Basal plasma concentrations of N-terminal pro-B-type natriuretic peptide in clinically healthy adult small size dogs: Effect of body weight, age, gender and breed, and reference intervals

Misbach, Charlotte; Chetboul, Valérie; Concordet, Didier; Gruet, Philippe; Speranza, C.; Hoffmann, A; Rocha, Adriana; Balouka, David; Petit, A; Tréhiou-Sechi, Emilie; Pouchelon, Jean‐Louis; Lefebvre, Henri

doi:10.1016/j.rvsc.2013.07.025

Cited by 13 publications

(24 citation statements)

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“…A minor tendency of variation between male and female rabbits for ANP in all breeds (except for Flemish Giant) was observed; elevated serum concentrations of ANP were observed in females, compared to males. These results were similar to those reported by Misbach et al (2013), who demonstrated that female dogs had an elevated concentration of plasmatic NT-pro-BNP, compared to male dogs. In our study, gender--specific differences in other cardiac markers were either not observed (ACE) or were observed on a smaller scale (CRP).…”

Section: Discussionsupporting

confidence: 92%

“…The authors reported significant differences in plasma NT-proBNP concentration in seven different breeds. The study concluded that breed--specific reference ranges might, therefore, be necessary for optimal clinical use of natriuretic peptides as cardiac biomarkers (Misbach et al 2013). Results of the present study corroborate the results of this study.…”

Section: Discussionsupporting

confidence: 89%

“…More recently, Misbach et al (2013) investigated the effect of breed on basal plasma concentrations of NT-pro-BNP in clinically-healthy adult dogs. The authors reported significant differences in plasma NT-proBNP concentration in seven different breeds.…”

Section: Discussionmentioning

confidence: 99%

“…In another study, Höllmer et al (2013) investigated the effects of breed variability on left atrial volume and phasic function in clinically healthy dogs. In a recently published paper, Misbach et al (2013) investigated the effects of breed variability (including age, gender, and body) on basal plasma concentrations of the cardiac marker, N-terminal pro-B-type natriuretic peptide (NT-proBNP), in clinically healthy adult dogs. In the above study, the authors observed marked differences in plasma levels of NT-proBNP in seven different breeds of dogs.…”

Section: Introductionmentioning

confidence: 99%

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Cardiac markers in five different breeds of rabbits (Oryctolagus cuniculus Linnaeus, 1758) used for cardiovascular research

Ferreira¹,

Barretto²,

Fabres³

et al. 2016

Pesq. Vet. Bras.

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RESUMO.-[Marcadores cardíacos em cinco diferentes raças de coelhos (Oryctolagus cuniculusLinnaeus, 1758) utilizados para pesquisa cardiovascular.] Biomarcadores cardíacos têm sido avaliados em coelhos para avaliação clínica e experimental das doenças cardíacas. Entretanto diferentes testes laboratoriais têm sido utilizados e relatados, sem uma confluência de resultados. Os objetivos deste estudo foram verificar os efeitos de diferentes raças de coelhos sobre as concentrações séricas de ANP, CRP e ACE, além de estabelecer intervalor de referência para estes biomarcadores em uma população de coelhos saudáveis. Foram utilizados noventa e sete coelhos de cinco diferentes raças. Os exames foram realizados pela metodologia de ELISA, por meio de kits comerciais específicos. Os resultados foram analisados estatisticamente os testes de ANOVA e Tukey (p<0,05), média aritmética, intervalo de referência da média e desvio padrão. Um efeito significativo da raça foi observado sobre as variáveis estudadas, indicando diferentes intervalos de referência entre as raças para cada biomarcador. Em conclusão, este estudo demonstrou que a raça é uma variável fisiológica importante que influencia os valores normais destes biomarcadores em coelhos saudáveis.TERMOS DE INDEXAÇÃO: Enzima conversora de angiotensina, peptídeo natriurético atrial, proteína C reativa. INTRODUCTIONRabbits are widely used as laboratory animals for biomedical and cardiovascular research involving biomarkers of disease (Campbell et al. 1978, Yu et al. 1979, Barret et al. 2002, Chorro et al. 2009, Dontas et al. 2011, Özkan et al. 2012. Similarity of cardiac physiology (with humans), docility, easy handling, and adequate physical bearing of rab- Cardiac biomarkers for clinical and experimental heart diseases have previously been evaluated in rabbits. However, several laboratory assays performed and reported with inconsistent results. This study aimed to assess the effects of breed on serum ANP, CRP, and ACE and establish reference interval (RI) for these biomarkers in a large population of healthy rabbits. Ninety-seven adult rabbits from five breeds were included in this study. Assays were performed using specific ELISA commercial kits. The results were statistically analyzed using ANOVA, Tukey test (p<0.05), arithmetic mean, RI of mean, and standard deviation. A significant effect of breed was shown, indicating different RI between breeds for each biomarker. In conclusion, this study demonstrated that breed is an important physiological variable influencing the normal values of cardiac markers in healthy rabbits. Cardiac markers in five different breeds of rabbitsINDEX TERMS: Angiotensin-converting enzyme, atrial natriuretic peptide, breed, C-reactive protein.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cardiac markers in five different breeds of rabbits (Oryctolagus cuniculus Linnaeus, 1758) used for cardiovascular research

Ferreira¹,

Barretto²,

Fabres³

et al. 2016

Pesq. Vet. Bras.

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“…The role of the breed in canine NT‐proBNP plasma concentrations has been described previously, so it is possible that the higher NT‐proBNP range in Greyhounds represents just another breed‐related finding in healthy dogs, similar to Labradors and Newfoundlands . Therefore, given the high prevalence of physiologic murmurs, radiographic evidence of “cardiomegaly”, and “stiff” ventricles on echocardiography, NT‐proBNP values in Greyhounds should not be overinterpreted by the clinician.…”

mentioning

confidence: 99%

Plasma N‐terminal pro‐B‐type natriuretic peptide concentration in healthy retired racing Greyhounds

Couto

Iazbik

Marín

et al. 2015

Veterinary Clinical Pathol

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Pulmonary Vein‐to‐Pulmonary Artery Ratio is an Echocardiographic Index of Congestive Heart Failure in Dogs with Degenerative Mitral Valve Disease

Merveille

Bolen

Krafft

et al. 2015

Veterinary Internal Medicne

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BackgroundEarly recognition of left‐sided congestive heart failure (CHF) in dogs with degenerative mitral valve disease (DMVD) is important because it influences medical therapy, timing of follow‐up, and outcome.HypothesisPulmonary vein diameter‐to‐pulmonary artery diameter ratio (PV/PA) measured by echocardiography can predict CHF.AnimalsNinety‐eight client‐owned dogs, 37 controls, and 61 dogs with DMVD.MethodsProspective clinical cohort study. History, physical examination and Doppler‐echocardiography were performed. Dogs were classified as International Small Animal Cardiac Health Council class I, II or III. Congestive heart failure was identified in a subset of 56 dogs based on radiographic findings. The PV/PA was measured in bidimensional (2D) and M‐mode by 2 investigators blinded to the radiologists’ conclusions.ResultsInterobserver coefficients of variation for PV/PA acquisition and measurement were <10%. The PV/PA in control dogs was approximately 1 and increased with class of heart failure. The presence of CHF could be best predicted by measuring PV/PA in 2D echocardiography (cut‐off, 1.7; area under the curve, 0.98; CI, 0.97–0.98; P < .001) with a sensitivity of 96% and a specificity of 91%.Conclusion and clinical importanceThe PV/PA is a simple and reproducible echocardiographic variable that increases with class of heart failure and may help discriminate dogs in CHF from asymptomatic dogs with DMVD. Additional studies are required to determine whether PV/PA might provide additional information in the integrated interpretation of Doppler‐echocardiographic indices of left ventricular filling pressures and could be used for rapid assessment of CHF in dogs in a critical care setting.

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Basal plasma concentrations of N-terminal pro-B-type natriuretic peptide in clinically healthy adult small size dogs: Effect of body weight, age, gender and breed, and reference intervals

Cited by 13 publications

References 37 publications

Cardiac markers in five different breeds of rabbits (Oryctolagus cuniculus Linnaeus, 1758) used for cardiovascular research

Cardiac markers in five different breeds of rabbits (Oryctolagus cuniculus Linnaeus, 1758) used for cardiovascular research

Plasma N‐terminal pro‐B‐type natriuretic peptide concentration in healthy retired racing Greyhounds

Pulmonary Vein‐to‐Pulmonary Artery Ratio is an Echocardiographic Index of Congestive Heart Failure in Dogs with Degenerative Mitral Valve Disease

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