Baseline and salt‐stimulated paraoxonase and arylesterase activities in patients with chronic liver disease: relation to disease severity

Keskin, Murat; Dolar, Enver; Dirican, Melahat; Kıyıcı, Murat; Yılmaz, Yusuf; Gürel, Selım; Nak, Selim Giray; Erdinç, Selda; Gülten, Macit

doi:10.1111/j.1445-5994.2009.01793.x

Cited by 25 publications

(16 citation statements)

References 19 publications

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“…To date, similar evaluation of POase and DZOase activities as diagnostic tests for sepsis was not performed. However, measurement of PON1 arylesterase activity was found to be an efficient test for identifying the presence and severity of chronic liver injury [6]. …”

Section: Discussionmentioning

confidence: 99%

“…Growing interest in the enzyme's importance is prompted by its role in lipid peroxidation and the development of atherosclerosis [5]. Decreased PON1 activity was also observed in liver disease [6, 7], acute pancreatitis [8], diabetes [9], chronic renal failure, and dialysis [10, 11]. So far, no data have been reported on the utility of serial PON1 measurement in surgical patients with sepsis.…”

Section: Introductionmentioning

confidence: 99%

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Low Paraoxonase 1 Activity Predicts Mortality in Surgical Patients with Sepsis

et al. 2014

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Introduction. State of severe oxidative stress is encountered in sepsis. Paraoxonase 1 (PON1) protects against oxidative stress but also undergoes inactivation upon that condition. We investigated PON1 activity in surgical patients with sepsis in relation to oxidative stress status, inflammation, disease severity, and survival. Methods. Prospective observational study. Sixty-nine surgical patients with sepsis were compared to 69 age/sex matched healthy controls. PON1 paraoxonase and diazoxonase activities, selected biochemical, hematological and oxidative stress parameters were measured on admission to ICU and 24, 48, 72, and 96 hours later. Disease severity scores were calculated daily. Results. Septic patients had significantly lower PON1 activities compared to control group at all time points. PON1 activities had good capacity to differentiate septic patients from healthy controls. Low PON1 activities were associated with higher disease severity scores and higher risk of death. Correlation between PON1 activity and markers of inflammation failed to reach significance. Decrease in PON1 activity was correlated with an increase in reducing components in plasma. Conclusion. Our study demonstrated lower PON1 activity in surgical patients with sepsis compared to healthy controls. PON1 activity also reflected severity of the disease. Low PON1 activity was associated with higher mortality of surgical patients with sepsis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low Paraoxonase 1 Activity Predicts Mortality in Surgical Patients with Sepsis

et al. 2014

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“…PON1 enzyme levels can range widely even between individuals with the same PON1 phenotype [34] and PON1 phenotypes do not influence changes in PON1 associated with some clinical conditions such as hepatic disease [27,35]. However in substrates highly influenced by polymorphisms, such as paraoxon in humans, there is a potential for obtaining false high values of PON1 in populations with high frequency of PON1R when compared with populations with high frequency of PON1Q and vice versa [4].…”

Section: Characteristics Of the Substratesmentioning

confidence: 99%

Serum paraoxonase 1 (PON1) measurement: an update

2014

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Paraoxonase 1 (PON1) is a widely studied enzyme based on its protective role against poisoning by organophosphate (OP) metabolites of specific OP insecticides and in vascular disease, as well as its use as biomarker of diseases involving oxidative stress, inflammation and liver diseases.This review provides an update about the current knowledge in the field of the analytical procedures that are used for PON1 measurements. It will be specially focused on: (a) characteristics of the different substrates used for measuring PON1, with emphasis in four aspects: toxicity, polymorphism influence, rate of hydrolysis and diagnostic performance. And (b) the technical aspects of PON1 assays, in which the reagents and reaction conditions, sources of variation, quality control systems, equipment and interferences with other esterases will be discussed.The information provided in this review can contribute to a more accurate and safe measurements of PON1 in laboratories and encourage researchers to explore the wide areas of PON1 in veterinary medicine that are still unknown.

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“…However, use of paraoxon as substrate would not be optimal due to its toxicity (Camps et al, 2009) and the possibility of analyzer contamination (Mogarekar and Chawhan, 2013). Divergences in the diagnostic performance between different substrates have been described in certain diseases (Dantoine et al, 1998;Keskin et al, 2009). Therefore, comparative studies in which various substrates are used for PON1 measurements would be recommended when this enzyme is evaluated in a new disease.…”

Section: Introductionmentioning

confidence: 99%

Serum biomarkers of oxidative stress in cats with feline infectious peritonitis

Tecles

Caldín²,

Tvarijonaviciute

et al. 2015

Research in Veterinary Science

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The purpose of this study was to elucidate the possible presence of oxidative stress in cats naturally affected by feline infectious peritonitis (FIP) by investigating two antioxidant biomarkers in serum: paraoxonase-1 (PON1) and total antioxidant capacity (TAC). PON1 was measured by spectrophotometric assays using three different substrates: p-nitrophenyl acetate (pNA), phenyl acetate (PA) and 5-thiobutil butyrolactone (TBBL), in order to evaluate possible differences between them. The PA and TBBL assays for PON1 and the assay for TAC were validated, providing acceptable precision and linearity although PA and TAC assays showed limit of detection higher than the values found in some cats with FIP. Cats with FIP and other inflammatory conditions showed lower PON1 values compared with a group of healthy cats with the three assays used, and cats with FIP showed significant decreased TAC concentrations. This study demonstrated the existence of oxidative stress in cats with FIP.

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Baseline and salt‐stimulated paraoxonase and arylesterase activities in patients with chronic liver disease: relation to disease severity

Abstract: Baseline and stimulated PON1 and ARE activities are reduced in patients with chronic liver disease. Serum ARE activity could be a suitable biomarker for the evaluation of the presence and severity of chronic liver damage.

Cited by 25 publications

References 19 publications

Low Paraoxonase 1 Activity Predicts Mortality in Surgical Patients with Sepsis

Low Paraoxonase 1 Activity Predicts Mortality in Surgical Patients with Sepsis

Serum paraoxonase 1 (PON1) measurement: an update

Serum biomarkers of oxidative stress in cats with feline infectious peritonitis

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