Basal Anxiety Negatively Correlates with Vulnerability to Ethanol‐Induced Behavioral Sensitization in DBA/2J Mice: Modulation by Diazepam

Botia, BÃ©atrice; Legastelois, RÃ©mi; Houchi, Hakim; Naassila, Mickaël

doi:10.1111/acer.12595

Cited by 11 publications

(19 citation statements)

References 55 publications

(122 reference statements)

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“…Ethanol has both stimulatory and sedating effects depending on the dose administered. At low doses, ethanol increases locomotor activity, whereas higher doses reduce locomotor activity and can lead to loss of consciousness (June et al, 2003 ; Zapata et al, 2006 ; Kawakami et al, 2007 ; Arias et al, 2012 ; Kippin, 2014 ; Botia et al, 2015 ; Karlsson and Roman, 2016 ). As such, the more rapidly ethanol is consumed and the greater the intake, the more sedated the rat is likely to become, and the length of time the rat would be capable of engaging in the further consumption of ethanol would be reduced.…”

Section: Discussionmentioning

confidence: 99%

A Rat Drinking in the Dark Model for Studying Ethanol and Sucrose Consumption

Holgate

Shariff

et al. 2017

Front. Behav. Neurosci.

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Background: The intermittent access 2-bottle choice (IA2BC) and drinking in the dark (DID) models were developed for studying rodent binge-like consumption. Traditionally, IA2BC was used with rats and DID with mice. Recently, IA2BC was adapted to study mouse ethanol consumption. However, it is unknown whether DID is suitable for rats or if one rat model is more advantageous than another for studying binge-like consumption.Methods: Male Wistar rats consumed 20% ethanol or 5% sucrose using IA2BC or DID for 12 weeks. IA2BC drinking sessions occurred on alternate days (Mondays–Fridays) and lasted 24 h, whereas DID sessions ran 4 h/day, 5 days/week (Monday–Friday). Average consumption/session, week and hour was measured. To explore DID model suitability for screening novel compounds for controlling ethanol and sucrose intake, varenicline (2 mg/kg) or vehicle was administered to DID rats.Results: IA2BC rats consume more ethanol/session and similar amounts of ethanol/week than DID rats. While, IA2BC rats consume more sucrose/session and week than DID rats. Although IA2BC rats had more ethanol and sucrose access time, DID rats had greater ethanol and sucrose intake/hour. Varenicline significantly reduced ethanol and sucrose consumption in DID rats, consistent with previously published IA2BC studies.Conclusions: Despite the shorter access time, the rat DID model induced higher initial intake and greater consumption/hour for both ethanol and sucrose. The shorter duration of DID sessions did not prevent detection of varenicline-induced reductions in ethanol or sucrose consumption, suggesting the DID model may be suitable for studying binge-like ethanol and sucrose consumption.

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Section: Discussionmentioning

confidence: 99%

A Rat Drinking in the Dark Model for Studying Ethanol and Sucrose Consumption

Holgate

Shariff

et al. 2017

Front. Behav. Neurosci.

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“…During EtOH‐induced behavioral sensitization (EIBS) paradigm in mice, one robust result is the variability in the rodent's response, with some animals being sensitized at the end of the procedure and others not showing sensitization (called resistant). Although most of the literature on EtOH‐induced behavioral sensitization was dedicated to the mesolimbic circuitry, it has been shown that the dorsal hippocampus may endure some changes after EtOH‐ or other drugs of abuse‐induced sensitization (Degoulet et al, ; Botia et al, ; Coelhoso et al, ; Soares‐Simi et al, ; Legastelois et al, ; Nona et al, ). Different targets have been identified, such as the transcription factor CREB (Soares‐Simi et al, ; Nona et al, ), cannabinoid receptor expression (Coelhoso et al, ), low level of chromatin remodeling (Botia et al, ), BDNF and trkB receptor mRNA expression (Nona et al, ).…”

Section: Introductionmentioning

confidence: 99%

Resistance to ethanol sensitization is associated with a loss of synaptic plasticity in the hippocampus

Coune

Ferron

González-Marín

et al. 2016

Synapse

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Behavioral sensitization to repeated ethanol (EtOH) exposure induces an increase in locomotor activity in mice. However, not all animals express such sensitization. Although the literature indicated that the hippocampus may play a role in EtOH sensitization, it is not known whether behavioral sensitization to EtOH is associated with preferential changes in bidirectional synaptic plasticity, i.e., LTP and LTD, two markers of learning capabilities that have also been shown to be involved in addictive behavior. In the present study, we examined whether the vulnerability to develop and express behavioral sensitization to EtOH is associated with altered bidirectional synaptic plasticity in the CA1 area of the dorsal hippocampus. For this purpose, we analyzed both LTP and LTD in resistant and sensitized mice during the expression phase, i.e., 7 days after 10 days of repeated EtOH i.p. administration. We found that resistant mice showed a lack of LTD without changes in LTP. The lack of LTD was associated with an increase in GluN2A protein level and was not due to an altered level of neuronal activity, since no difference was observed between the number of c-FOS positive neurons in sensitized and resistant mice. Given that both types of synaptic plasticity signals may have distinct roles in specific learning and behaviors, our results suggest that resistant mice could exhibit different phenotypes in terms of learning/memory and addictive behaviors compared to sensitized ones. Synapse 71:e21899, 2017. © 2016 Wiley Periodicals, Inc.

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“…Mice were housed in groups of 10 in clear plastic cages (24 × 42 ×15 cm) and kept in a temperature (21 ± 0.5°C) and humidity-controlled (55 ± 10%) environment under an established photoperiod (07.00 -19.00 hours) with free access to food (Mouse and Rats, Maintenance, Extrudate; Provimi Kliba, Kaiseraugst, Switzerland) and tap water. In the present study, we used female mice in continuity with our previous work [13,[35][36][37][38][39][40][41] and also to keep consistency with the most relevant reports supporting our working hypothesis [37,39]. The number of animals was kept to a minimum, and all efforts were made to limit stress and to avoid animal suffering.…”

Section: Subjectsmentioning

confidence: 99%

“…This dual approach would eventually strengthen the accuracy of our results. The first sensitization score, hereafter mentioned as the "Delta Score" was calculated as [(locomotor activity on day 10) -(locomotor activity on day 1)] [13,41,43]. The second score, or "D10 Score", was the distance travelled during the 10th and last sensitization session [44,45].…”

Section: Eibs Proceduresmentioning

confidence: 99%

Sensitization to the Stimulant Motor Effects of Ethanol Is Not Dependent On Tolerance to Ataxic or Sedative Properties of Ethanol in Female Mice

Legastelois¹

2015

J Alcohol Drug Depend

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Ethanol (EtOH)-induced behavioral sensitization (EIBS) is defined as an enhancement of locomotor activity following repeated EtOH exposure and is proposed to reflect an increase in EtOH "wanting". However, the reliability of the sensitization model in studying addiction is still a matter of debate. One major criticism is that the increase in locomotion occurring during sensitization may be a by-product of tolerance to the ataxic and/or sedative effects of EtOH.We investigated the relationship between EIBS amplitude and sensitivity to EtOH-induced ataxia and sedation after the development of tolerance to EtOH depressant effects in adult female DBA/2J mice. After receiving daily injection of saline or 2 g/kg EtOH during 10 consecutive days to induce EIBS, recovery from acute motor incoordination produced by ethanol (2 g/kg EtOH using rotarod) and from loss of righting reflex (4 g/kg EtOH) was measured.We showed that induction of EtOH sensitization after repeated administration of EtOH is associated with a more rapid recovery from acute motor incoordination and from sedation produced by ethanol when compared to the acute groups, suggesting the development of tolerance to the ataxic and sedative effects of EtOH. However, correlational analyses failed to detect any relationship between EIBS amplitude and the response to EtOH ataxic or sedative effects.Altogether, our results confirm and extend previous data showing a tolerance to the ataxic and sedative properties of EtOH after repeated exposure to EtOH and suggest that this tolerance is not related to the amplitude of EIBS.

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Basal Anxiety Negatively Correlates with Vulnerability to Ethanol‐Induced Behavioral Sensitization in DBA/2J Mice: Modulation by Diazepam

Cited by 11 publications

References 55 publications

A Rat Drinking in the Dark Model for Studying Ethanol and Sucrose Consumption

A Rat Drinking in the Dark Model for Studying Ethanol and Sucrose Consumption

Resistance to ethanol sensitization is associated with a loss of synaptic plasticity in the hippocampus

Sensitization to the Stimulant Motor Effects of Ethanol Is Not Dependent On Tolerance to Ataxic or Sedative Properties of Ethanol in Female Mice

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