Bartha-k61 vaccine protects growing pigs against challenge with an emerging variant pseudorabies virus

Zhou, Jinzhu; Li, Shuang; Wang, Xiaobo; Zou, Miaomiao; Gao, Song

doi:10.1016/j.vaccine.2017.01.003

Cited by 60 publications

(38 citation statements)

References 27 publications

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“…The current study demonstrated that sRNase L expression inhibited PRV replication in PK-15 cells ( Figure 5). Attenuated PRV vaccines, in particular the PRV Bartha-K61 strain, have been widely used by swine industry to control pseudorabies [14]. In this study, sRNase L KO-PK cells showed increased replication of PRV Bartha-K61 vaccine strain than parental PK-15 cells ( Figures 5(c), and 5(d) and Table 2).…”

Section: Discussionmentioning

confidence: 58%

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CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

Sui

Jiang

et al. 2019

BioMed Research International

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Ribonuclease L (RNase L) is an important antiviral endoribonuclease regulated by type I IFN. RNase L is activated by viral infection and dsRNA. Because the role of swine RNase L (sRNase L) is not fully understood, in this study, we generated a sRNase L knockout PK-15 (KO-PK) cell line through the CRISPR/Cas9 gene editing system to evaluate the function of sRNase L. After transfection with CRISPR-Cas9 followed by selection using puromycin, sRNase L knockout in PK-15 cells was further validated by agarose gel electrophoresis, DNA sequencing, and Western blotting. The sRNase L KO-PK cells failed to trigger RNA degradation and induced less apoptosis than the parental PK-15 cells after transfected with poly (I: C). Furthermore, the levels of ISGs mRNA in sRNase L KO-PK cells were higher than those in the parental PK-15 cells after treated with poly (I: C). Finally, both wild type and attenuated pseudorabies viruses (PRV) replicated more efficiently in sRNase L KO-PK cells than the parental PK-15 cells. Taken together, these findings suggest that sRNase L has multiple biological functions including cellular single-stranded RNA degradation, induction of apoptosis, downregulation of transcript levels of ISGs, and antiviral activity against PRV. The sRNase L KO-PK cell line will be a valuable tool for studying functions of sRNase L as well as for producing PRV attenuated vaccine.

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Section: Discussionmentioning

confidence: 58%

“…At present, the widely used PRV vaccine has played an important role in the control of pseudorabies (PR). The major vaccines are gE knockout strains, including Bartha-K61 strain [14].…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

Sui

Jiang

et al. 2019

BioMed Research International

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“…Humoral immune response contributes a vital role in the immune defense against PrV [48,49]. In mice, subclasses of IgG include IgG1, IgG2a, IgG2b, and IgG3.…”

Section: Discussionmentioning

confidence: 99%

A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice

Wang

Cui

et al. 2020

Journal of Immunology Research

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Pseudorabies is an important infectious disease of swine, and immunization using attenuated pseudorabies virus (aPrV) vaccine is a routine practice to control this disease in swine herds. This study was to evaluate a saline solution containing ginseng stem-leaf saponins (GSLS) and sodium selenite (Se) as a vaccine adjuvant for its enhancement of immune response to aPrV vaccine. The results showed that aPrV vaccine diluted with saline containing GSLS-Se (aP-GSe) induced significantly higher immune responses than that of the vaccine diluted with saline alone (aP-S). The aP-GSe promoted higher production of gB-specific IgG, IgG1, and IgG2a, neutralizing antibody titers, secretion of Th1-type (IFN-γ, IL-2, IL-12), and Th2-type (IL-4, IL-6, IL-10) cytokines, and upregulated the T-bet/GATA-3 mRNA expression when compared to aP-S. In addition, cytolytic activity of NK cells, lymphocyte proliferation, and CD4 + /CD8 + ratio was also significantly increased by aP-GSe. More importantly, aP-GSe conferred a much higher resistance of mice to a field virulent pseudorabies virus (fPrV) challenge. As the present study was conducted in mice, further study is required to evaluate the aP-GSe to improve the vaccination against PrV in swine.

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“…Each virus was harvested when a cytopathic effect of approximately 80% was observed. When complete cytopathic effect was observed, the plate was frozen and thawed three times, and cell debris was removed by centrifugation at 3000Âg for 10 min [22]. The clear supernatant fluid was used as the virus stock.…”

Section: Preparation Of Virus Suspensions For Titrationmentioning

confidence: 99%

Glutaraldehyde inactivation of enveloped DNA viruses in the preparation of haemoglobin-based oxygen carriers

Feng

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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Glutaraldehyde (GA), used medically as a disinfectant and as a crosslinker for haemoglobin (Hb)-based oxygen carriers (HBOCs), was investigated for its ability to inactivate viruses during the preparation of these artificial blood substitutes. Porcine parvovirus (PPV; a non-enveloped DNA virus) and porcine pseudorabies virus (PRV; an enveloped DNA virus) were used as the virus indicators. Upon treatment with 0.1 mM GA, the titer of PRV decreased from 9.62 log to 2.62 log within 0.5 h, whereas that of PPV decreased from 7.00 log to 2.30 log in 5 h. Following treatment with 1.0 mM GA, the titer of PRV decreased from 11.00 log to 1.97 log within 0.5 h, whereas that of PPV decreased from 7.50 log to 3.43 log in 4.5 h. During the polymerization of Hb with GA, the GA concentration decreased to 1.0 and 0.1 mM within 30 and 50 min, respectively, at a GA:Hb molar ratio of 10:1, whereas at a GA:Hb molar ratio of 30:1, GA decreased to those same concentrations in 1.5 and 2.5 h, respectively. This rapid decrease in GA concentration during its polymerization with Hb indicates that GA must be added into the Hb solution in a short time in order to get as high a initial concentration as possible. In this study, the GA can only inactivate PRV effectively, given that a longer time (4.5 h) was required for it to inactivate the PPV titer. This study therefore demonstrates that GA inactivates the enveloped DNA virus only during the preparation of HBOCs.

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Bartha-k61 vaccine protects growing pigs against challenge with an emerging variant pseudorabies virus

Cited by 60 publications

References 27 publications

CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

CRISPR-Cas9 Mediated RNase L Knockout Regulates Cellular Function of PK-15 Cells and Increases PRV Replication

A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice

Glutaraldehyde inactivation of enveloped DNA viruses in the preparation of haemoglobin-based oxygen carriers

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