Bancroftian Filariasis and Membrane Filters: Are Night Surveys Necessary? *

Dt, Dennis; McConnell, E.; Gb, White

doi:10.4269/ajtmh.1976.25.257

Cited by 21 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sera were stored in liquid nitrogen or at -70'C until the time of assay. Microfilaremia was assessed by membrane (Nucleopore Corp., Pleasanton, CA) filtration of 1 ml of heparinized cord and maternal blood (11 Filaria-specific IgE was measured by direct solid-phase RIA using adult B. malayi antigens as previously described (13,14). Briefly, sera were preabsorbed with 1% Sepharose 4B (Pharmacia Fine Chemicals) overnight, then incubated in plastic tubes with B. malayi antigens covalently bound to CNBr-activated Sepharose 4B in radioallergosorbent test (RAST) buffer (phosphate-buffered saline, pH.…”

Section: Methodsmentioning

confidence: 99%

Prenatal allergic sensitization to helminth antigens in offspring of parasite-infected mothers.

Weil¹,

Hussain²,

Kumaraswami³

et al. 1983

J. Clin. Invest.

119

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Prenatal allergic sensitization to helminth antigens in offspring of parasite-infected mothers.

Weil¹,

Hussain²,

Kumaraswami³

et al. 1983

J. Clin. Invest.

119

View full text Add to dashboard Cite

“…Microfilaremia was determined by filtration of 1 ml heparinized venous blood obtained between 10 p.m. and 2 a.m. through Nuclepore membranes of 5um pore size (Pleasanton, CA, USA) as in Dennis et al (1976). Membrane filters were Giemsa stained and microscopically examined for Mf.…”

Section: Microfilaria Detectionmentioning

confidence: 99%

Age and gender specific patterns of microfilaremia and circulating antigen with specific lymphocyte proliferative responses in Wucherereia bancrofti filariasis.

Al-Shafei¹,

El-Nashar²,

Ghoneame³

et al. 2002

The Egyptian Journal of Hospital Medicine

View full text Add to dashboard Cite

A population from a W. bancrofti-endemic area was evaluated by use of microfilaria(Mf) and adult worm circulating antigen(CAg) enzyme linked immunosorbent assay to demonstrate the relation of these two parameters to age , gender and the clinical status (symptomatic or asymptomatic) of the examined individuals. In addition, antigen-driven T cell proliferation was measured to study the effect of the clinical status and / or infection status on the host responsiveness in lymphatic filariasis. The results showed that the prevalence rates of each of the two markers are clearly age dependent and were significantly higher in males than females. The antigen prevalence rate was consistently higher than the microfilaria prevalence rate in all age groups. Among the asymptomatic and symptomatic patients, the direct relationship between Mf+ and CAg+ remained intact regardless of clinical status. Individuals positive for CAg had significantly lower lymphocyte proliferation responses to parasite antigen than did CAg-negative people. This study re-emphasizes the importance of CAg measurements for accurately assessing the clinical status of filariasis and demonstrates that the presence or absence of active infection, not clinical status is most closely associated with the immune responsiveness in lymphatic filariasis.

show abstract

“…Plasma specimens for the present study were selected from the Kafr Tahoria collection to represent various groups defined on the basis of infection status and age as noted below. Microfilaremia was detected by 5 mM membrane filtration of 1 ml blood samples collected at night (Dennis et al 1976). W. bancrofti antigens were detected in plasma by direct-sandwich ELISA as previously described (Ramzy et al 1994).…”

Section: Clinical Specimens and Classification Of Human Subjectsmentioning

confidence: 99%

Human antibody responses to Wuchereria bancrofti infective larvae

Helmy

Weil

Faris

et al. 2000

Parasite Immunology

View full text Add to dashboard Cite

Human IgG antibody responses to Wuchereria bancrofti third stage infective larvae (L3) surface and somatic antigens were studied by indirect immunofluorescence (IFA) and immunoblot with endemic Egyptian sera (n = 115) with the aim of identifying targets of protective immunity. Human sera variably recognized 14 major bands in L3 by immunoblot. The statistical significance of group differences in antibody prevalence was assessed by the chi-squared test. Children and young adults (aged 10-20 years) tended to have antibodies to more L3 somatic antigens than older adults, with significant differences for bands at 66, 60 and 5 kDa. Infected subjects had more consistent antibody responses to antigens at 55, 50 and 6 kDa than endemic normal subjects with negative serum filarial antigen tests, who are presumed to be uninfected. A 5 kDa antigen was preferentially recognized by the latter group. Antibodies to L3 surface antigens were equally prevalent in uninfected children (75%) and adults (90%) but less prevalent in people with microfilaremia (38%) than in amicrofilaremic subjects with or without filarial antigenemia (81%) (P < 0.001). IFA-positive sera showed significantly enhanced recognition of antigens at 66, 40 and 14 kDa in immunoblots relative to IFA-negative sera. Additional studies are needed to further characterize antigens identified in this study and to establish whether they are indeed targets of protective immunity in humans.

show abstract

Bancroftian Filariasis and Membrane Filters: Are Night Surveys Necessary? *

Cited by 21 publications

References 0 publications

Prenatal allergic sensitization to helminth antigens in offspring of parasite-infected mothers.

Prenatal allergic sensitization to helminth antigens in offspring of parasite-infected mothers.

Age and gender specific patterns of microfilaremia and circulating antigen with specific lymphocyte proliferative responses in Wucherereia bancrofti filariasis.

Human antibody responses to Wuchereria bancrofti infective larvae

Contact Info

Product

Resources

About