Bait spray for control of European cherry fruit fly: an appraisal based on semi‐field and field studies

Böckmann, Elias; Köppler, Kirsten; Hummel, Edmund; Vogt, Heidrun

doi:10.1002/ps.3621

Cited by 18 publications

(10 citation statements)

References 38 publications

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“…Currently, the control tactic available for A. fraterculus is the application of bait sprays (Dias et al, 2018). However, the control of SA fruit fly by chemical tactics is becoming increasingly restricted, because the effective but broad-spectrum neurotoxic and systemic-acting insecticides have been banned for commercialization (Böckmann et al, 2014). Fruit growers are also seeking new economic fruit fly control options, especially environmentally sustainable tactics (Sarles et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

The South American Fruit Fly: An Important Pest Insect With RNAi-Sensitive Larval Stages

et al. 2019

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RNA interference (RNAi) technology has been used in the development of approaches for pest control. The presence of some essential genes, the so-called “core genes,” in the RNAi machinery is crucial for its efficiency and robust response in gene silencing. Thus, our study was designed to examine whether the RNAi machinery is functional in the South American (SA) fruit fly Anastrepha fraterculus (Diptera: Tephritidae) and whether the sensitivity to the uptake of double-stranded RNA (dsRNA) could generate an RNAi response in this fruit fly species. To prepare a transcriptome database of the SA fruit fly, total RNA was extracted from all the life stages for later cDNA synthesis and Illumina sequencing. After the de novo transcriptome assembly and gene annotation, the transcriptome was screened for RNAi pathway genes, as well as the duplication or loss of genes and novel target genes to dsRNA delivery bioassays. The dsRNA delivery assay by soaking was performed in larvae to evaluate the gene-silencing of V-ATPase , and the upregulation of Dicer-2 and Argonaute-2 after dsRNA delivery was analyzed to verify the activation of siRNAi machinery. We tested the stability of dsRNA using dsGFP with an in vitro incubation of larvae body fluid (hemolymph). We identified 55 genes related to the RNAi machinery with duplication and loss for some genes and selected 143 different target genes related to biological processes involved in post-embryonic growth/development and reproduction of A. fraterculus . Larvae soaked in dsRNA (dsV-ATPase) solution showed a strong knockdown of V-ATPase after 48 h, and the expression of Dicer-2 and Argonaute-2 responded with an increase upon the exposure to dsRNA. Our data demonstrated the existence of a functional RNAi machinery in the SA fruit fly, and we present an easy and robust physiological bioassay with the larval stages that can further be used for screening of target genes at in vivo organisms’ level for RNAi-based control of fruit fly pests. This is the first study that provides evidence of a functional siRNA machinery in the SA fruit fly.

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Section: Introductionmentioning

confidence: 99%

The South American Fruit Fly: An Important Pest Insect With RNAi-Sensitive Larval Stages

et al. 2019

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“…Therefore, the detection of first flight becomes increasingly important to schedule precise insecticide applications. New methods have recently been developed for organic agriculture, such as biocontrol using the entomopathogenic fungus, Beauveria bassiana [ 8 ], or bait sprays with spinosad or Neem [ 9 , 10 , 11 ]. These methods also require an exact determination of the first flight.…”

Section: Introductionmentioning

confidence: 99%

A New Visual Trap for Rhagoletis cerasi (L.) (Diptera: Tephritidae)

Daniel

Mathis²,

Feichtinger³

2014

Insects

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The European cherry fruit fly, Rhagoletis cerasi (L.) (Diptera: Tephritidae), is the most important pest of sweet cherries in Europe. The aim of our experiments was to develop a new, cost-efficient, lead chromate-free and more eco-friendly trap for monitoring and mass trapping of R. cerasi. Five different-colored yellow panels and three different trap shapes were compared to a standard Rebell® amarillo trap in three experimental orchards in 2012. Trap color F, with a strong increase in reflectance at 500–550 nm and a secondary peak in the UV-region at 300–400 nm, captured significantly more flies than the standard Rebell® amarillo trap. Yellow traps with increased reflectance in the blue region (400–500 nm) were least attractive. Trap shape was of minor importance, as long as the object was three-dimensional and visible from all directions. Based on economic and practical considerations, a cylinder-shaped trap “UFA-Samen Kirschenfliegenfalle” was developed for commercial use and is currently under on-farm evaluation.

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“…The European cherry fruit fly is known for its intimate association with the host tree and its overall mobility largely restricted to the local canopy and its close neighborhood (Böckmann et al, 2012, 2014; Daniel and Grunder, 2012; Daniel and Baker, 2013). Based on our preliminary on-farm observations, the average area covered during a single local exploration errand was estimated at 100 sqm, which was used to determine the basic sector size (10 × 10 m) for all the arrays representing various aspects of the farm, and consequently—the spatial resolution of the whole simulation process.…”

Section: Resultsmentioning

confidence: 99%

Validation of Individual-Based Markov-Like Stochastic Process Model of Insect Behavior and a “Virtual Farm” Concept for Enhancement of Site-Specific IPM

et al. 2016

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The paper reports application of a Markov-like stochastic process agent-based model and a "virtual farm" concept for enhancement of site-specific Integrated Pest Management. Conceptually, the model represents a "bottom-up ethological" approach and emulates behavior of the "primary IPM actors"-large cohorts of individual insects-within seasonally changing mosaics of spatiotemporally complex faming landscape, under the challenge of the local IPM actions. Algorithms of the proprietary PESTonFARM model were adjusted to reflect behavior and ecology of R. cerasi. Model parametrization was based on compiled published information about R. cerasi and the results of auxiliary on-farm experiments. The experiments were conducted on sweet cherry farms located in Austria, Germany, and Belgium. For each farm, a customized model-module was prepared, reflecting its spatiotemporal features. Historical data about pest monitoring, IPM treatments and fruit infestation were used to specify the model assumptions and calibrate it further. Finally, for each of the farms, virtual IPM experiments were simulated and the model-generated results were compared with the results of the real experiments conducted on the same farms. Implications of the findings for broader applicability of the model and the "virtual farm" approach-were discussed.

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Bait spray for control of European cherry fruit fly: an appraisal based on semi‐field and field studies

Abstract: Bait sprays containing neem are a promising alternative for the management of R. cerasi, especially where the risk of immigration of fertilized females is low, as in isolated orchards or as part of area-wide treatments.

Cited by 18 publications

References 38 publications

The South American Fruit Fly: An Important Pest Insect With RNAi-Sensitive Larval Stages

The South American Fruit Fly: An Important Pest Insect With RNAi-Sensitive Larval Stages

A New Visual Trap for Rhagoletis cerasi (L.) (Diptera: Tephritidae)

Validation of Individual-Based Markov-Like Stochastic Process Model of Insect Behavior and a “Virtual Farm” Concept for Enhancement of Site-Specific IPM

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