Basic fibroblast growth factor is critical to reprogramming buffalo ( Bubalus bubalis ) primordial germ cells into embryonic germ stem cell-like cells

Wang, Caizhu; Deng, Yanfei; Chen, Feng; Zhu, Peng; Wei, Jingwei; Luo, Chen; Lu, Fenghua; Yang, Sufang; Shi, Dongquan

doi:10.1016/j.theriogenology.2016.12.035

Cited by 2 publications

(3 citation statements)

References 47 publications

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“…Our results show that mRNA expression and the secretion of bFGF and VEGF were improved in the hypoxic‐cultured bASCs. Our previous studies have found bFGF plays an important role in buffalo iPSCs and germ stem cell‐like cells (Deng et al, ; Wang et al, ), suggesting that hypoxia might improve proliferation and stemness of bASCs through enhanced secretion of bFGF and VEGF.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the antiapoptotic gene Bcl-2, and proliferation gene Ki-67 were significantly upregulated in bASCs cultured under hypoxic conditions. Expression of pluripotency-related genes are essential for the maintenance of pluripotency, self-renewal, and proliferation in iPSCs and germ stem cell-like cells (Deng et al, 2012;Wang et al, 2017), suggesting that hypoxia might improve proliferation and stemness of bASCs through enhanced secretion of bFGF and VEGF.…”

Section: Hypoxia Promotes Reprogramming Of Bascs Into Ipscsmentioning

confidence: 99%

“…These characteristics can be attributed to the special features of bASCs and the special hypoxic cell culture conditions utilized in this study. bASCs secrete many growth factors, such as bFGF, which is important for maintenance of self-renewal capacity and pluripotency in buffalo pluripotent stem cells (Deng et al, 2012;Wang et al, 2017). bASCs also express the key pluripotency reprogramming genes OCT4, SOX2, KLF4, C-MYC, and NANOG.…”

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confidence: 99%

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Hypoxia enhances buffalo adipose‐derived mesenchymal stem cells proliferation, stemness, and reprogramming into induced pluripotent stem cells

Deng

Huang

Chen

et al. 2019

Journal Cellular Physiology

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Adipose tissue‐derived mesenchymal stem cells (ASCs) from livestock are valuable resources for animal reproduction and veterinary therapeutics. Previous studies have shown that hypoxic conditions were beneficial in maintaining the physiological activities of ASCs. However, the effects of hypoxia on buffalo ASCs (bASCs) remain unclear. In this study, the effects of hypoxia on proliferation, stemness, and reprogramming into induced pluripotent stem cells (iPSCs) of bASCs were examined. The results showed that the hypoxic culture conditions (5% oxygen) enhanced the proliferation and colony formation of bASCs. The expression levels of proliferation‐related genes, and secretion of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) were significantly enhanced in hypoxia. Hypoxic culture conditions activated hypoxia‐inducible factor‐1α (HIF‐1α), thereby contributing to the secretion of bFGF and VEGF, which in turn enhanced the expression of HIF‐1α and promoted the proliferation of bASCs. Furthermore, in hypoxic culture conditions, bASCs exhibited the main characteristics of mesenchymal stem cells, and the expression levels of the pluripotent markers OCT4, NANOG, C‐MYC, and the differentiation capacity of bASCs were significantly enhanced. Finally, bASCs were more efficiently and easily reprogrammed into iPSCs in hypoxic culture conditions and these iPSCs exhibited some characteristics of naïve pluripotent stem cells. These findings provide the theoretical guidance for elucidating the detailed mechanism of hypoxia on physiological activities of bASCs including proliferation, stemness maintenance, and reprogramming.

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Section: Discussionmentioning

confidence: 99%

Section: Hypoxia Promotes Reprogramming Of Bascs Into Ipscsmentioning

confidence: 99%

mentioning

confidence: 99%

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Hypoxia enhances buffalo adipose‐derived mesenchymal stem cells proliferation, stemness, and reprogramming into induced pluripotent stem cells

Deng

Huang

Chen

et al. 2019

Journal Cellular Physiology

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Revolutionize livestock breeding in the future: an animal embryo-stem cell breeding system in a dish

Hou

Han

et al. 2018

J Animal Sci Biotechnol

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Meat and milk production needs to increase ~ 70–80% relative to its current levels for satisfying the human needs in 2050. However, it is impossible to achieve such genetic gain by conventional animal breeding systems. Based on recent advances with regard to in vitro induction of germ cell from pluripotent stem cells, herein we propose a novel embryo-stem cell breeding system. Distinct from the conventional breeding system in farm animals that involves selecting and mating individuals, the novel breeding system completes breeding cycles from parental to offspring embryos directly by selecting and mating embryos in a dish. In comparison to the conventional dairy breeding scheme, this system can rapidly achieve 30–40 times more genetic gain by significantly shortening generation interval and enhancing selection intensity. However, several major obstacles must be overcome before we can fully use this system in livestock breeding, which include derivation and mantaince of pluripotent stem cells in domestic animals, as well as in vitro induction of primordial germ cells, and subsequent haploid gametes. Thus, we also discuss the potential efforts needed in solving the obstacles for application this novel system, and elaborate on their groundbreaking potential in livestock breeding. This novel system would provide a revolutionary animal breeding system by offering an unprecedented opportunity for meeting the fast-growing meat and milk demand of humans.

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Basic fibroblast growth factor is critical to reprogramming buffalo ( Bubalus bubalis ) primordial germ cells into embryonic germ stem cell-like cells

Cited by 2 publications

References 47 publications

Hypoxia enhances buffalo adipose‐derived mesenchymal stem cells proliferation, stemness, and reprogramming into induced pluripotent stem cells

Hypoxia enhances buffalo adipose‐derived mesenchymal stem cells proliferation, stemness, and reprogramming into induced pluripotent stem cells

Revolutionize livestock breeding in the future: an animal embryo-stem cell breeding system in a dish

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