Baicalin attenuates proinflammatory cytokine production in oxygen–glucose deprived challenged rat microglial cells by inhibiting TLR4 signaling pathway

Hou, Jincai; Wang, Jun; Zhang, Peng; Li, Dan; Zhang, Cuixiang; Zhao, Haiping; Fu, Jianhua; Wang, Bing; Liu, Jianxun

doi:10.1016/j.intimp.2012.10.013

Cited by 47 publications

(33 citation statements)

References 50 publications

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“…Regardless of whether it is in the heart [27], the brain [28][29][30], the lungs [31], the kidneys [32], the liver [33], or the pancreas [34], baicalin has a significant inhibitory effect as long as there are ligand-activated TLRs present, and the expression of downstream inflammatory cytokines is induced. Several laboratories have independently verified that baicalin can inhibit TLR2/4 expression induced by endogenous or exogenous ligands, inhibit NF-κB transfer to the nucleus, and reduce TNF-α expression.…”

Section: Discussionmentioning

confidence: 99%

The effects of baicalin on the TLR2/4 signaling pathway in the peripheral blood mononuclear cells of a lipopolysaccharide-induced rat fever model

Tao

Wang

et al. 2015

International Immunopharmacology

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Section: Discussionmentioning

confidence: 99%

The effects of baicalin on the TLR2/4 signaling pathway in the peripheral blood mononuclear cells of a lipopolysaccharide-induced rat fever model

Tao

Wang

et al. 2015

International Immunopharmacology

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“…It is considered to be one of the effective and safe drugs widely used in Asia for the treatment of a variety of diseases, such as brain diseases, hepatic disorders, inflammatory diseases, and so on. Furthermore, it has been reported that baicalin has multiple biologic functions, including anti-inflammatory, antioxidant, antiapoptotic, and immune regulation properties (Xu et al, 2011;Yin et al, 2011;Hou et al, 2012). There is increasing evidence to support the notion that this compound may have potential roles in anti-inflammation and immune regulation (Liu et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Baicalin on Collagen-Induced Arthritis in Rats through the Nuclear Factor–κB Pathway

Wang

Huang

et al. 2014

J Pharmacol Exp Ther

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This study focused on the potential therapeutic effect of baicalin on collagen-induced arthritis (CIA) in rats and the underlying mechanisms. The CIA rats were injected with baicalin (50, 100, or 200 mg/kg) once daily for 30 days. The rats were monitored for clinical severity of arthritis, and joint tissues were used for radiographic assessment and histologic examination. We quantified tumor necrosis factor-a (TNF-a) and interleukin-1b (IL-1b) in experimental animals and used Western blots to assess levels of protein abundance, phosphorylation, and acetylation of nuclear factor (NF)-kB p65 and sirtuin 1 (sirt1) protein expression in joint tissues. Human fibroblast-like synoviocytes from rheumatoid arthritis (HFLS-RA) were adopted in further mechanistic investigations. Baicalin intraperitoneal injection for 30 days dose-dependently blocked clinical manifestations of CIA, such as functional impairment and swollen red paws. Meanwhile, it alleviated collageninduced joint inflammation injury and inhibited the secretion of TNF-a and IL-1b in both rat synovium and HFLS-RA. Further mechanistic investigations revealed that baicalin suppresses NF-kB p65 protein expression and phosphorylation in synovial tissue and human-derived synoviocytes. Moreover, the acetylation of NF-kB p65 was downregulated by baicalin, which negatively correlates with the baicalin-induced upregulation of sirt1 expression in the same conditions. The data indicate that CIA in rats can be alleviated by baicalin treatment via relieving joint inflammation, which is related to the suppression of synovial NF-kB p65 protein expression and the elevation of its deacetylation by sirt1.

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“…As summarized in Table 1, It is reported that Ber, Bai and Rhe exert inhibitory effect on TLR-4 expression in varies of models (Lee et al, 2010; Li et al, 2011; Cabrera-Benitez et al, 2012; Hou et al, 2012; Chen C. C. et al, 2014; Chen et al, 2015), and the combination of TLR-4 and LPS is observed to be blocked by Ber (Jeong et al, 2014). So, it seems that the anti-inflammatory mechanism of SHXXT begins at a really early stage, ever since LPS are interacting with upstream membrane protein.…”

Section: Pharmacodynamic Levelmentioning

confidence: 99%

“…Among them, MyD88 has been most systemically studied both in vivo and in vitro . In respect of these adaptor molecules, Ber and Bai negatively regulate their protein expressions (Pandey et al, 2008; Hou et al, 2012; Lim et al, 2012; Chen C. C. et al, 2014; Feng et al, 2014; Wan et al, 2014), however the main constituents from RR are rarely mentioned.…”

Section: Pharmacodynamic Levelmentioning

confidence: 99%

San-Huang-Xie-Xin-Tang Constituents Exert Drug-Drug Interaction of Mutual Reinforcement at Both Pharmacodynamics and Pharmacokinetic Level: A Review

et al. 2016

Front. Pharmacol.

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Inflammatory disorders underlie varieties of human diseases. San-Huang-Xie-xin-Tang (SHXXT), composed with Rhizoma Rhei (Rheum palmatum L.), Rhizoma Coptidis (Coptis chinensis Franch), and Radix Scutellaria (Scutellaria baicalensis Georgi), is a famous formula which has been widely used in the fight against inflammatory abnormalities. Mutual reinforcement is one of the basic theories of traditional Chinese medicine. Here this article reviewed and analyzed the recent research on (1) How the main constituents of SHXXT impact on inflammation-associated signaling pathway molecules. (2) The interaction between the main constituents and efflux pumps or intestinal transporters. The goal of this work was to, (1) Provide evidence to support the theory of mutual reinforcement. (2) Clarify the key targets of SHXXT and suggest which targets need further investigation. (3) Give advice for the clinical use of SHXXT to elevated the absorption of main constituents and eventually promote oral bioavailability. We search literatures in scientific databases with key words of “each main SHXXT constituent,” in combination with “each main inflammatory pathway target molecule” or each main intestinal transporter, respectively. We report the effect of five main constituents on target molecules which lies in three main inflammatory signaling pathways, we as well investigate the interaction between constituents and intestinal transporter. We conclude, (1) The synergistic effect of constituents at both levels confirm the mutual reinforcement theory of TCM as it is proven in this work. (2) The effect of main constituents on downstream targets in nuclear need more further investigation. (3) Drug elevating the absorption of rhein, berberine and baicalein can be employed to promote oral bioavailability of SHXXT.

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Baicalin attenuates proinflammatory cytokine production in oxygen–glucose deprived challenged rat microglial cells by inhibiting TLR4 signaling pathway

Cited by 47 publications

References 50 publications

The effects of baicalin on the TLR2/4 signaling pathway in the peripheral blood mononuclear cells of a lipopolysaccharide-induced rat fever model

The effects of baicalin on the TLR2/4 signaling pathway in the peripheral blood mononuclear cells of a lipopolysaccharide-induced rat fever model

Inhibitory Effect of Baicalin on Collagen-Induced Arthritis in Rats through the Nuclear Factor–κB Pathway

San-Huang-Xie-Xin-Tang Constituents Exert Drug-Drug Interaction of Mutual Reinforcement at Both Pharmacodynamics and Pharmacokinetic Level: A Review

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