Barrier‑to‑autointegration factor 1: A novel biomarker for gastric cancer

Abstract

China is a country with a high incidence of gastric cancer (GC), where the GC incidence and the resultant mortality rates account for 50% of those worldwide. Surgical resection remains the primary treatment for GC. However, postoperative patients have a poor prognosis as the majority of patients present with metastases at the time of diagnosis. Therefore, the identification of novel treatment targets is required. The present study aimed to determine the effects of barrier-to-autointegration factor 1 (BANF1) on the clinical features and prognosis of GC, which may aid in discovering a novel tumor diagnostic biomarker and treatment target. The BANF1 gene expression profiles for normal and gastric tumor tissues were downloaded from the Gene Expression Omnibus GSE54129 data set to analyse the expression of BANF1 at the mRNA levels. Then, online survival analysis was performed using the GC database with the Kaplan-Meier Plotter () data. To examine the association between BANF1 and clinical features and prognosis, 132 postoperative GC pathological specimens were collected for immunohistochemical analyses. In the GSE54129 data sets, BANF1 expression at the mRNA level was significantly higher in the tumor tissue compared with that in the normal tissue. The same result was obtained in following the immunohistochemical analyses. In addition, BANF1 expression was associated with the patient age, tumor differentiation and infiltration depth. The survival time of BANF1 high-expression patients was shorter compared with that of the low-expression patients, and tumor differentiation status and tumor node metastasis stage were independent prognostic factors of the overall survival of patients with GC. The results of the present study suggest that BANF1 is associated with the clinical features and prognosis of GC. It may be a novel indicator of tumor prognosis and a potential therapeutic target for GC.

Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells

“…These results revealed the essential role of BANF1 in regulating the mitotic process and normal cell cycle progression. Moreover, BANF1 has been reported to be a novel biomarker for gastric cancer ( 30 ). In one of our previous studies, it was shown that BANF1 was highly expressed in ESCC and that its expression was associated with the TNM stage and the tumor differentiation degree of ESCC patients ( 23 ).…”

<p>Expression and Prognostic Significance of BANF1 in Triple-Negative Breast Cancer</p>

“…11 In addition, Li reported that BANF1 expression is increased in gastric cancer and associated with the poor prognosis of patients, which was a novel biomarker for gastric cancer. 12 Our experimental results showed that the expression of BANF1 was significantly increased in TNBC tissues, indicating that BANF1 plays an important role in the carcinogenesis of TNBC. BANF1 protein was not only highly expressed in TNBC, but also related to the clinicalpathological parameter, such as the status of lymph node metastasis and TNM stage, which indicated that the progression of TNBC may be related to the expression of BANf1.…”

Absence of Nuclear p16 Is a Diagnostic and Independent Prognostic Biomarker in Squamous Cell Carcinoma of the Cervix

“…Importantly, we confirmed the colocalization of p16 with a protein subset previously involved in carcinogenesis with a specific role in nuclear shuttling. For instance, BANF1 has been reported to be involved in colony formation, migration and invasion in CC [37] and in the development of hepatocellular [23] and esophageal squamous cell [24] carcinomas and gastric cancer [26]. It is also known to interact with a protein which prevents nuclear accumulation of other proteins, such as β-catenin [38].…”

“…Some of the cytoplasmic p16 interactors identified by LC-MS/MS were selected for further examination by double immunofluorescence because of their involvement in other carcinogenic processes [23][24][25][26][27][28][29]. As seen in Figure 4C, we found that CDK4, the A-kinase anchor protein 8 (AKAP8), the barrier to autointegration factor (BANF1), and the type-1 angiotensin II receptor-associated protein (AGTRAP) colocalized with p16 in the cytoplasm of SiHa cells, confirming that these proteins interact with cytoplasmic p16 in SiHa cells.…”