Bajo polimorfismo en el sistema de antígenos de leucocitos humanos en población mestiza colombiana

Ávila-Portillo, Luz Mábel; Carmona, Alejandra; Franco, Leidy; Briceño, Ignacio; Casas, Maria C.; Gómez, Alberto

doi:10.11144/javeriana.umed51-4.bpsa

Cited by 5 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our statistical analyses showed that the extended haplotype hla-a*24-b*35-drb1*04 was the most frequent among donor and recipient (table 3) and that the haplotypes hla-a*24-b*35, hla-b*35-drb1*04 and hlaa*24-drb1*04 as well as the alleles hla-a*24, hla-b*35 and hla-drb1*04 were also highly frequent in the two groups (table 1 and 2). These results are in agreement with previous reports in Colombian (22)(23)(24) and other Latin American populations (10) where these alleles and haplotypes are the most frequently found.…”

Section: Discussionsupporting

confidence: 94%

Compatibilidad HLA donante-receptor y probabilidades de trasplante renal en una población colombiana

et al. 2016

View full text Add to dashboard Cite

Introduction: In Colombia, despite the fact that kidney transplants are the most common type of transplant surgery, a great number of transplanted patients do not achieve the desired Human Leucocyte Antigen (hla) compatibility. hla compatibility plays an important role in graft survival; patients with matched-hla have a lower chance of graft-versus-host disease and graft rejection. Objective: To determine the probability of finding an hla-matched donor-recipient pairs according to hla−a, −b and −drb1 frequencies in a specific Colombian population. Materials and methods: The study included a total of 484 unrelated individuals (61 donors and 423 recipients) from the hla registry. hla alleles were determined by polymerase chain reaction sequence with specific indicators. Results: hla-A*02,-A*24,-B*35 and-DRB1*04 alleles showed the highest minimum allele frequency (>10 %). In addition, hla-A*24-B*35-DRB1*04 was the most frequent extended haplotype in both donors and recipients (7.38 % and 6.76 %, respectively). Our experimental evidence showed that the maximum chance of finding at least one hla allele-matched kidney is 20.3 % for a patient with the most frequent extended haplotype, whereas for patients with rare or non-common haplotypes this probability is rather unlikely. Discussion: In terms of probability, the chance of finding an hla matched kidney donor/recipients in our region is low. This is due, at least in part, to the higher number of alleles and a the lower donation rate. Therefore, to define the hla profile of a population is important for establishing transplantation programs and alternative strategies in the kidney donation and allocation processes.

show abstract

Section: Discussionsupporting

confidence: 94%

Compatibilidad HLA donante-receptor y probabilidades de trasplante renal en una población colombiana

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Some 15-mer peptide sequences were selected by the following criteria: i) sequences with %Rank≥2 (strong binders) for at least two out of three prediction tools for more than one HLA-DR allele evaluated; ii) identical or similar sequences (synonym substitutions) between L. panamensis, L. braziliensis, and L. major species; and iii) sequences different from their human homolog when applicable. HLA-DR alleles taken in these predictions were based on studies of the most frequent alleles in the Colombian population (Trachtenberg et al, 1996;Correa et al, 2002;Reyes et al, 2007;Arias-Murillo et al, 2010;Ávila-Portillo et al, 2010;Yunis et al, 2013).…”

Section: In Silico Analysis For T Epitopesmentioning

confidence: 99%

Leishmania spp Epitopes in Humans Naturally Resistant to the Disease: Working Toward a Synthetic Vaccine

Flórez

Rodríguez

Cabrera³

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Vaccines are one of the most effective strategies to fight infectious diseases. Reverse vaccinology strategies provide tools to perform in silico screening and a rational selection of potential candidates on a large scale before reaching in vitro and in vivo evaluations. Leishmania infection in humans produces clinical symptoms in some individuals, while another part of the population is naturally resistant (asymptomatic course) to the disease, and therefore their immune response controls parasite replication. By the identification of epitopes directly in humans, especially in those resistant to the disease, the probabilities of designing an effective vaccine are higher. The aim of this work was the identification of Leishmania epitopes in resistant humans. To achieve that, 11 peptide sequences (from Leishmania antigenic proteins) were selected using epitope prediction tools, and then, peripheral blood mononuclear cells (PBMCs) were isolated from human volunteers who were previously divided into four clinical groups: susceptible, resistant, exposed and not exposed to the parasite. The induction of inflammatory cytokines and lymphoproliferation was assessed using monocyte-derived dendritic cells (moDCs) as antigen-presenting cells (APCs). The response was evaluated after exposing volunteers’ cells to each peptide. As a result, we learned that STI41 and STI46 peptides induced IL-8 and IL-12 in moDCs and lymphoproliferation and low levels of IL-10 in lymphocytes differentially in resistant volunteers, similar behavior to that observed in those individuals to L. panamensis lysate antigens. We conclude that, in silico analysis allowed for the identification of natural Leishmania epitopes in humans, and also STI41 and STI46 peptides could be epitopes that lead to a cellular immune response directed at parasite control.

show abstract

Using next‐generation sequencing for characterising HLA‐DRB1 and DQB1 loci in a cohort of Colombian women

Río‐Ospina

Camargo

Leon

et al. 2019

HLA

View full text Add to dashboard Cite

The Colombian population is characterised by a high genetic diversity, secondary to the ethnic mixture arising from colonisation. Unfortunately, few reports are available regarding HLA‐DRB1 and DQB1 diversity in Colombia to date. HLA‐DRB1 and DQB1 diversity was identified in this study using next‐generating sequencing (NGS) on a cohort of Colombian women. Cervical samples taken from 276 women were used for typing DRB1 and DQB1 loci by Illumina MiSeq. Allele and haplotype frequencies were calculated using an expectation‐maximisation algorithm. Hardy‐Weinberg Equilibrium and linkage disequilibrium (LD) between loci were evaluated. Forty‐seven DRB1 alleles and 14 DQB1 alleles were identified. DRB1*04:07:01G and DQB1*03:02:01G alleles occurred most frequently in the target population. Significant LD was found in 44 out of the 144 identified haplotypes, within which DRB1*04:07:01G‐DQB1*03:02:01G occurred most frequently (6.56%). The alleles and haplotypes found with NGS agreed with that found in previous reports involving lower resolution for the Colombian population, and greater genetic variability was found, especially concerning DRB1. Comparing allele and haplotype frequency distribution in the target population to that of other populations denoted HLA system intra‐ and inter‐population diversity.

show abstract

Bajo polimorfismo en el sistema de antígenos de leucocitos humanos en población mestiza colombiana

Cited by 5 publications

References 15 publications

Compatibilidad HLA donante-receptor y probabilidades de trasplante renal en una población colombiana

Compatibilidad HLA donante-receptor y probabilidades de trasplante renal en una población colombiana

Leishmania spp Epitopes in Humans Naturally Resistant to the Disease: Working Toward a Synthetic Vaccine

Using next‐generation sequencing for characterising HLA‐DRB1 and DQB1 loci in a cohort of Colombian women

Contact Info

Product

Resources

About