Banana MaWRKY49 and MaWRKY111 cooperate with MabZIP21 to activate the transcription of MaACS1 and MaACO1 during fruit ripening

Wu, Caixia; Su, Xiaolu; Shan, Wei; Chen, Yana; Yang, Yingying; Wei, Wei; Chen, Jianye; Lu, Wang-jin; Kuang, Jian-fei

doi:10.1016/j.postharvbio.2022.112087

Cited by 9 publications

(3 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 ), suggesting the likelihood that MabHLH28 may directly or indirectly regulate ethylene biosynthesis by interacting with other TFs that target ethylene biosynthetic genes. Previously, MaWRKY49 and MaWRKY111 were reported to function in banana fruit ripening by targeting ethylene formation genes MaACS1 and MaACO1 [ 25 ]. To detect whether MabHLH28 interacts with MaWRKY49/111, we firstly performed yeast two-hybrid (Y2H) assays.…”

Section: Resultsmentioning

confidence: 99%

“…Taken together, the mechanism by which the interaction between MabHLH28 and MaWRKY49/111 synergistically activates the transcription of softening-associated genes could be explained by the above two reasons. In addition, as previous work has revealed that MaWRKY49/111 physically interact with MabZIP21 [ 25 ], further investigations were needed to study the biochemical and genetic machinery of how MabHLH28, MaWRKY49/111, and MabZIP21 proteins act together to mediate banana fruit ripening and softening. Moreover, given the previous findings that MaMYB4 interacts with MaBRG2/3 and MaMAPK14 to undergo protein modifications [ 62 , 63 ], functional connectivity between MabHLH28 and the proteins involved in protein modifications presents opportunities for stripping away MabHLH28 under post-translational regulation during fruit ripening in a future direction.…”

Section: Discussionmentioning

confidence: 99%

“…In apple fruit, MdWRKY126 regulates malate accumulation by activating the expression of cytosolic malate dehydrogenase 5 ( MdMDH5 ) [ 24 ]. In banana fruit, MaWRKY49/111 bind to and activate the 1-amino-cyclopropane-1-carboxylic acid oxidase 1 ( MaACO1 ) and MaACS1 promoters [ 25 ], although studies have shown that WRKY TFs play an important role in fruit ripening and softening. However, the current understanding of WRKY-interacting ripening-related TFs is limited, and their specific regulatory mechanisms are unknown.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Banana MabHLH28 positively regulates the expression of softening-related genes to mediate fruit ripening independently or via cooperating with MaWRKY49/111

Wu,

Cai,

et al. 2024

Horticulture Research

Self Cite

View full text Add to dashboard Cite

Texture softening is a physiological indicator of fruit ripening, which eventually contributes to fruit quality and consumer’s acceptance. Despite great progress has been made in identification of the genes related to fruit softening, the upstream transcriptional regulatory pathways of these softening-related genes are not fully elucidated. Here, a novel bHLH gene, designated as MabHLH28, was identified because of its significant upregulation in banana fruit ripening. DAP-Seq analysis revealed that MabHLH28 bound to the core sequence of ‘CAYGTG’ presented in promoter regions of fruit softening-associated genes, such as the genes related to cell wall modification (MaPG3, MaPE1, MaPL5, MaPL8, MaEXP1, MaEXP2, MaEXPA2 and MaEXPA15) and starch degradation (MaGWD1 and MaLSF2), and these bindings were validated by EMSA and DLR assays. Transient overexpression and knockdown of MabHLH28 in banana fruit resulted in up- and down-regulation of softening-related genes, thereby hastening and postponing fruit ripening. Furthermore, overexpression of MabHLH28 in tomato accelerated the ripening process by elevating the accumulation of softening-associated genes. In addition, MabHLH28 showed interaction withMaWRKY49/111 and itself to form protein complexes, which could combinatorically strengthen the transcription of softening-associated genes. Taken together, our findings suggest that MabHLH28 mediates fruit softening by upregulating the expression of softening-related genes either alone or in combination with MaWRKY49/111.

show abstract

Section: Resultsmentioning

confidence: 99%