Balysum-2 inhibitis evoked activity of the pyramidal neurons in hippocampus

Abstract

The effects of Balysum-2 on the responsiveness of field CA1 hippocampal pyramidal neurons was studied in experiments with cultured cerebral slices. Addition to the medium of 10-*M Balysum-2 or its active ingredient (comenic acid) led to a reversible decrease in the peak amplitude of focal response (pop-spike) recorded in the pyramidal layer CA1 during stimulation of the radial layer. Perfusion of hippocampal slices with a solution containing the noncompetitive GABAA-antagonist picrotoxin prevented the effects of Balysum-2 and comenic acid. Inh~ition of hippocampal pyramids by Balysum-2 and comenic acid is probably caused by an increase in the inhibitory effect mediated by GABAA-receptors. Key Words: Balysum, comenic acid; hippocampal slices; GABAA-receptors; picrotoxinThe new preparation Balysum-2 has been widely used in medicine [6]. This nontoxic drug is an affective therapeutic tool against drug-resistant staphylococci in the treatment of numerous diseases [5]. Balysum exhibits antistressor, antioxidant, and the growth stimulation activities [3].The mechanisms underlying the effects of Balysum are practically unknown. It is not clear whether the central nerve system structures are sensitive to Balysum or whether its activity is limited to peripheral effects. To answer this question we studied the effect of Balysum-2 on evoked activity of hippocampal neuron. Hippocampus is the central structure that controls emotional behavior and visceral reactions [2]; it can be the target for drugs that modulate these processes. MATERIALS AND METHODSExperiments were carried out on hippocampal slices obtained from 3-6 week male Wistar rats [3]. The

Structure and Neuroprotector Properties of a Complex Compound of Lithium with Comenic Acid

“…The molecular structure of the comenate complex was studied using FT-IR spectrometry, 1 H and 13 C NMR spectroscopy, UV spectroscopy and thermal analysis and detailed via X-ray diffraction analysis. It should be noted that comenic acid is an active component of the drug baliz-2, used for cell regeneration in various pathologies [16,17]. Comenic acid reduces the toxic effect of glutamate on the culture of cerebellar neurons [18].…”

“…It should be noted that comenic acid is an active component of the drug baliz-2, used for cell regeneration in various pathologies [ 16 , 17 ]. Comenic acid reduces the toxic effect of glutamate on the culture of cerebellar neurons [ 18 ].…”

“…Regarding this class of compounds (Figure 1), there are data in the literature indicating a high therapeutic potential in preclinical studies [6][7][8][9][10][11][12][13][14][15]. It should be noted that comenic acid is an active component of the drug baliz-2, used for cell regeneration in various pathologies [16,17]. Comenic acid reduces the toxic effect of glutamate on the culture of cerebellar neurons [18].…”

Effects of potentiated antibodies to brain specific protein S100 on the dynamics of long-term potentiation in hippocampal slices

“…Long-term potentiation (LTP) in hippocampal slices represents a suitable model for studying molecular mechanisms of synaptic plasticity and testing the effects of biologically active substances on synaptic transmission [3,8,9]. Induction of LTP is a Ca2+-dependent process [2] controlled by Ca2 § proteins, including CaZ+-binding S 100 protein.…”