Baseline susceptibility of Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae) in Australia to spirotetramat, cyantraniliprole and dinotefuran, with reference to pyriproxyfen cross‐resistance

Abstract

Silverleaf whitefly, Bemisia tabaci (Gennadius) Middle East‐Asia Minor 1 (MEAM1), previously known as B biotype, is an agricultural pest of global significance and has developed resistance to many commonly used insecticides. Toxicity of spirotetramat, cyantraniliprole and dinotefuran to B. tabaci MEAM1 populations from eastern Australia was determined by laboratory bioassay, using either a foliar leaf dip or systemic uptake methodology. All field‐collected populations tested were susceptible to spirotetramat (12 tested from 2011 to 2015), cyantraniliprole (23 tested from 2014 to 2017) and dinotefuran (16 tested from 2015 to 2017). Based on LC50 values, there was a 2.1‐fold difference in the response of field populations to spirotetramat, a threefold difference in response to cyantraniliprole, while the response to dinotefuran varied depending on assay used, 1.8 vs. 2.6 with a foliar assay and systemic uptake assay, respectively. Testing of spirotetramat and cyantraniliprole against a pyriproxyfen‐resistant strain, AY09‐1R demonstrated no evidence of cross‐resistance to pyriproxyfen. When tested against dinotefuran, AY09‐1R had a resistance factor of two indicating possible weak cross‐resistance to pyriproxyfen, although it is also possible that the strain carries other forms of resistance given its origin. Discriminating doses of 100 mg/L for spirotetramat, 1 mg/L for cyantraniliprole and 60 mg/L (systemic) and 600 mg/L (foliar) for dinotefuran were calculated from the bioassay data. All three insecticides demonstrated good efficacy against silverleaf whitefly, and for the Australian cotton industry, they add three modes of action to the insecticide resistance management strategy, providing greater flexibility in control options. This study has established baseline toxicity data and discriminating doses for spirotetramat, cyantraniliprole and dinotefuran, which will serve as a useful reference for future monitoring and management of B. tabaci MEAM1 insecticide resistance in Australian cotton.

Antalya (Türkiye)’dan Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae) popülasyonlarının cyantraniliprole, pyriproxyfen ve spirotetramata direnç düzeyleri

“…Other research (mentioned below) partially overlap with our study's low LC50 and resistance ratios. Hopkinson & Pumpa (2019) reported the susceptibility status of B. tabaci populations to spirotetramat ranged from 2.80 to 5.98 mg a.i./l with a 2.1 times difference. Prabhaker et al (2014) determined the susceptibility status of B. tabaci in Arizona and California to spirotetramat.…”

“…There were studies conducted on determination of the resistance of B. tabaci to cyantraniliprole, spirotetramat and pyriproxyfen in a number of countries. Hopkinson & Pumpa (2019) studied the toxicity of spirotetramat, cyantraniliprole and dinotefuran to Australian B. tabaci MEAM1 populations. Gravalos et al (2015) investigated the resistance and cross-resistance of B. tabaci Mediterranean strains collected from Greece, Italy and Spain to cyantraniliprole.…”

Extraction and determination of flubendiamide insecticide in food samples: A review

“…However, the researcher continued to pursue a wide range of polar groups on the anthranilic core with an emphasis on nitrile substitution ( Barrania, 2019 ; Bolzan et al, 2019 ; Carscallen et al, 2019 ; Mao et al, 2019 ; Gao et al, 2019 ; Meng et al, 2019 ; O'Neal et al, 2019 ; Sharma et al, 2019 ; Sreedhar, 2019 ; Truong and Pessah, 2019 ; Jiang et al, 2020 ). This effort resulted in the discovery of cyantraniliprole, a second product candidate in this chemical class possessing excellent interspectral activity against a range of insect orders, including lepidopteran and hemipteran pests ( Hopkinson and Pumpa, 2019 ; Plata-Rueda et al, 2019 ; Ran et al, 2019 ; Zhang et al, 2019 ). This insecticide product exhibits a very low toxicity to vertebrates and non-target organisms.…”

Empowering Australian insecticide resistance research with genetic information: the road ahead

“…B. tabaci is a major pest of Australian vegetable crops and cotton (Gunning 1995; Subramaniam 2010; Hall et al . 2012; Hopkinson & Pumpa 2019) and a minor pest of summer grains and oilseed crops (Sequeira et al . 2009).…”

“…In contrast, all other species have cosmopolitan distributions. B. tabaci is a major pest of Australian vegetable crops and cotton (Gunning 1995;Subramaniam 2010;Hall et al 2012;Hopkinson & Pumpa 2019) and a minor pest of summer grains and oilseed crops (Sequeira et al 2009). F. occidentalis is a pest in vegetable, ornamental, cotton, fruit and legume crops in Australia (Herron & Gullick 2001 (Kay 1977;Gunning et al 1984;Glenn et al 1994;Bird 2015;Downes et al 2016;Bird et al 2017).…”