Barley (Hordeum vulgare L.) Transformation Using Immature Embryos

Marthe, Cornelia; Kumlehn, Jochen; Hensel, Goetz

doi:10.1007/978-1-4939-1695-5_6

Cited by 30 publications

(32 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, cv. Golden Promise remains the cultivar routinely used for high-throughput barley transformation studies [39, 40]. Therefore it was hypothesized that the mutation causing the semi-dwarf phenotype of cv.…”

Section: Discussionmentioning

confidence: 99%

HvDep1 Is a Positive Regulator of Culm Elongation and Grain Size in Barley and Impacts Yield in an Environment-Dependent Manner

et al. 2016

View full text Add to dashboard Cite

Heterotrimeric G proteins are intracellular membrane-attached signal transducers involved in various cellular processes in both plants and animals. They consist of three subunits denoted as α, β and γ. The γ-subunits of the so-called AGG3 type, which comprise a transmembrane domain, are exclusively found in plants. In model species, these proteins have been shown to participate in the control of plant height, branching and seed size and could therefore impact the harvestable yield of various crop plants. Whether AGG3-type γ-subunits influence yield in temperate cereals like barley and wheat remains unknown. Using a transgenic complementation approach, we show here that the Scottish malting barley cultivar (cv.) Golden Promise carries a loss-of-function mutation in HvDep1, an AGG3-type subunit encoding gene that positively regulates culm elongation and seed size in barley. Somewhat intriguingly, agronomic field data collected over a 12-year period reveals that the HvDep1 loss-of-function mutation in cv. Golden Promise has the potential to confer either a significant increase or decrease in harvestable yield depending on the environment. Our results confirm the role of AGG3-type subunit-encoding genes in shaping plant architecture, but interestingly also indicate that the impact HvDep1 has on yield in barley is both genotypically and environmentally sensitive. This may explain why widespread exploitation of variation in AGG3-type subunit-encoding genes has not occurred in temperate cereals while in rice the DEP1 locus is widely exploited to improve harvestable yield.

show abstract

Section: Discussionmentioning

confidence: 99%

HvDep1 Is a Positive Regulator of Culm Elongation and Grain Size in Barley and Impacts Yield in an Environment-Dependent Manner

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Immature embryos of barley cv Golden Promise were transformed with the binary vectors using the Agrobacterium tumefaciens strain AGL1 as described (Hensel et al, 2008). Homozygous transgenic plants were obtained by double haploid technology (Marthe et al, 2015). The presence of the antibiotic resistance marker was confirmed by PCR using specific primer pairs (Hensel et al, 2008).…”

Section: Plant Materialsmentioning

confidence: 99%

HvPap-1 C1A Protease and HvCPI-2 Cystatin Contribute to Barley Grain Filling and Germination

et al. 2016

Self Cite

View full text Add to dashboard Cite

Proteolysis is an essential process throughout the mobilization of storage proteins in barley (Hordeum vulgare) grains during germination. It involves numerous types of enzymes, with C1A Cys proteases the most abundant key players. Manipulation of the proteolytic machinery is a potential way to enhance grain yield and quality, and it could influence the mobilization of storage compounds along germination. Transgenic barley plants silencing or over-expressing the cathepsin F-like HvPap-1 Cys protease show differential accumulation of storage molecules such as starch, proteins, and free amino acids in the grain. It is particularly striking that the HvPap-1 artificial microRNA lines phenotype show a drastic delay in the grain germination process. Alterations to the proteolytic activities in the over-expressing and knock-down grains associated with changes in the level of expression of several C1A peptidases were also detected. Similarly, down-regulating cystatin Icy-2, one of the proteinaceous inhibitors of the cathepsin F-like protease, also has important effects on grain filling. However, the ultimate physiological influence of manipulating a peptidase or an inhibitor cannot be always predicted, since the plant tries to compensate the modified proteolytic effects by modulating the expression of some other peptidases or their inhibitors.

show abstract

“…In these experiments, the efficiency of transformation of Golden Promise was increased to 86.7% and six of nine barley cultivars previously recalcitrant to transformation were transformable at low frequencies (0.2-7.8%). Further improvement of this protocol has enabled the transformation of more than 20 barley cultivars but, prior to the work reported here, the transformation efficiency of Golden Promise remained higher than that of any other cultivar tested in similar conditions (Marthe et al, 2015).…”

Section: Discussionmentioning

confidence: 79%

TRA1: A Locus Responsible for Controlling Agrobacterium-Mediated Transformability in Barley

Orman-Ligeza¹,

Harwood²,

Hedley³

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

In barley (Hordeum vulgare L.), Agrobacterium-mediated transformation efficiency is highly dependent on genotype with very few cultivars being amenable to transformation. Golden Promise is the cultivar most widely used for barley transformation and developing embryos are the most common donor tissue. We tested whether barley mutants with abnormally large embryos were more or less amenable to transformation and discovered that mutant M1460 had a transformation efficiency similar to that of Golden Promise. The large-embryo phenotype of M1460 is due to mutation at the LYS3 locus. There are three other barley lines with independent mutations at the same LYS3 locus, and one of these, Risø1508 has an identical missense mutation to that in M1460. However, none of the lys3 mutants except M1460 were transformable showing that the locus responsible for transformation efficiency, TRA1, was not LYS3 but another locus unique to M1460. To identify TRA1, we generated a segregating population by crossing M1460 to the cultivar Optic, which is recalcitrant to transformation. After four rounds of backcrossing to Optic, plants were genotyped and their progeny were tested for transformability. Some of the progeny lines were transformable at high efficiencies similar to those seen for the parent M1460 and some were not transformable, like Optic. A region on chromosome 2H inherited from M1460 is present in transformable lines only. We propose that one of the 225 genes in this region is TRA1.

show abstract

Barley (Hordeum vulgare L.) Transformation Using Immature Embryos

Cited by 30 publications

References 35 publications

HvDep1 Is a Positive Regulator of Culm Elongation and Grain Size in Barley and Impacts Yield in an Environment-Dependent Manner

HvDep1 Is a Positive Regulator of Culm Elongation and Grain Size in Barley and Impacts Yield in an Environment-Dependent Manner

HvPap-1 C1A Protease and HvCPI-2 Cystatin Contribute to Barley Grain Filling and Germination

TRA1: A Locus Responsible for Controlling Agrobacterium-Mediated Transformability in Barley

Contact Info

Product

Resources

About