Characterization of branched polysaccharides using multiple‐detection size separation techniques

Vilaplana, Francisco; Gilbert, Robert G.

doi:10.1002/jssc.201000525

Cited by 234 publications

(155 citation statements)

References 109 publications

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“…(Vilaplana & Gilbert, 2010). This was implemented with pullulan standards (Polymer Standards Services, Mainz, Germany) with a mass range of 342 -2.35×10 6 .…”

Section: Analytical Secmentioning

confidence: 99%

A new non-degradative method to purify glycogen

Tan

Sullivan

Gao

et al. 2016

Carbohydrate Polymers

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A new non-degradative method to purify glycogen.Carbohydrate Polymers http://dx.doi.org/10. 1016/j.carbpol.2016.04.009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…(Vilaplana & Gilbert, 2010). This was implemented with pullulan standards (Polymer Standards Services, Mainz, Germany) with a mass range of 342 -2.35×10 6 .…”

Section: Analytical Secmentioning

confidence: 99%

A new non-degradative method to purify glycogen

Tan

Sullivan

Gao

et al. 2016

Carbohydrate Polymers

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“…The size dependences of the (log ), can be obtained from combined MALLS and concentration detectors (DRI). Light scattering provides absolute weightaverage MW size in solution and the intensity of the light scatted by the macromolecule is dependent on both concentration and MW (Vilaplana & Gilbert, 2010 ( ) and ( , ) , the (log ) of the samples at each hydrodynamic volume were calculated using the following equation:…”

Section: Size Exclusion Chromatography (Sec)mentioning

confidence: 99%

Microstructure and mechanical properties of arabinoxylan and (1,3;1,4)-β-glucan gels produced by cryo-gelation

López-Sánchez

Wang

Zhang

et al. 2016

Carbohydrate Polymers

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Microstructure and mechanical properties of arabinoxylan and (1,3;1,4)-beta;-glucan gels produced by cryogelation.Carbohydrate Polymers http://dx.doi.org/10. 1016/j.carbpol.2016.06.038 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractThe interactions between heteroxylans and mixed linkage glucans determine the architecture and mechanical properties of cereal endosperm cell walls. In this work hydrogels made of cross-linked arabinoxylan with addition of β-glucan were synthesised by cryogelation as a biomimetic tool to investigate endosperm walls. Molecular and microstructural properties were characterised by nuclear magnetic resonance ( 13 C NMR), scanning electron microscopy (SEM) and immunolabelling/confocal laser scanning 2 microscopy (CLSM). The response to mechanical stress was studied by compressionrelaxation experiments. The hydrogels consisted of a scaffold characterised by dense walls interconnected by macropores with both hemicelluloses co-localised and homogeneously distributed. The gels showed a high degree of elasticity reflected in their ability to resist compression without developing cracks and recover 60-80% of their original height. Our results highlight the compatibility of these hemicelluloses to coexist in confined environments such as cell walls and their potential role in determining mechanical properties in the absence of cellulose.

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“…NMR spectroscopy can be used to check for any remnant a-(1-6) linkages. [7,8] Three methods are commonly used to obtain N de (X) from the glucan chains: fluorophore-assisted carbohydrate electrophoresis (FACE), [9,10] high performance anion exchange chromatography (HPAEC), [11][12][13] and size-exclusion chromatography [14] (SEC, also sometimes termed GPC, gel-permeation chromatography). Fig.…”

Section: Techniques and Assumptionsmentioning

confidence: 99%

“…The well known method for going from the light-scattering signals at different angles and M W and R g,z is given, for example, in a recent review. [14] It is noted that an accurate determination of dn/dc is essential in this regard; this is best done using differential refractometry, for which commercial instruments are available.…”

Section: Techniques and Assumptionsmentioning

confidence: 99%

“…While there is no a priori reason for the two measures of size, R g and R h , to be related (the former is the square root of the mass average of the square of position, while R h is found through V h in Eqn 4, and is related to viscosity), it is seen that the two are close over the size range for which data are available, which is essentially that of amylopectin. [14] Some structure-property relations from Level 2 data are now given. Processing of starch-related materials by both milling (as in flour-making) and extrusion (as in making pasta and, in combination with appropriate additional substances, in making biomaterials) involves significant changes in Level 2 structural features.…”

Section: Some Structure-property Relationsmentioning

confidence: 99%

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Characterization Methods for Starch-Based Materials: State of the Art and Perspectives

Witt

Gilbert

2013

Aust. J. Chem.

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Improving starch-containing materials, whether food, animal feed, high-tech biomaterials, or engineering plastics, is best done by understanding how biosynthetic processes and any subsequent processing control starch structure, and how this structure controls functional properties. Starch structural characterization is central to this. This review examines how information on the three basic levels of the complex multi-scale structure of starch -individual chains, the branching structure of isolated molecules, and the way these molecules form various crystalline and amorphous arrangements -can be obtained from experiment. The techniques include fluorophore-assisted carbohydrate electrophoresis, multipledetector size-exclusion chromatography, and various scattering techniques (light, X-ray, and neutron). Some examples are also given to show how these data provide mechanistic insight into how biosynthetic processes control the structure and how the various structural levels control functional properties.

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Characterization of branched polysaccharides using multiple‐detection size separation techniques

Cited by 234 publications

References 109 publications

A new non-degradative method to purify glycogen

A new non-degradative method to purify glycogen

Microstructure and mechanical properties of arabinoxylan and (1,3;1,4)-β-glucan gels produced by cryo-gelation

Characterization Methods for Starch-Based Materials: State of the Art and Perspectives

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