg

et al. 2018

Immunity Inflam & Disease

View full text Add to dashboard Cite

IntroductionOmalizumab has been available for treatment of allergic asthma for more than a decade and thus, its efficacy in routine treatment was of interest to evaluate. Basophil allergen threshold sensitivity (CD‐sens) has been shown to correlate with the bronchial allergen threshold sensitivity and can be used to objectively measure omalizumab treatment efficacy. We aimed to evaluate the effect of omalizumab treatment of allergic asthma by CD‐sens, as an objective marker of the IgE‐mediated inflammation, and related to SPT, spirometry, FeNO, Asthma Control Questionnaire (ACQ), and Global Evaluation of Treatment Effectiveness (GETE).MethodsThirty‐two patients were treated with omalizumab for 16 weeks. CD‐sens was used to define the response and related to clinical parameters. If CD‐sens was negative (<0.1) (CD‐sens low Group) the patient continued with the standard dose. If CD‐sens was ≥0.1 (CD‐sens high Group) a second 16 weeks period with 25–50% dosage increase was started and evaluated after a total of 32 weeks.ResultsNine of 32 patients became CD‐sens negative after treatment (CD‐sens start: 8.0; 16 weeks: <0.01) and regarded as successful. 15/23 were unsuccessful (CD‐sens start: 13; 16 weeks: 1.65) and the omalizumab dose was increased. CD‐sens decreased significantly (p < 0.05) and further 3/15 patients became CD‐sens negative (CD‐sens at 32 weeks: 0.5). There was a significantly smaller IgE‐ab fraction (IgE‐ab/IgE) in the CD‐sens low versus the CD‐sens high Group (p < 0.0001). A significant decrease in ACQ was seen in both groups after 16 weeks treatment (p = 0.05 and 0.01, respectively). No significant changes could be detected for the other clinical parameters.ConclusionBy the use of the objective laboratory method CD‐sens, which effectively measure the direct effect of omalizumab, that is, the IgE‐mediated part of the allergic asthma, in combination with clinical parameters it might be possible to more effectively monitor and treat IgE‐mediated allergic asthma.

show abstract

“…Thus, the basophil activation test (BAT) may offer a clinical answer to an allergen [15] and a complement to the severity of the clinical picture. There are few studies that perform BATs in peanut food allergy [16,17]. …”

Section: Introductionmentioning

confidence: 99%

The Relevance of Basophil Allergen Sensitivity Testing to Distinguish between Severe and Mild Peanut-Allergic Children

Homšak

Šilar

Berce

et al. 2013

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Peanut sensitization is common in children. However, it is difficult to assess which children will react mildly and which severely. This study evaluated the relevance of basophil allergen sensitivity testing to distinguish the severity of peanut allergy in children. Methods: Twenty-seven peanut-sensitized children with symptoms varying from mild symptoms to severe anaphylaxis underwent peanut CD63 dose-response curve analysis with the inclusion of basophil allergen sensitivity calculation (CD-sens) and peanut component immunoglobulin E (IgE) testing. Results: Eleven children who had experienced anaphylaxis to peanuts showed a markedly higher peanut CD63 response at submaximal allergen concentrations and CD-sens (median 1,667 vs. 0.5; p < 0.0001) than 16 children who experienced a milder reaction. Furthermore, a negative or low CD-sens to peanuts unambiguously excluded anaphylactic peanut allergy. Children with anaphylaxis have higher levels of Ara h 1, 2, 3 and 9 IgE, but comparable levels of IgE to Ara h 8 and whole-peanut extract. The diagnostic specificity calculated with a receiver operating characteristic analysis reached 100% for CD-sens and 73% for Ara h 2. Conclusions: We demonstrated that severe peanut allergy is significantly associated with higher basophil allergen sensitivity. This cellular test should facilitate a more accurate diagnosis of peanut allergy.

show abstract

Basophil allergen threshold sensitivity, CD‐sens, IgE‐sensitization and DBPCFC in peanut‐sensitized children

Abstract: In this study, a negative CD-sens to peanut excluded peanut allergy. Both tests, CD-sens to peanut and immunoassay for IgE-antibody to the peanut components, appear to be safe, time saving and cost-effective complements to DBPCFC.

Cited by 121 publications

References 25 publications

Combining Analyses of Basophil Allergen Threshold Sensitivity, CD-sens, and IgE Antibodies to Hydrolyzed Wheat, ω-5 Gliadin and Timothy Grass Enhances the Prediction of Wheat Challenge Outcome

Combining Analyses of Basophil Allergen Threshold Sensitivity, CD-sens, and IgE Antibodies to Hydrolyzed Wheat, ω-5 Gliadin and Timothy Grass Enhances the Prediction of Wheat Challenge Outcome

A clinical follow‐up of omalizumab in routine treatment of allergic asthma monitored by CD‐sens

The Relevance of Basophil Allergen Sensitivity Testing to Distinguish between Severe and Mild Peanut-Allergic Children

Contact Info

Product

Resources

About

Basophil allergen threshold sensitivity, CD‐sens, IgE‐sensitization and DBPCFC in peanut‐sensitized children

Abstract: In this study, a negative CD-sens to peanut excluded peanut allergy. Both tests, CD-sens to peanut and immunoassay for IgE-antibody to the peanut components, appear to be safe, time saving and cost-effective complements to DBPCFC.

Cited by 121 publications

References 25 publications

Combining Analyses of Basophil Allergen Threshold Sensitivity, CD-sens, and IgE Antibodies to Hydrolyzed Wheat, &#x03C9;-5 Gliadin and Timothy Grass Enhances the Prediction of Wheat Challenge Outcome

Combining Analyses of Basophil Allergen Threshold Sensitivity, CD-sens, and IgE Antibodies to Hydrolyzed Wheat, &#x03C9;-5 Gliadin and Timothy Grass Enhances the Prediction of Wheat Challenge Outcome

A clinical follow‐up of omalizumab in routine treatment of allergic asthma monitored by CD‐sens

The Relevance of Basophil Allergen Sensitivity Testing to Distinguish between Severe and Mild Peanut-Allergic Children

Contact Info

Product

Resources

About

Combining Analyses of Basophil Allergen Threshold Sensitivity, CD-sens, and IgE Antibodies to Hydrolyzed Wheat, ω-5 Gliadin and Timothy Grass Enhances the Prediction of Wheat Challenge Outcome

Combining Analyses of Basophil Allergen Threshold Sensitivity, CD-sens, and IgE Antibodies to Hydrolyzed Wheat, ω-5 Gliadin and Timothy Grass Enhances the Prediction of Wheat Challenge Outcome