Basic aspects of implantation

Guzeloglu‐Kayisli, Ozlem; Başar, Murat; Arıcı, Aydın

doi:10.1016/s1472-6483(10)60541-x

Cited by 63 publications

(40 citation statements)

References 133 publications

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“…These results indicate that MTOR may participate in the proliferation of uterine stromal cells. During the process of implantation, a large number of molecular mediators were secreted by endometrium to support pregnancy, including cytokines, growth factors, lipids, and others (Achache & Revel 2006, Guzeloglu-Kayisli et al 2007. MTOR is a serine/ threonine protein kinase that has been shown to regulate cell growth in response to nutrients and growth factors (Thomas & Hall 1997).…”

Section: Discussionmentioning

confidence: 99%

The role of MTOR in mouse uterus during embryo implantation

He¹,

Ding²,

Zeng³

et al. 2009

Reproduction

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Mammalian target of rapamycin (MTOR) is a protein kinase that plays a central role in cell growth and proliferation. It is a part of the signaling network transmitting growth factor signaling to translational control. Previous studies have shown that MTOR is involved in embryo implantation, but its expression in the uterus and its role in implantation are unclear. Here, we have investigated the expression and role of MTOR in mouse uterus during early pregnancy. RT-FQ PCR showed that the mRNA levels of Mtor in endometria of pregnant mice were higher than those of nonpregnant mice. The mRNA levels in the pregnant mice gradually increased from D3 of pregnancy, reached maximum on D5, and then declined afterward. In situ hybridization and immunohistochemical analysis showed that the mRNA and protein of MTOR were mainly located in stromal cells. The levels of the expressed MTOR protein correlate with those of mRNA. The number of implantation sites was greatly decreased by the intrauterine injection with rapamycin in the early morning of D4 of the pregnancy. These findings suggest that MTOR may play an important role in embryo implantation in mice.

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Section: Discussionmentioning

confidence: 99%

The role of MTOR in mouse uterus during embryo implantation

He¹,

Ding²,

Zeng³

et al. 2009

Reproduction

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“…The implantation of an embryo in the uterus wall, which involves a complex series of interactions and events, is the first requirement for the embryo to develop beyond the blastocyst stage and progress to term (1). The successful establishment of endometrial receptivity and the subsequent differentiation of the endometrium into decidua are the key events in embryo implantation (2,3).…”

Section: Introductionmentioning

confidence: 99%

Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

Bahar¹,

Kahraman²,

Eras³

et al. 2015

Turk J Med Sci

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The aim of this study was to compare the cellular properties of endometrial tissues from fertile patients and patients having at least 3 previous in vitro fertilization failures, during the implantation window. The ultrastructural evaluation of the endometrium in the implantation window may shed light on the complexity of the implantation failure paradigm. Materials and methods:The study involved 23 women, 14 infertile with a clinical diagnosis of repeated implantation failure (RIF) and 9 fertile, defined as the control group. Endometrial samples were examined by transmission electron microscopy (TEM).Results: In the control group, secretory vacuoles and cytoplasmic projections filled with secretory material, called pinopodes, were noted; microvilli were observed on some apical surfaces; and ciliated cells were absent. In the RIF group, the number of pinopodes was remarkably lower, with some of them being immature. Moreover, decidualization of stromal cells was not frequent and fewer epithelial cells with poor secretory vacuoles were discerned. Conclusion:TEM analyses of endometrial samples from the RIF group revealed dramatic differences at the ultrastructural level compared to the controls, which may well be an underlying cause of their infertility.

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“…While some of the implantation failures might be attributable to a faulty embryo, even with the use of Preimplantation Genetic Diagnostic techniques, the implantation rate remains quite poor [24]. Numerous factors have been hailed as markers of the implantation window, with most of them turning out to be redundant for the receptivity period in humans [3]. However, by careful elimination of such factors, we are getting closer to pinpointing the true determinants of receptivity with possible future treatments aimed at improving this stage in human reproduction.…”

Section: Discussionmentioning

confidence: 99%

“…While we have methods to describe the development of an embryo either by morphologic indices or genetic screening, a proper description of endometrial receptiveness still eludes us [1,2]. There are many potential markers of the so-called 'implantation window' but most of them have proven to be redundant in the implantation processes [3]. The search for markers had been chaotic at best, until the introduction of gene chip matrices that allow simultaneous analysis of many thousands of genes.…”

Section: Introductionmentioning

confidence: 99%

No correlation between pinopode formation and LIF and MMP2 expression in endometrium during implantation window

Mikołajczyk¹,

Skrzypczak²,

Wirstlein³

2012

Folia Histochem Cytobiol.

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Implantation depends on two factors -embryo and endometrium. The period of maximal endometrial receptivity is a poorly understood phenomenon. We decided to look at three possible markers of implantation: pinopodes, leukemia inhibitory factor, and matrix metalloproteinase 2 and their correlations. We included in the study 23 idiopathic infertility patients and 21 patients with recurrent spontaneous abortions of unknown etiology. Twenty one fertile patients were also recruited. A biopsy was used for endometrial dating according to the Noyes and Hertig criteria, and assessed for the presence of pinopodes via a scanning electron microscope. Endometria were examined in Real Time-Polymerase Chain Reaction cycles for the mRNA expression of leukemia inhibitory factor (LIF) and matrix metalloproteinase 2 (MMP2). No difference was found in the stage of pinopodes development, nor in the coverage of endometrial surface between the studied groups. The expression level for LIF mRNA was lower in control patients compared to idiopathic infertility and recurrent miscarriage patients. No difference was detected in the expression of MMP2 between all studied groups. No correlation was found between pinopodes development stage and LIF and MMP2 expressions in endometrium. Of the studied factors, LIF and pinopodes show the most promise as potential markers of endometrial receptivity. However, the results achieved suggest that these markers are independent

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Basic aspects of implantation

Cited by 63 publications

References 133 publications

The role of MTOR in mouse uterus during embryo implantation

The role of MTOR in mouse uterus during embryo implantation

Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

No correlation between pinopode formation and LIF and MMP2 expression in endometrium during implantation window

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