HvPap-1 C1A Protease and HvCPI-2 Cystatin Contribute to Barley Grain Filling and Germination

Díaz-Mendoza, Mercedes; Domínguez-Figueroa, José; Velasco-Arroyo, Blanca; Cambra, Inés; González‐Melendi, Pablo; López‐Gonzálvez, Ángeles; García, Aitana Martos; Hensel, Goetz; Kumlehn, Jochen; Dı́az, Isabel; Martínez, Manuel

doi:10.1104/pp.15.01944

Cited by 34 publications

(53 citation statements)

References 47 publications

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“…These enzymes play a role in plant senescence, programmed cell death (PCD), and proteolysis mediated by abiotic stress, among other processes. 13,14 In animals, cathepsins are well-known lysosomal proteases with a role in autophagy and cell death, 15 in which they have a critical role in the degradation of proteins within autolysosomes, after autophagosome fusion. [16][17][18] However, the role of plant cathepsins in autophagy is still unresolved.…”

Section: Introductionmentioning

confidence: 99%

Modulation of autophagy and protease activities by small bioactive compounds to reduce cell death and improve stress-induced microspore embryogenesis initiation in rapeseed and barley

Pérez-Pérez¹,

Bárány²,

Berenguer³

et al. 2018

Plant Signaling & Behavior

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Microspore embryogenesis is a powerful biotechnological tool that is very useful in crop breeding for the rapid production of haploid and double-haploid embryos and plants. In this in vitro system, the haploid microspore is reprogrammed by the application of specific stress treatments. A high level of cell death after the stress is a major factor that greatly reduces embryogenesis yield at its initial stages. Autophagy is a degradation pathway that is present in all eukaryotes and plays key roles in a range of processes, including stress responses. Many proteases participate in autophagy and cell death; among them, cathepsins are the most abundant enzymes with a role in plant senescence and programmed cell death (PCD). Moreover, although plant genomes do not contain homologues of caspases, caspase 3-like activity (main executioner protease of animal cell death) has been detected in many plant PCD processes. Recent studies by our group in barley microspore cultures reported that the stress treatment required for inducing microspore embryogenesis (cold treatment), also produced reactive oxygen species (ROS) and cell death, concomitantly with the induction of autophagy, as well as cathepsin-like and caspase 3-like proteolytic activities. In the present study, we report new data on microspore embryogenesis of rapeseed that indicate, as in barley, activation of cell death and autophagy processes after the inductive stress. The results revealed that treatments modulating autophagy and proteases produced the same effect in the two plant systems, regardless of the stress applied, cold in barley or heat in rapeseed. Pharmacological treatments with small bioactive compounds that inhibit ROS, autophagy and specific cell death-proteases led to reduced cell death and an increased embryogenesis initiation rate in both, barley and rapeseed. Taken together, these findings open up new intervention pathways by modulating autophagy and proteases, which are very promising in terms of increasing the efficiency of in vitro microspore embryogenesis systems for biotechnological applications in crop breeding.

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Section: Introductionmentioning

confidence: 99%

Modulation of autophagy and protease activities by small bioactive compounds to reduce cell death and improve stress-induced microspore embryogenesis initiation in rapeseed and barley

Pérez-Pérez¹,

Bárány²,

Berenguer³

et al. 2018

Plant Signaling & Behavior

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“…Visualization of aligned datasets to inspect the quality of feature assignment is very practical. Such option together with the possibility for manual peak re-integration is available in MassHunterProfinder software (Agilent Technologies) [113].…”

Section: Discussionmentioning

confidence: 99%

Capillary Electrophoresis Mass Spectrometry as A Tool for Untargeted Metabolomics

et al. 2016

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Highly polar and ionic metabolites, such as sugars, most amino acids, organic acids or nucleotides are not retained by conventional reversed-phase LC columns and polar stationary phases and hydrophilic-interaction LC lacks of robustness, which is still limiting their applications for untargeted metabolomics where reproducibility is a must. Biological samples such as blood, urine or even tissues include many hydrophilic compounds secreted from cells, their analysis is essential for biomarker discovery, disease progression or treatment effects. This review focuses on CE coupled to MS as a mature technique for untargeted metabolomics including sample pretreatment, types of matrices, analytical methods, applications and data treatment strategies for polar compound analysis in biological matrices. The main applications and results of CE-MS in untargeted metabolomics are discussed and presented in a tabulated format.

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“…PLCPs in plants are divided Programmed Cell Death in Seeds: An Adaptive Mechanism Required for Life DOI: http://dx.doi.org /10.5772/intechopen.86833 into nine subfamilies. Thus, 32, 41, and 45 PLCPs' members have been identified in Arabidopsis, barley, and rice, respectively [49]. PLCPs have no structural relationship to the caspases, and its natural competitive and reversible inhibitors are the phytocystatins which are evolutionarily well conserved [50].…”

Section: Involvement Of Papain-like Kdel-cys-eps In Seed Lifementioning

confidence: 99%

“…During barley seed germination, PLCPs were secreted from the scutellar and the aleurone layers to the endosperm to degrade the endosperm Sps [58]. Recently, the results of overexpression and silencing of HvPap-1, a gibberellin (GAs)-induced PLCP gen, indicated that PLCPs are important factors in mobilizing SPs to promote seed germination, and their expression and/or activity are regulated by GAs, ABA, and cystatins [49]. Ricinosomes and nuclear DNA are fragmented during PCD.…”

Section: Involvement Of Papain-like Kdel-cys-eps In Seed Lifementioning

confidence: 99%

Programmed Cell Death in Seeds: An Adaptive Mechanism Required for Life

Matilla¹

2020

Seed Dormancy and Germination

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The regeneration of the mother plant through germinative process is the main reason that evolutionarily justifies the existence of a viable seed. Current knowledge indicates that the control of germination is a sophisticated process mainly controlled by hormones and reactive oxygen species (ROS), among other endogenous factors. One of the events that directly participate in the germination is the degradation of storage proteins (SPs). Thus, vacuolar processing enzymes (VPEs) contribute to SPs' degradation and mobilization due to direct proteolysis or through the activation of other peptidases. In parallel, the relationship between VPEs and programmed cell death (PCD) is beyond doubt. As an alternative to VPEs, the formation of vesicles called ricinosomes containing papain-like Cys-proteases (PLCPs) and located in the reserve tissues of some germinating seeds also collaborates to protein degradation. Finally, there are increasing evidences linking nucleases to PCD in different tissues of seed. However, its state of the art is still little developed. Together, this current overview illustrates a part of the complexity of PCD in seeds, a puzzle far from being solved.

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HvPap-1 C1A Protease and HvCPI-2 Cystatin Contribute to Barley Grain Filling and Germination

Cited by 34 publications

References 47 publications

Modulation of autophagy and protease activities by small bioactive compounds to reduce cell death and improve stress-induced microspore embryogenesis initiation in rapeseed and barley

Modulation of autophagy and protease activities by small bioactive compounds to reduce cell death and improve stress-induced microspore embryogenesis initiation in rapeseed and barley

Capillary Electrophoresis Mass Spectrometry as A Tool for Untargeted Metabolomics

Programmed Cell Death in Seeds: An Adaptive Mechanism Required for Life

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