Cardiac markers in five different breeds of rabbits (Oryctolagus cuniculus Linnaeus, 1758) used for cardiovascular research

Abstract

RESUMO.-[Marcadores cardíacos em cinco diferentes raças de coelhos (Oryctolagus cuniculusLinnaeus, 1758) utilizados para pesquisa cardiovascular.] Biomarcadores cardíacos têm sido avaliados em coelhos para avaliação clínica e experimental das doenças cardíacas. Entretanto diferentes testes laboratoriais têm sido utilizados e relatados, sem uma confluência de resultados. Os objetivos deste estudo foram verificar os efeitos de diferentes raças de coelhos sobre as concentrações séricas de ANP, CRP e ACE, além de estabelecer intervalor de referência para estes biomarcadores em uma população de coelhos saudáveis. Foram utilizados noventa e sete coelhos de cinco diferentes raças. Os exames foram realizados pela metodologia de ELISA, por meio de kits comerciais específicos. Os resultados foram analisados estatisticamente os testes de ANOVA e Tukey (p<0,05), média aritmética, intervalo de referência da média e desvio padrão. Um efeito significativo da raça foi observado sobre as variáveis estudadas, indicando diferentes intervalos de referência entre as raças para cada biomarcador. Em conclusão, este estudo demonstrou que a raça é uma variável fisiológica importante que influencia os valores normais destes biomarcadores em coelhos saudáveis.TERMOS DE INDEXAÇÃO: Enzima conversora de angiotensina, peptídeo natriurético atrial, proteína C reativa. INTRODUCTIONRabbits are widely used as laboratory animals for biomedical and cardiovascular research involving biomarkers of disease (Campbell et al. 1978, Yu et al. 1979, Barret et al. 2002, Chorro et al. 2009, Dontas et al. 2011, Özkan et al. 2012. Similarity of cardiac physiology (with humans), docility, easy handling, and adequate physical bearing of rab- Cardiac biomarkers for clinical and experimental heart diseases have previously been evaluated in rabbits. However, several laboratory assays performed and reported with inconsistent results. This study aimed to assess the effects of breed on serum ANP, CRP, and ACE and establish reference interval (RI) for these biomarkers in a large population of healthy rabbits. Ninety-seven adult rabbits from five breeds were included in this study. Assays were performed using specific ELISA commercial kits. The results were statistically analyzed using ANOVA, Tukey test (p<0.05), arithmetic mean, RI of mean, and standard deviation. A significant effect of breed was shown, indicating different RI between breeds for each biomarker. In conclusion, this study demonstrated that breed is an important physiological variable influencing the normal values of cardiac markers in healthy rabbits. Cardiac markers in five different breeds of rabbitsINDEX TERMS: Angiotensin-converting enzyme, atrial natriuretic peptide, breed, C-reactive protein.

Development of a large animal rabbit model for chronic periprosthetic joint infection

“…All animals in the control group had normal ESR values and slightly elevated CRP values that were significantly lower than osteomyelitis values. 33 , 34 Taken together with the body mass and temperature profiles, these data indicate an early acute phase of the infection, lasting approximately 14 days, followed by a less symptomatic chronic phase. Laboratory values of WBC differentials revealed leukocytosis in the chronic PJI group.…”

Biomarcadores Cardíacos E Sua Aplicabilidade Clínica Em Cães E Gatos

“…Há grandes progressos nos estudos sobre os biomarcadores cardíacos, entretanto, não existe atualmente nenhum marcador cardíaco que tenha ganhado lugar no perfil bioquímico da rotina veterinária (ALVES et al, 2015;BOER et al, 2015;FERREIRA et al, 2016;MATTIN et al, 2018), embora os estudos já mostrem os benefícios da sua aplicação clínica na avaliação de pacientes graves, com ou sem doença cardíaca. Atualmente, a avaliação cardíaca na veterinária ainda se faz por meios de ausculta, radiografias, eletrocardiograma (ECG) e ecodopplercardiografia (ECO) (LANGHORN; WILLESEN, 2016; MILLER; JAFFE 2016).…”

N-terminal-pro brain natriuretic peptides in dogs and cats: A technical and clinical review

“…Most importantly, it is necessary to delay degradation (like hemolysis, for instance) during collection procedures and transportation to a laboratory. Furthermore, samples have to be centrifuged and kept under refrigeration immediately after collection [ 5 , 9 , 31 , 34 - 37 ]. Sample storage methods also greatly influence the values of NT-proBNP in serum.…”

“…Research has shown that serum and plasma levels of NT-proBNP in dogs and cats are the only biomarkers that afford to diagnose and monitor congestive processes and, indirectly, assess the myocardial function of small animals [ 2 ]. For this reason, the interest in NT-proBNP in veterinary medicine has grown in the past 10 years, even though it is used mainly in some research contexts because proper methodologies have not been validated and standardized for clinical practice [ 7 - 9 ].…”