Barcoding helps threatened species: the case of <i>Iris marsica</i> (Iridaceae) from the protected areas of the Abruzzo (Central Italy)

Castro, Olga De; Guacchio, Emanuele Del; Iorio, Emanuela Di; Maio, Antonietta Di; Martino, Luciano Di; Bartolucci, Fabrizio; Conti, Fabio

doi:10.1080/11263504.2020.1762786

Cited by 10 publications

(6 citation statements)

References 79 publications

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“…Cypripedium calceolus is included in the LIFE FLORANET project "LIFE15 NAT/IT/000946," the main objective of which is to protect and to improve the conservation status of seven vascular plants listed under the Habitats Directive 92/43/EEC, occurring in Natura 2000 areas in the Central Apennines. During the project, started in 2016 and still in progress, ecological, floristic and taxonomic aspects have been also investigated De Castro et al, 2020;. Several concrete actions have been carried out for in situ and ex situ conservation and management of C. calceolus.…”

Section: Introductionmentioning

confidence: 99%

Combining current knowledge of Cypripedium calceolus with a new analysis of genetic variation in Italian populations to provide guidelines for conservation actions

Gargiulo

Adamo

Cribb

et al. 2021

Conservat Sci and Prac

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The split between conservation science and real-world application is an ongoing issue despite several calls for unification. Researchers are empowered to partially bridge the research-implementation gap by making their findings more accessible. Cypripedium calceolus is the most recognizable orchid of the European flora, and is currently facing habitat change and fragmentation, in addition to threats from collectors and illegal traders. Although several studies have focused on the ecological and genetic features of the species, a comprehensive account of how such aspects can be translated into concrete conservation recommendations is still missing. In this study, we describe microsatellite genetic variation in 188 individuals from different Italian populations of C. calceolus. Our results indicate the need for immediate conservation action for the most isolated populations in the Central Apennines and north-western Italy. Although our genetic findings are specific to the Italian populations, our aim is to review ecological and population genetic aspects in C. calceolus and

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Section: Introductionmentioning

confidence: 99%

Combining current knowledge of Cypripedium calceolus with a new analysis of genetic variation in Italian populations to provide guidelines for conservation actions

Gargiulo

Adamo

Cribb

et al. 2021

Conservat Sci and Prac

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“…De Castro et al 2020 and references therein;Koblmüller 2023). In this study, DNA barcoding was applied to test the identity of plants living near the Marmore waterfalls in central Italy, considered a new species, G. lucarinii, morphologically very similar to G. macrorrhizum(Wagensommer and Venanzoni 2021).…”

mentioning

confidence: 99%

WITHDRAWN: Molecular and cytogenetic analyses in Geranium macrorrhizum L. wild Italian plants

Cardinali,

Ceccarelli

2023

Preprint

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Geranium macrorrhizum L. is a herbaceous species native to southern Europe, and introduced in central Europe and North America. It is widely distributed also in Italy, up to Campania region as the southern limit. In this study, molecular and cytogenetic analyses were carried out on 22 wild plants, collected in central and southern Italy, compared to five cultivated plants, with the main purpose to identify those living near the Marmore waterfalls in central Italy, recently described as the new species G. lucarinii. Four barcoding markers (rbcL, matK, trnH-psbA intergenic spacer, and internal transcribed spacer region, ITS) were sequenced and their variability among the plants was evaluated. Chromosome numbers were determined and 45S rDNA was physically mapped by fluorescence in situ hybridization (FISH) in both wild and cultivated plants. Moreover, genomic affinity between wild and cultivated plants was evaluated by genomic in situ hybridization (GISH). Our results confirmed that all the plants, including Marmore population, belong to G. macrorrhizum. Barcoding analyses showed a close similarity among the wild plants, as well as a differentiation, although not significant, between the wild plants on one hand and the cultivated plants on the other. Integrated studies focusing on morphological, genetic and ecological characterization of a larger number of wild populations will allow for knowing the extent of the variability within the species.

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“…nuclear internal transcribed spacer region (ITS)) [ 8 ]. DNA barcoding has been largely used in basic and applied biodiversity research to discriminate between morphologically similar taxa, thus reducing the number of misidentifications, for cultivated flora analyses or to solve the doubtful status of some alien species ([ 9 ] and references therein, [ 10 ]).…”

Section: Introductionmentioning

confidence: 99%

Molecular and cytogenetic analyses in Geranium macrorrhizum L. wild Italian plants

Cardinali,

Ceccarelli

2024

R. Soc. Open Sci.

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Geranium macrorrhizum L. is a herbaceous species native to southern Europe and was introduced in central Europe and North America. It is also widely distributed in Italy. In this study, molecular and cytogenetic analyses were carried out on 22 wild plants, collected in central and southern Italy, compared with five cultivated plants, with the main purpose to identify those living near the Marmore waterfalls in central Italy, recently described as the new species Geranium lucarinii . Four barcoding markers ( rbcL , matK , trnH-psbA intergenic spacer and internal transcribed spacer region) were sequenced and their variability among the plants was evaluated. Chromosome numbers were determined and 45S rDNA was physically mapped by fluorescence in situ hybridization. Moreover, genomic affinity between wild and cultivated plants was evaluated by genomic in situ hybridization. The results of this study supported that all the plants belong to G. macrorrhizum , including the Marmore population. Barcoding analyses showed a close similarity among the wild plants, and a differentiation, although not significant, between the wild plants on one hand and the cultivated plants on the other. Integrated studies focusing on morphological, genetic and ecological characterization of a larger number of wild populations would allow us to know the extent of the variability within the species.

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Barcoding helps threatened species: the case of Iris marsica (Iridaceae) from the protected areas of the Abruzzo (Central Italy)

Cited by 10 publications

References 79 publications

Combining current knowledge of Cypripedium calceolus with a new analysis of genetic variation in Italian populations to provide guidelines for conservation actions

Combining current knowledge of Cypripedium calceolus with a new analysis of genetic variation in Italian populations to provide guidelines for conservation actions

WITHDRAWN: Molecular and cytogenetic analyses in Geranium macrorrhizum L. wild Italian plants

Molecular and cytogenetic analyses in Geranium macrorrhizum L. wild Italian plants

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