Basil oil-nanoemulsions: Development, cytotoxicity and evaluation of antioxidant and antimicrobial potential

Gündel, Samanta da Silva; Velho, Maiara Callegaro; Diefenthaler, Marianne Kerche; Favarin, Fernanda Reis; Copetti, Priscila Marquezan; Fogaça, Aline de Oliveira; Klein, Bruna; Wagner, Róger; Gündel, André; Sagrillo, Michele Rorato; Ourique, Aline Ferreira

doi:10.1016/j.jddst.2018.05.038

Cited by 55 publications

(34 citation statements)

References 20 publications

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“…After preparation, the formulations were characterized according to medium particle size, polydispersity index, zeta potential, pH, active content, encapsulation efficiency, and morphology (Coradini et al, 2014;Gündel, Velho, et al, 2018;Rossi et al, 2017;Zanotto-Filho et al, 2013).…”

Section: Nanocapsule Characterizationmentioning

confidence: 99%

“…Morphological analysis of the nanocapsules was performed using the technique of atomic force microscopy following the methodology described by Gündel, Velho, et al (2018), with some modifications. After dilution of the samples into ultrapure water (1:50), a drop was added to a cleaved mica surface, and then the drying was performed.…”

Section: Nanocapsule Characterizationmentioning

confidence: 99%

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Low‐dose curcumin‐loaded Eudragit L‐100‐nanocapsules in the diet of dairy sheep increases antioxidant levels and reduces lipid peroxidation in milk

et al. 2019

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The main objective of this study was to evaluate whether the addition of curcumin‐loaded nanocapsules (prepared and characterized) in the diets of dairy sheep improved milk quality. The nanocapsules were prepared using two polymers: poly‐ε‐caprolactone (PCL) and Eudragit L‐100. The nanocapsules contained 0.25 mg/ml (Nano‐Eudragit L‐100) and 2 mg/ml (Nano‐PCL) of curcumin. Dairy sheep were divided into four groups: A (control), B (30 mg free curcumin/kg concentrate), C (3 mg Nano‐PCL/kg concentrate), and D (3 mg Nano‐Eudragit/kg concentrate). We observed that the number of total leukocytes and serum globulin levels were lower in Group D than in the control (Group A) (p < 0.05). Antioxidant capacity against peroxyl radicals (ACAP) and catalase enzymes was elevated in Group D, with consequently reduced lipid peroxidation (LPO; p < 0.05). In milk, there were no differences in production and composition between groups during the experimental period (p > 0.05); however, ACAP increased and LPO decreased in milk. Practical applications Curcumin is a functional molecule with potent antioxidant, anti‐inflammatory, and antimicrobial actions, used frequently and with medical indications in human food. Free curcumin in sheep diets improves milk quality and increases its shelf life. This study showed that curcumin nanocapsules produced from the Eudragit L‐100 polymer potentiated the anti‐inflammatory and antioxidant actions of dairy sheep when used in the diet daily, at doses 10 times lower than that of free curcumin. These positive effects were reflected in higher total antioxidant capacity and lower lipid peroxidation in milk in sheep‐fed curcumin‐loaded Eudragit L‐100 nanocapsules, generating desirable milk properties. In practice, the use of nanotechnology enhances the beneficial effects of curcumin in milk, possibly creating a nutraceutical food desirable to consumers.

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Section: Nanocapsule Characterizationmentioning

confidence: 99%

Section: Nanocapsule Characterizationmentioning

confidence: 99%

Low‐dose curcumin‐loaded Eudragit L‐100‐nanocapsules in the diet of dairy sheep increases antioxidant levels and reduces lipid peroxidation in milk

et al. 2019

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“…The use of natural compounds in the formulation of food and pharmacologic products is very interesting and the essential oils are the most important natural additives due to their organoleptic, biological, and therapeutic properties (Calo, Crandall, O'Bryan, & Ricke, ; da Silva Gündel et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…In the recent years, many researchers have been investigating the potential of nanoemulsified essential oils (Ahmad et al, ; Bhargava, Conti, da Rocha, & Zhang, ; da Silva Gündel et al, ; Ghosh, Mukherjee, & Chandrasekaran, , ). The reduction of the droplet size of emulsions can be done by high‐energy methods, such as ultrasound cavitation.…”

Section: Introductionmentioning

confidence: 99%

“…The nanodroplet formed is surrounded by the stabilizer and acts as a vehicle for hydrophobic components delivery, such as the essential oils, and further improving the solubilization of the oil in aqueous continuous phases such as cosmetics or food formulations (Ahmad et al, ; Bhargava et al, ; da Silva Gündel et al, ; Ghosh et al, , ). In addition, the Brownian motion effects present in nanoemulsions (NEs) dominate the gravitational forces due to the very low droplet average diameter and the range of steric repulsion is less dependent of the particle size, ensuring great stability against aggregation effects (Ranjan et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Production of clove oil nanoemulsion with rapid and enhanced antimicrobial activity against gram‐positive and gram‐negative bacteria

Meneses

Sayer

Puton

et al. 2019

J Food Process Engineering

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The clove essential oil is a natural antioxidant and antimicrobial with potential applications in many fields of the industry. Nanoemulsions (NEs) with Dp ~135 nm loaded with clove oil (CO) and crodamol were produced by ultrasound. The presence of crodamol in formulation increased droplet size homogeneity and prevented the diffusional degradation with the consequent increase in the stability. The formulation containing 7 mg/mL of CO presented a high surface area and exhibited the lowest minimum inhibitory concentration against gram‐positive and negative bacteria when compared to the free CO, indicating an increase in the antimicrobial activity with the reduction of the droplet size to the nanometric range. Microbial inhibition kinetics showed a fast action of the NE in the inactivation of microorganisms tested. The antioxidant activity was evaluated and both free and nanoemulsified CO remained with antioxidant potential around 90%. The present work produced CO NE with rapid and increased bioactivity, and strong antioxidant potential, becoming an effective and natural alternative for future applications in food formulations. Practical applications The clove essential oil is very interesting to the food and pharmaceutical industries due to the great antimicrobial and antioxidant properties. However, some limitations are related to its hydrophobic character, which limits the application in food formulations due to the low solubility in aqueous media. The essential oils nanoemulsions can be a great alternative to improve the oil solubility, increase the contact area, and protect the bioactive components that are present in the essential oil. Although some studies have been showing the great potential of the essential oil nanoemulsions, the investigation around the reduction of the emulsion droplet diameter, considering the relation between the nanometric range and the increase in the bioavailability and biological activity, is a critical issue to enable their application in food formulations.

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Plant‐based essential oil encapsulated in nanoemulsions and their enhanced therapeutic applications: An overview

Jamir,

Bhushan,

Sanjukta

et al. 2023

Biotech & Bioengineering

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In recent years, studies on the formulation of nanoemulsions have been the focus of attention due to their potential applicability in food, pharmaceuticals, cosmetics, and agricultural industries. Nanoemulsions can be formulated using ingredients approved by the Food and Drug Administration (FDA), which assures their safety profiles to a great extent. Bioactive compounds such as essential oils although have strong biological properties and antimicrobial compounds, their usage is restricted due to their high volatility, instability, and hydrophobic nature. Therefore, nanoemulsion as carrier vehicle can be used to encapsulate essential oils to obtain stable and enhanced physicochemical characteristics of the essential oils. This review details the structure, formulation, and characterization techniques used for nanoemulsions, with a focus on the essential oil‐based nanoemulsions which have the potential to be used as antimicrobial and anticancer therapeutics.

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Basil oil-nanoemulsions: Development, cytotoxicity and evaluation of antioxidant and antimicrobial potential

Cited by 55 publications

References 20 publications

Low‐dose curcumin‐loaded Eudragit L‐100‐nanocapsules in the diet of dairy sheep increases antioxidant levels and reduces lipid peroxidation in milk

Low‐dose curcumin‐loaded Eudragit L‐100‐nanocapsules in the diet of dairy sheep increases antioxidant levels and reduces lipid peroxidation in milk

Production of clove oil nanoemulsion with rapid and enhanced antimicrobial activity against gram‐positive and gram‐negative bacteria

Plant‐based essential oil encapsulated in nanoemulsions and their enhanced therapeutic applications: An overview

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