Basic Principles of Mastitis Therapy

Radinović, Miodrag; Davidov, Ivana; Kovačević, Zorana; Stojanović, Dragica; Galfi, Annamaria; Erdeljan, Mihajlo

doi:10.7251/vetjen1901110r

Cited by 3 publications

(4 citation statements)

References 5 publications

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Section: Resultsmentioning

confidence: 99%

“…Antibiotics included in the testing are amoxycillin (AMX), ampicillin (AMP), ceftriaxone (CRO), enrofloxacin (ENR), erythromycin (ERY), lincomycin (LIN), neomycin (NEO), penicillin (PEN), streptomycin (STR), tetracycline (TET), amoxicillin/clavulanic acid (AMC), novobiocin (NB), trimethoprim/sulfamethoxazole (SXT), and cloxacillin (CLO). In Serbia, the most used antibiotics in mastitis therapy are penicillin, streptomycin, gentamicin, tetracycline, cephalexin, sulfonamides, and enrofloxacin [31]. Application of correspondence analysis (CA) on a dataset describing bactericidal potential of different antibiotics on bacteria isolated from the milk samples shows associations of different bacteria and the evaluated antibiotics in terms of bacteria resistance (R) or sensitivity (S).…”

Section: Antibiotic Susceptibility Testing Of Mastitis-associated Bacteriamentioning

confidence: 99%

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Natural Agents against Bovine Mastitis Pathogens

et al. 2021

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Bovine mastitis is the most widespread and economically important disease worldwide. The present study aimed to determine bioactive compounds in two essential oils (EOs) from wild (Thymus serpyllum) and common thyme (Thymus vulgaris) and to assess the antioxidant potential as well as antibacterial efficacy of the EOs against mastitis-associated bacteria. The study also included antibiotic susceptibility tests. The strains were previously isolated from lactating animals with clinical and subclinical mastitis. The antioxidant potential of the commercial EOs of wild and common thyme was evaluated by five in vitro assays. The antibacterial activity was performed using the microdilution technique, while antibiotic susceptibility testing was performed by the Kirby–Bauer disc diffusion method. The dominant compound in wild thyme was thymol (45.22%), followed by p-cymene (23.83%) and γ-terpinene (3.12%), while in common thyme, it was thymol (54.17%), followed by γ-terpinene (22.18%) and p-cymene (16.66%). Among the fourteen mastitis-associated bacteria, strain IX Streptococcus spp. (β-hemolytic) was the most sensitive to the tested EOs (minimum inhibitory concentration (MIC)/minimal bactericidal concentration (MBC) were 0.78/1.56 and 0.39/0.78 mg/mL for T. serpyllum (TS) and T. vulgaris (TV), respectively). Regarding Streptococcus spp. β heamoliticus, MICs for TS ranged from 0.78 to 1.56 mg/mL, while for the same oil, MBCs ranged from 1.56 to 12.5 mg/mL. In the case of T. vulgaris, MICs ranged from 0.39 to 3.125 mg/ mL, while MBCs ranged from 3.125 to 6.25 mg/mL. TV is more active against E. coli, E. sakazakii, and Streptococcus spp., while it is less effective against Staphylococcus spp. than TS. The study revealed that the tested EOs possess remarkable antioxidative and antibacterial activities and could be used in the development of pharmaceutical formulation as an alternative to conventional mastitis therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Antibiotic Susceptibility Testing Of Mastitis-associated Bacteriamentioning

confidence: 99%

Natural Agents against Bovine Mastitis Pathogens

et al. 2021

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“…Antibiotics included in the antibiotic susceptibility testing were amoxycillin, ampicillin, ceftriaxone, enrofloxacin, erythromycin, lincomycin, neomycin, penicillin, streptomycin, tetracycline, amoxicillin/clavulanic acid, novobiocin, trimethoprim/sulfamethoxazole, and cloxacillin. The current data suggests that the most commonly used antibiotics in mastitis therapy in Serbia are penicillin, streptomycin, gentamicin, tetracycline, cephalexin, sulfonamides, and enrofloxacin [ 21 , 22 , 23 ]. According to the data obtained in this study, all mastitis associated pathogens are resistant to penicillin, which is not surprising, considering the previously stated fact.…”

Section: Resultsmentioning

confidence: 99%

New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives

et al. 2021

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Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.

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“…Antibiotics are frequently used to treat mastitis, intramammary or parenterally (Erskine et al., 2003; Suojala et al., 2013). Intramammary formulations often include either one or multiple agents (Pașca et al., 2020), while injections usually contain single antibiotic (Radinović et al., 2019). In addition to antibiotics, supportive and symptomatic therapy (non‐steroidal anti‐inflammatory drugs, corticosteroids, vitamins, cytokines, and vaccines) are applied with the aim of relieving symptoms, improving recovery and milk production (Cheng & Han, 2020; Smulski et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Assessment of antibiotic use patterns in bovine mastitis treatment in the dairy sector in Serbia

Tomanić,

Samardžija,

Kladar

et al. 2023

Reprod Domestic Animals

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The dairy sector is essential for the world's food systems, playing a vital role in agricultural production. Yet, mastitis, an inflammation of the mammary gland, affects a considerable portion of the dairy cow population annually, leading to reduced productivity and sustainability. Insight into therapeutic protocols is essential for a better understanding of the situation on farms with the aim of developing new or harmonizing existing protocols in the mastitis treatment. This study conducted on dairy farm in Serbia aimed to explore the most commonly used antibiotics during bovine mastitis treatment and their association with daily milk yield. Data from 100 lactating cows, including therapeutic protocols, lactation numbers, mastitis episodes, milk yield, and antibiotic usage, were obtained from a computerized database and statistically analysed. The results demonstrated a high prevalence of narrow‐spectrum antibiotics usage, with the majority of cows treated with combination therapy. The route of antibiotic application did not significantly impact daily milk yield, while treatment duration had no discernible effect. Nonetheless, specific antibiotics were associated with milk yield variations, suggesting the need for careful antibiotic selection and management in mastitis therapy. These findings emphasize the importance of prudent antibiotic use to safeguard animal health and long‐term milk production sustainability.

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Basic Principles of Mastitis Therapy

Cited by 3 publications

References 5 publications

Natural Agents against Bovine Mastitis Pathogens

Natural Agents against Bovine Mastitis Pathogens

New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives

Assessment of antibiotic use patterns in bovine mastitis treatment in the dairy sector in Serbia

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