Balsalazine decreases intestinal mucosal permeability of dextran sulfate sodium-induced colitis in mice

Abstract

Aim:To investigate the effect of balsalazine treatment on intestinal mucosal permeability in dextran sulfate sodium (DSS)-induced colitis and to determine the mechanism of the balsalazine-induced changes. Methods: Experimental colitis was induced in C57BL/6J mice by the administration of 5% DSS. Balsalazine was administered intragastrically at doses of 42, 141, and 423 mg/kg. The disease activity index (DAI) score was evaluated and colon tissue was collected for the assessment of histological changes. The amount of malondialdehyde (MDA) in the colon was determined, along with the activity of myeloperoxidase (MPO), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Mucosa from the small intestine was collected to determine the levels of tumor necrosis factor (TNF)-α and interferon (IFN)-γ. The mucosa was ultrastructurally examined with transmission electron microscopy and intestinal permeability was assayed using Evans blue. Results: Balsalazine was found to reduce the DAI score and the histological index (HI) score, decrease the MDA content and the activity of MPO, and increase the activity of SOD and GSH-Px in colitis mice. At the same time, balsalazine ameliorated microvillus and tight junction structure, resulting in a decrease in the amount of Evans blue permeating into the intestinal wall and the levels of TNF-α and IFN-γ in colitis mice. Conclusion: In colitis mice, the anti-colitis effect of balsalazine results in a decrease in intestinal mucosal permeability. The mechanism of this effect is partly associated with balsalazine's antioxidative and anti-inflammatory effects.

Regulation of Obesity-Related Insulin Resistance with Gut Anti-inflammatory Agents

“…Treatment with gut anti-inflammatory agents, including 5-ASA and Balsalazide, has beneficial effects on the intactness of the gut epithelial barrier in IBD models (Di Paolo et al, 1996;Liu et al, 2009). We observed similar beneficial effects of 5-ASA on gut barrier function during HFD feeding, which are linked to reduced levels of inflammatory cytokines, such as TNF-a and IFNg, that can directly worsen barrier leakage of gut bacteria (Barreau et al, 2010;Beaurepaire et al, 2009).…”

Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)

“…Tight junctions form a seal between cells that are also important for intestine integrity in mice [24]. In vitro, down-regulation of occludin gene blocked the formation of the paracellular seal in human epidermal keratinocytes [73].…”

“…In addition to the antioxidant capacity, tight junction complexes are also critical in maintaining the structural integrity of intestine mice [24]. Claudin and occludin are the major integral membrane proteins of the mammalian tight junction that control the paracellular space between epithelial cells, thus preventing the paracellular diffusion of intestinal bacteria and other antigens across the epithelium [25,26].…”

Efeitos do butirato nos níveis de peroxidação lipídica em células da mucosa cólica sem trânsito fecal: estudo experimental em ratos

“…Os RLO em contato com as membranas celulares são capazes de oxidar fosfolípedes, destruindo as membranas celulares e consequentemente induzindo a apoptose celular 25 . O MDA é um dos metabólitos resultantes da lipoperoxidação das membranas e a sua dosagem tecidual vem sendo utilizada como potencial marcador de estresse oxidativo, em modelos de colite quimicamente induzida [39][40][41] . Do melhor do nosso conhecimento, apenas um único estudo avaliou, em modelos de CE, os níveis de MDA na mucosa exclusa de trânsito exposta à H 2 O 2 , substância capaz de formar altos níveis de RLO 27 .…”