Native monoclonal antibodies to neurospecific S100 protein completely prevented the development of long-term potentiation (LTP) in rat hippocampal slices. Potentiated antiserum prepared by multiple dilutions according to homeopathic procedure (1:1012 ) did not affect LTP, but abolished the effect of native antiserum when applied 20 min prior to it. Neither nonimmune rabbit serum at the same dilution nor homeopathic solvent modified the development of LTP in hippocampal slices.
It was found that an increase in the excitability of command neurons of the reflex takes place during long-term sensitization (LTS). It was demonstrated that two factors are the cause of the increase in excitability: a depolarizational shift of the membrane potential of these cells (both a decrease in the initial resting potential and an accumulation of depolarization in the course of tactile stimulation), and a decrease in the threshold of generation of the action potentials. Injection of amphetamine leads to the appearance of sensitization, but its mechanisms only partially coincide with those in the case of LTS.
Antibodies to morphine produced after its chronic administration can contribute to changes in the central nervous system during opiate abuse. Facilitation of long-term posttetanic potentiation in mossy fibers of the hippocampus in rats with chronic morphine dependence can be reproduced in hippocampal slices from normal animals treated with antibodies to morphine. Incubation of hippocampal slices with ultralow doses of antibodies to morphine had no effect on control rats, but reduced facilitation of long-term potentiation in hippocampal slices from animals with chronic morphine dependence. This confirms the possibility of using ultralow doses of antibodies to morphine for therapeutic correction of mechanisms underlying the formation of drug abuse.
Incubation of hippocampal slices with antibodies to morphine did not change the total excitatory postsynaptic potential of mossy fibers, but markedly facilitated long-term posttetanic potentiation. Culturing of the organotypic hippocampal culture in the presence of 10 microM morphine increased the total excitatory postsynaptic potential of mossy fibers and reduced the probability of long-term posttetanic potentiation.
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