Band broadening in size-exclusion chromatography of polydisperse samples

Popovici, Simona-Tereza; Kok, Wim Th.; Schoenmakers, Peter J.

doi:10.1016/j.chroma.2004.05.099

Cited by 67 publications

(54 citation statements)

References 35 publications

(71 reference statements)

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“…4b) is largely due to the difference in column length and flow rate. From flow rates, we have found [21,33] that H is proportional to u 0 0.21 , when u 0 is the linear (interparticle) velocity. Therefore, we can expect…”

Section: Resultsmentioning

confidence: 96%

“…In the studied systems (using a single 250-mm column in the first dimension), the observed bandwidths for narrow PS standards were clearly dominated by chromatographic dispersion. This indicates that assumptions made elsewhere [21,33] for constructing SEC integrity plots were justified.…”

Section: Discussionmentioning

confidence: 94%

“…Experimental results from previous work [21] indicate that in SEC of narrow polymer standards band broadening is dominated by chromatographic dispersion. This implies that…”

Section: Theorymentioning

confidence: 93%

“…In [21], II SEC was plotted (for specific columns under specific conditions) as a function of the sample PDI and M r . From such figures (called integrity plots), quantitative information can be obtained on the performance of SEC systems for samples with different M r and PDI values.…”

Section: Theorymentioning

confidence: 99%

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Two‐dimensional chromatography as a tool for studying band broadening in size‐exclusion chromatography

Popovici

Horst

Schoenmakers

2005

J of Separation Science

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Two-dimensional chromatography as a tool for studying band broadening in size-exclusion chromatographyComprehensive 2-D size-exclusion chromatography (SEC6SEC) has been realized. SEC6SEC is not a useful technique for characterizing complex polymers. However, it is potentially an elegant tool to study band-broadening phenomena. If narrow fractions can be collected from the first dimension, the band broadening in the second dimension is only due to chromatographic dispersion. This would allow a clear distinction to be made between chromatographic band broadening (column and extracolumn) and SEC selectivity (band broadening due to sample polydispersity). In comparison with MALDI-MS, SEC6SEC allows the study of polymers across a much broader molar-mass range.

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Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 94%

“…Experimental results from previous work [21] indicate that in SEC of narrow polymer standards band broadening is dominated by chromatographic dispersion. This implies that…”

Section: Theorymentioning

confidence: 93%

Section: Theorymentioning

confidence: 99%

See 2 more Smart Citations

Two‐dimensional chromatography as a tool for studying band broadening in size‐exclusion chromatography

Popovici

Horst

Schoenmakers

2005

J of Separation Science

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“…[49,[52][53][54][55]), but they have not yet been implemented in commercial SEC data manipulation software. For a full description of the chromatographic and sample-related aspects that affect band broadening and their effect on obtaining reliable size distributions of polydisperse polymers, the review from Popovici et al is recommended [56].…”

Section: Band Broadeningmentioning

confidence: 99%

Characterization of branched polysaccharides using multiple‐detection size separation techniques

Vilaplana

Gilbert

2010

J of Separation Science

234

151

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The structure of branched polysaccharides involves a hierarchy of levels, from the constituent sugars, then the branching pattern, up to the macromolecular architecture, and then supramolecular organization. Finding causal relations between this complex structure/architecture and both (bio)synthetic mechanisms and final properties is needed for understanding the functionality of branched polysaccharides, which is important in fields ranging from improved nutrition and health through to papermaking and pharmaceuticals. The structural complexity makes this task especially challenging. This review focuses on the best current means to obtain reliable branch chain and size distributions using size-separation technologies coupled with number-, mass- and molecular-weight-sensitive detectors. Problems with current technologies are also critically appraised.

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Size Exclusion Chromatography

Podzimek

2022

Encyclopedia of Polymer Science and Technology

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Historical development of size exclusion chromatography is briefly outlined. The method is introduced as the only widely applicable analytical technique for the determination of molar mass distribution of synthetic and natural polymers. Different procedures of the molar mass determination including column calibration, universal calibration, and light scattering detection are provided. Various separation mechanisms, resolution, efficiency, selectivity, and secondary mechanisms are described. The effect of key operational parameters on the correctness of molar mass is discussed. In addition to the molar mass, the focus is on the characterization of long‐chain branching. Various applications of online viscometer are reviewed.

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Band broadening in size-exclusion chromatography of polydisperse samples

Cited by 67 publications

References 35 publications

Two‐dimensional chromatography as a tool for studying band broadening in size‐exclusion chromatography

Two‐dimensional chromatography as a tool for studying band broadening in size‐exclusion chromatography

Characterization of branched polysaccharides using multiple‐detection size separation techniques

Size Exclusion Chromatography

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