Barbaloin protects against lipopolysaccharide (LPS)-induced acute lung injury by inhibiting the ROS-mediated PI3K/AKT/NF-κB pathway

Jiang, Kangfeng; Guo, Shanchun; Yang, Chao; Yang, Jing; Chen, Yu; Shaukat, Aftab; Zhao, Gan; Wu, Haichong; Deng, Ganzhen

doi:10.1016/j.intimp.2018.08.023

Cited by 106 publications

(85 citation statements)

References 53 publications

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“…Studies reported that activation of the PI3K/AKT pathway induced NF‐κB mobilization (Jiang et al, ; Li et al, ). The experiment showed that AKT and NF‐κB significantly phosphorylated in LPS‐mediated BEND cells.…”

Section: Resultsmentioning

confidence: 99%

“…Studies reported that activation of the PI3K/AKT pathway induced NF-κB mobilization (Jiang et al, 2018;Li et al, 2015).…”

Section: Mir-488 Restrains Lps-mediated Activation Of the Akt/nf-κbmentioning

confidence: 99%

“…It was reported that LPS‐activated signaling pathways and Toll‐like receptors, protein kinase B κ‐light chain enhancement of the nuclear factor‐activated B cells (AKT/NF‐κB), the signaling molecules and downstream cytokines are synthesized and released once the signaling pathway is activated (Jiang et al, ). AKT/NF‐κB is an important signal transduction pathway in cells, which is involved in regulating various biological functions such as cell proliferation, cell differentiation, and apoptosis (Guo et al, ).…”

Section: Introductionmentioning

confidence: 99%

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miR‐488 mediates negative regulation of the AKT/NF‐κB pathway by targeting Rac1 in LPS‐induced inflammation

Liu

Guo

Jiang

et al. 2019

Journal Cellular Physiology

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Endometritis is an inflammatory change in the structure of the endometrium due to various causes and is a common cause of infertility. Studies have confirmed that microRNAs (miRNAs) play a key regulatory role in various inflammatory diseases.However, the miRNA-mediated mechanism of endometrial inflammation induced by lipopolysaccharides (LPS) remains unclear. In this study, real-time quantitative polymerase chain reaction, Western blot analysis, immunofluorescence and Rac family small GTPase 1 (Rac1) interference were used to reveal the overexpression of miR-488 in the LPS-induced bovine uterus, and the effect of protein kinase B κ-light chain enhancement of the nuclear factor-activated B cells (AKT/NF-κB) pathway in intimal epithelial cells. The results showed that the expression of inflammatory cytokines such as interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α in the experimental group was significantly lower than that in the control group when miR-488 was overexpressed. Similar results were observed in the expression levels of p-AKT, p-IKK, and p-p65 proteins. In addition, the dual-luciferase reporter system confirmed that miRNA-488 may directly target the 3′-untranslated region of Rac1. In turn, the expression of Rac1 was inhibited. Moreover, the nuclear translocation of NF-κB was inhibited, and meanwhile, the accumulation of reactive oxygen species (ROS) in the cells was reduced. Thus, we provide basic data for the negative regulation of miR-488 in LPS-induced inflammation by inhibiting ROS production and the AKT/NF-kB pathway in intimal epithelial cells. K E Y W O R D SAKT/NF-κB, endometritis, lipopolysaccharide, miR-488, ROS

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Section: Resultsmentioning

confidence: 99%

“…Studies reported that activation of the PI3K/AKT pathway induced NF-κB mobilization (Jiang et al, 2018;Li et al, 2015).…”

Section: Mir-488 Restrains Lps-mediated Activation Of the Akt/nf-κbmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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miR‐488 mediates negative regulation of the AKT/NF‐κB pathway by targeting Rac1 in LPS‐induced inflammation

Liu

Guo

Jiang

et al. 2019

Journal Cellular Physiology

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“…The change of gut microbiota may lead to barrier failure, which is a key event contributing to the severity of gut injury [13,14]. Toll-like receptor 4-(TLR4-) dependent phosphatidylinositol 3-kinase (PI3K)/Protein kinase B (AKT)/NF-κB signaling is closely associated with oxidative stress and inflammatory responses [15,16]. However, whether picroside II exerts its function on SAP-induced intestinal barrier injury or affects TLR4-dependent PI3K/AKT/NF-κB signaling remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Picroside II Improves Severe Acute Pancreatitis-Induced Intestinal Barrier Injury by Inactivating Oxidative and Inflammatory TLR4-Dependent PI3K/AKT/NF-κB Signaling and Improving Gut Microbiota

Piao¹,

Liu

Sui

et al. 2020

Oxidative Medicine and Cellular Longevity

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Background. Picroside II exerts anti-inflammatory and antidiarrheal effects for treating the diseases associated with oxidative injury. However, its function on pancreatitis-induced intestinal barrier injury remains unclear. Hypothesis/Purpose. We hypothesized that picroside II will have protective effects against pancreatitis-induced intestinal barrier injury by affecting oxidative and inflammatory signaling (Toll-like receptor 4- (TLR4-) dependent phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), and nuclear factor kappa B (NF-κB)). Study Design and Methods. A Sprague-Dawley (SD) rat model with severe acute pancreatitis (SAP) was induced via the injection of sodium taurocholate (4% wt/vol; 1 mL/kg). All rats were divided into 3 groups: sham (CG), SAP-induced intestinal barrier injury (MG), and picroside II (PG) groups. Intestinal barrier injury was assessed by scanning electron microscopy (SEM), hematoxylin and eosin staining, and pathological scores. We measured the levels of pancreatitis biomarkers (amylase and lipase), oxidative and inflammatory signaling (TLR4-dependent PI3K/AKT/NF-κB), oxidative stress marker (superoxidase dismutase (SOD), catalase (CAT), glutathione peroxidases (GPx), and malondialdehyde), and inflammatory markers (tumor necrosis factor α (TNFα), interleukin- (IL-) 1, IL-6, and IL-10) in serum and/or gut tissues. Gut microbiota composition in feces was measured by using 16S rRNA sequencing. Results. SEM showed that intestinal barrier injury was caused with the loss of intestinal villi and mitochondria destruction, and pathological scores were increased in the MG group. The levels of amylase, lipase, malondialdehyde, TNFα, IL-1, IL-6, TLR4, PI3K, AKT, and NF-κB were increased, and the levels of SOD, GPx, CAT, and IL-10 was reduced in the MG group when compared with CG group (P<0.05). Picroside II treatment inhibited the symptoms in the MG group and showed antioxidant and anti-inflammatory activities. The serum levels of picroside II had strong correlation with the levels of inflammatory and oxidative stress biomarkers (P<0.05). Picroside II treatment increased the proportion of Lactobacillus and Prevotella and decreased the proportion of Helicobacter and Escherichia_Shigella in the model. Conclusions. Picroside II improved the SAP-induced intestinal barrier injury in the rat model by inactivating oxidant and inflammatory signaling and improving gut microbiota.

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“…Most of the extracts used for inhibiting inflammation in traditional Chinese medications have “clearing and detoxifying” effects. Aloe vera extract can downregulate TNF‐α, IL‐1β and IL‐6 expression in macrophages in a mouse model of lipopolysaccharide (LPS)‐induced acute lung injury (ALI), thus potentially protecting LPS‐induced ALI . Other extracts used in traditional Chinese medicine, such as curcumin, emodin, berberine and grass coumarone, have similar effects.…”

Section: Exogenous Inducers Of Macrophage Heterogeneitymentioning

confidence: 99%

Heterogeneous macrophages: Supersensors of exogenous inducing factors

et al. 2019

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This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractAs heterogeneous immune cells, macrophages mount effective responses to various internal and external changes during disease progression. Macrophage polarization, rather than macrophage heterogenization, is often used to describe the functional differences between macrophages. While macrophage polarization partially contrib-

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Barbaloin protects against lipopolysaccharide (LPS)-induced acute lung injury by inhibiting the ROS-mediated PI3K/AKT/NF-κB pathway

Cited by 106 publications

References 53 publications

miR‐488 mediates negative regulation of the AKT/NF‐κB pathway by targeting Rac1 in LPS‐induced inflammation

miR‐488 mediates negative regulation of the AKT/NF‐κB pathway by targeting Rac1 in LPS‐induced inflammation

Picroside II Improves Severe Acute Pancreatitis-Induced Intestinal Barrier Injury by Inactivating Oxidative and Inflammatory TLR4-Dependent PI3K/AKT/NF-κB Signaling and Improving Gut Microbiota

Heterogeneous macrophages: Supersensors of exogenous inducing factors

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