Barrier protective effects of rutin in LPS-induced inflammation in vitro and in vivo

Lee, Wonhwa; Ku, Sae‐Kwang; Bae, Jong‐Sup

doi:10.1016/j.fct.2012.06.013

Cited by 80 publications

(58 citation statements)

References 46 publications

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“…(1) Herbal compounds could inhibit the binding and interactions of HMGB1 to its receptors. This concept was supported by the finding that herbal medicine blocks chemokine binding to its receptors [8][9][10][11][12][13][14]. (2) Herbal compounds could block the downstream signaling pathway of receptors activation by HMGB1; this is also supported by the finding that recently we showed that herbal compounds inhibited LPS-stimulated NF-κB activation which is the down-stream transcriptional factor of HMGB1 [8,11,[13][14][15][16][18][19][20][21][22].…”

supporting

confidence: 68%

“…This concept was supported by the finding that herbal medicine blocks chemokine binding to its receptors [8][9][10][11][12][13][14]. (2) Herbal compounds could block the downstream signaling pathway of receptors activation by HMGB1; this is also supported by the finding that recently we showed that herbal compounds inhibited LPS-stimulated NF-κB activation which is the down-stream transcriptional factor of HMGB1 [8,11,[13][14][15][16][18][19][20][21][22]. (3) Herbal compounds could enter cells and interact with proinflammatory intracellular kinases or signaling pathways such as NF-κB, c-Jun N-terminal kinases (JNK1/2) or signal transducer and activator of transcription 3 (STAT3) and inflammatory intracellular molecules such as interleukin-6 (IL-6), IL-8, monocyte chemoattractant protein 1 (MCP-1), TNF-α, IL-1β or atrogin-1.…”

mentioning

confidence: 54%

“…We recently showed that herbal single compound down-regulated the cell surface expression of the three receptors, TLR-2, TLR-4, and RAGE, which are known to bind HMGB1 to initiate pro-inflammatory responses in endothelial cells [8][9][10][11][12][13][14]. Similar to HMGB1, LPS also upregulated the expression of all three receptors on endothelial cells, and single compound from herbal medicines down regulated this effect [8][9][10][11][12][13][14][15][16][17][18], suggesting that herbal compounds can inhibit the amplification of pro-inflammatory pathways propagated by LPS and HMGB1 during infections. There are several potential mechanisms by which herbal compounds could interfere with HMGB1 functions.…”

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confidence: 99%

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Potential Herbal Therapy of HMGB1-Targeting Agent in Sepsis

Bae¹

2012

Biochem & Pharmacol

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confidence: 68%

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confidence: 54%

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confidence: 99%

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Potential Herbal Therapy of HMGB1-Targeting Agent in Sepsis

Bae¹

2012

Biochem & Pharmacol

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“…Rutin 37.25 mg/g, and quercetin 6.09 mg/g were detected and quantified in the HECO with HPLC. Rutin is known for having antiplatelet, antiviral, antihypertensive (Lee et al 2012;Li et al 2012), and antioxidant effects (Hubinger et al 2010), and is used for venous and lymphatic insufficiency, capillary fragility (Baby et al 2008), and also as a protector against solar damage (Velasco et al 2008). Quercetin is a flavonoid that occurs naturally in plants, with important effects on oxidative stress (Prince et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of Calendula officinalis L. leaves

Deuschle

Piana

et al. 2015

Rev. bras. plantas med.

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ABSTRACT:The plant Calendula officinalis L. is widely applied due to its medicinal properties, which are mainly dermatological and ornamental. The goal of this study is to assess the phytochemical components in a hydroethanolic extract (HECO) from the leaves of Calendula officinalis L. using UV-VIS spectrophotometry and thin layer chromatography (TLC), as well as to identify and quantify the components related to its antioxidant capacity employing high performance liquid chromatographic (HPLC). The antioxidant capacity evaluation was performed using the DPPH method for superoxide and hydroxyl radicals. The photo-protective capacity was evaluated by UVspectrophotometry in order to determine the in vitro Sun Protection Factor(SPF). The results show the plant's strong antioxidant activity (DPPH and hydroxyl methods), which we believe to be related to the presence of flavonoids (24.67 mg/g), polyphenols (33.90 mg/g), condensed tannins (27.30 mg/g), and the amount of rutin (37.25 mg/g), and quercetin (6.09 mg/g) found during the study. The HECO presented a good antioxidant capacity, most likely due to the polyphenols, flavonoids, and tannins in its contents. However, the obtained SPF of 1.89 ± 0.05 does not allow the plant to be classified as a stand-alone sunscreen, and more studies are needed in order to test its ability to enhance sunscreens in existing cosmetic formulations.Keywords: Calendula officinalis. Marigold. Antioxidant. Phenolic Compounds. Photo-protection. RESUMO:Avaliação fitoquímica e capacidade antioxidante e fotoprotetora in vitro das folhas de Calendula officinalis L. A Calendula officinalis L. é uma planta amplamente utilizada por suas propriedades medicinais, principalmente dermatológica e ornamental. O objetivo deste estudo é avaliar constituintes fitoquímicos do extrato hidroetanólico das folhas de Calêndula officinalis L. (HECO) por espectrofotometria UV-visível e cromatografia em camada delgada (CCD), bem como detectar e quantificar os componentes relacionados com a capacidade antioxidante por Cromatografia Líquida de Alta Eficiência (CLAE). Posteriormente, foi avaliada a capacidade antioxidante pelo método do DPPH, radicais superóxido e hidroxila. Além disso, a capacidade fotoprotetora foi avaliada através de espectrofotometria UV para determinação do Fator de Proteção Solar in vitro (FPS). Os resultados evidenciaram que a planta apresentou uma excelente atividade antioxidante para o método do DPPH e do radical hidroxila, o que pode estar relacionada com a presença de flavonoides (24,67 mg/g), polifenóis (33,90 mg/g) e taninos condensados (27,30 mg/g) e da quantidade de rutina (37,25 mg/g) e quercetina (6,09 mg/g) encontrados neste estudo. O HECO apresentou uma notável capacidade antioxidante, provavelmente devido à presença de polifenóis, flavonoides e taninos. O valor do FPS encontrado (1,89 ± 0,05), não classifica a planta como um protetor solar isolado. No entanto, mais estudos são necessários para testar a capacidade de potencializar filtros solares em formulações cosméticas.

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“…Cell adhesion molecules (CAMs), including selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), play critical roles in cell adhesion to the vascular endothelium resulting in extravasation of cells and vascular inflammation. Rutin can inhibit the expression of CAMs, thus inhibiting the adhesion and migration of leukocytes and affecting vascular barrier integrity [10,11]. Vitamin C, or ascorbic acid, can also be used in CCHF and epidemic hemorrhagic fever [11].…”

mentioning

confidence: 99%