Bartonella machadoae sp. nov. isolated from wild rodents in the Pantanal wetland

Molecular detection of vector‐borne agents in wild boars ( Sus scrofa ) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia , Anaplasma , and haemoplasmas

“…berkhoffi (three sequences) in 19.7% (15/76) of feral pigs in North Carolina, USA. Future studies should be conducted aiming at submitting wild boars’ blood samples to the diagnostic platform that combines BAPGM liquid cultures ( Bartonella Alpha Proteobacteria Growth Medium) and solid cultures on blood or chocolate agar, followed by real‐time and conventional PCR assays, in order to increase the chances of detection of Bartonella spp., as recently demonstrated in blood samples from cats from south‐eastern Brazil (Furquim et al., 2021) and rodents from central‐western Brazil (do Amaral et al., 2022).…”

“…In contrast, 3.80% (7/184) of the ticks collected from wild boars in the present study were positive for Bartonella spp. The phylogenetic inferences based on both nuoG and gltA genes positioned the sequences obtained from A. sculptum collected from wild boars in the same clade as B. machadoae , a species recently described by our research group in wild rodents in the Brazilian Pantanal (do Amaral et al., 2022), with a clade support of 95% and 55%, respectively. To the best of authors’ knowledge, this is the first report of Bartonella sp.…”

“…Growth Medium) and solid cultures on blood or chocolate agar, followed by real-time and conventional PCR assays, in order to increase the chances of detection of Bartonella spp., as recently demonstrated in blood samples from cats from south-eastern Brazil(Furquim et al, 2021) and rodents from central-western Brazil (doAmaral et al, 2022).In contrast, 3.80% (7/184) of the ticks collected from wild boars in the present study were positive for adults]) of I. ricinus ticks collected from vegetation in France were positive for Bartonella spp. by a semi-nested PCR assay based on the gltA gene.…”

Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguaçu National Park, southern Brazil

“…Five out of 12 qPCR-positive samples for Bartonella also showed positivity in a PCR assay based on the pap-31 gene. The obtained sequences ranged from 382 to 532 bp and showed 99.06–100% ID, 100% QC, 0.0 E-value and with Bartonella machadoae 37 . Seven out of 12 qPCR-positive samples for Bartonella also showed positivity in a PCR assay based on the gltA gene, with sequences ranging from 401 to 582 bp and showing 99.83–100% ID, 100% QC and 0.0 EC with Bartonella machadoae .…”

“…(16S rRNA gene) HemoF1 HemoR2 HemoF2 Screening snPCR 5′-AGAGTTTGATCCTGGCTCAG-3′ 5′-TACCTTGTTACGACTTAACT-3 5′-ATATTCCTACGGGAAGCAGC-3′ 1107 95 °C for 5 min, followed by 35 cycles of 95 °C for 30 s, 57 °C for 30 s, an extension at 72 °C for 1 min, and a final extension at 72 °C for 10 min, for both rounds 34 Hepatozoon spp. (18S rRNA) HAM-1 HPF-2 4558 2733 Screening nPCR 5′ GCCAGTAGT CATATGCTTGTC 3′ 5′ GACTTCTCCTTCGTCTAAG 3′ 5′ GCTAATACATGAGCAAAA TCTCAA 3′ 5′ CGGAATTAACCAGACAAAT 3′ 1120 95 °C for 3 min, followed by 40 cycles of 95 °C for 1 min, 56 °C for 1 min, an extension at 72 °C for 1 min and 30 s, and a final extension at 72 °C for 7 min 35 , 36 Piroplasmids (18S rRNA gene) BTF1 BTR1 BTF2 BTR2 Screening nPCR 5′-GGCTCATTACAACAGTTATAG-3′ 5′-CCCAAAGACTTTGATTTCTCTC-3′ 5′-CCGTGCTAATTGTAGGGCTAATAC-3′ 5′-GGACTACGACGGTATCTGATCG-3′ 800 94 °C for 3 min, followed by 45 cycles of 94 °C for 30 seg, 58 °C for 20 seg, 72 °C for 30 seg, and a final extension at 72 °C for 7 min 37 …”