Baicalin alleviated APEC-induced acute lung injury in chicken by inhibiting NF-κB pathway activation

Peng, Lu-Yuan; Yuan, Meng; Song, Ke; Yu, Jialin; Li, Jinghe; Huang, Jiang-Ni; Yi, Pengfei; Fu, Binying; Shen, Haiqing

doi:10.1016/j.intimp.2019.04.046

Cited by 39 publications

(18 citation statements)

References 27 publications

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“… Compounds Structure In vitro/in vivo Model (effective dose) Cells (effective concentration) Related pharmacological indicators Related molecular mechanisms Refs. Luteolin 1 LPS/CLP (1 mg/kg) RAW 264.7 (20 μM) MPO↓ TNF-α↓ IL1-β↓ IL-6↓ MDA↓ SOD↑ CAT↑ GSH↑ COX-2↓ iNOS↓ ICAM-1↓ HMGB1↓ Inhibition of PI3K/Akt-mediated NF-κB and MAPK signaling pathways Activation of ERK1/2- and Ca 2+ -dependent HO-1 induction [ [65] , [66] , [67] , [68] , [69] , [70] , [71] , [72] , [73] ] Baicalein 2 CLP/I/R (10 mg/kg) MPMs (2.5 μM) MPO↓ TNF-α↓ IL1-β↓ IL-6↓ ICAM-1↓ IL-12↓ MCP-1↓ Inhibition of TLR4-MD2- MAPKs/ NF-κB signaling pathways Upregulation of Nrf2/HO-1 signaling pathway Inhibition of Bax/Bcl-2-mediated apoptosis [ [74] , [75] , [76] ] Baicalin 3 LPS/ Burn/RSV/IAV/APEC/ SiO 2 /Air embolism/Paraquat /Cigarette smoke/Pancreas (20 mg/kg) MPO↓ TNF-α↓ IL1-β↓ IL-6↓ IL-8↓IL-23↓ MDA↓ SOD↑ CAT↑ IL-18↓ TGF-β↓ HMGB1↓ Activation of Nrf2/HO-1 signaling pathway Inhibition of NLRP3 inflammasome Inhibition of the crosstalk between CX3CL1-CX3CR1 axis and NF-κB pathway Up-regulation of autophagy Inhibition of PI3K/AKT/ NF-κB signaling pathway [ [77] , [78] , [79] , [80] , [81] , [82] , [83] , [84] , [85] , [86] , [87] ] Tanshinone IIA 4 LPS/Paraquat/Blast/Pancreatitis /Seawater...…”

Section: Mechanisms Involved In Ali/ardsmentioning

confidence: 99%

“…Baicalin 3 , also one of the principal constituents extracted from Chinese medicinal plant Scutellaria baicalensis Georgi, can alleviate lung impairment as shown by attenuated MPO activity, lung edema and lung histopathologic changes in ALI models induced by various stimuli such as LPS, burn, respiratory syncytial virus (RSV), Influenza A virus (IAV), SiO 2 , air embolism, paraquat, cigarette smoke or pancreatitis [ [77] , [78] , [79] , [80] , [81] , [82] , [83] , [84] , [85] ]. Baicalin could alleviate pulmonary inflammation through down-regulating expression of pro-inflammatory cytokines TNF-a, IL-1β, IL-6, IL-8, IL-18, IL-23 and MMP9 [ 77 ].…”

Section: Natural Compounds That Exert Anti-ali Effectsmentioning

confidence: 99%

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Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

Zhou

et al. 2021

Pharmacological Research

189

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Section: Mechanisms Involved In Ali/ardsmentioning

confidence: 99%

Section: Natural Compounds That Exert Anti-ali Effectsmentioning

confidence: 99%

Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

Zhou

et al. 2021

Pharmacological Research

189

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“…44 In some previous studies, E. coli-derived LPS were used to induce the model of acute lung injury in chickens. 37,44,45 In the current experiment, LPS treatment in the positive control group clearly stimulated a diffuse inflammatory response, severe hemorrhage, and thicker interatrial septum, which resulted in higher ALI scores in the positive control group as compared with those in the negative control group. Ansari et al 44 found indistinguishable margins between different parts of the pulmonary lobules, along with a narrowed lumen of the pulmonary atria and obvious congestion and heavy leukocytes infiltration of the pulmonary parenchyma 12 hour after LPS inoculation to broiler chickens.…”

Section: Discussionmentioning

confidence: 46%

<p>Comparative Effects of Dexamethasone and Meloxicam on Magnitude of the Acute Inflammatory Response Induced by <em>Escherichia coli</em> Lipopolysaccharide in Broiler Chickens</p>

Tavakoli¹,

Abdi-Hachesoo²,

Nazifi³

et al. 2020

JIR

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Purpose: Dexamethasone has been widely used to treat acute inflammatory diseases and endotoxic shocks in animal models. Meloxicam is one of the most commonly used antiinflammatory agents in avian species. However, little is known about the effects of dexamethasone and meloxicam on lipopolysaccharide (LPS)-induced acute inflammatory response in birds. In the present study, LPS-challenged broiler chickens were used to investigate the comparative protective effects of meloxicam and dexamethasone on LPSinduced acute inflammatory responses. Methods: Lipopolysaccharide (LPS)-induced acute lung injury (ALI) histopathological scores, selected serum acute phase reactants, inflammatory mediators, and gangliosides were evaluated in broiler chickens inoculated with E. coli LPS and simultaneously treated with two doses of meloxicam (0.5 and 2 mg/kg BW) and dexamethasone (2 and 4 mg/kg BW). Results: LPS-induced ALI scores were not significantly different between the meloxicamtreated, dexamethasone-treated, and untreated positive control groups at 4 hours after LPS inoculation. Interleukin-6 concentrations were also statistically the same among the positive control, dexamethasone-treated, and meloxicam-treated groups at 3 and 12 hours after LPS inoculation. However, these anti-inflammatory drugs reduced adenosine deaminase, ceruloplasmin, lipid-bound sialic acid, protein-bound sialic acid, and total sialic acid in LPS-inoculated broiler chickens at 12, 24, and 48 hours after LPS inoculation in a drug-and dose-dependent manner. Ovotransferrin concentrations were not significantly different between positive control and treatment groups at 12 hours after LPS inoculation. However, twenty-four hours after LPS inoculation, all the treated groups, except the one treated with 0.5 mg/kg meloxicam, showed significantly lower concentrations of ovotransferrin as compared with the positive control group. Conclusion: Our results showed that dexamethasone was more effective than meloxicam in inhibiting the LPS-induced response in broiler chickens by diminishing the serum levels of adenosine deaminase, ceruloplasmin, and gangliosides.

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“…Immune cells in the lung such as alveolar macrophages and PMNs further cause a proinflammatory microenvironment and aggravate lung injury ( Mantovani et al, 2019 ). The superfluous recruitment of PMNs into lung tissues is a hallmark of ALI ( Peng et al, 2019 ; Ye S. et al, 2020 ). MPO is an enzyme present only in PMNs and involves oxidative stress ( Ye S. et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Glycoproteins From Rabdosia japonica var. glaucocalyx Regulate Macrophage Polarization and Alleviate Lipopolysaccharide-Induced Acute Lung Injury in Mice via TLR4/NF-κB Pathway

et al. 2021

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Background and Aims:Rabdosia japonica var. glaucocalyx is a traditional Chinese medicine (TCM) for various inflammatory diseases. This present work aimed to investigate the protective effects of R. japonica var. glaucocalyx glycoproteins on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the potential mechanism.Methods: Glycoproteins (XPS) were isolated from R. japonica var. glaucocalyx, and homogeneous glycoprotein (XPS5-1) was purified from XPS. ANA-1 cells were used to observe the effect of glycoproteins on the secretion of inflammatory mediators by enzyme-linked immunosorbent assay (ELISA). Flow cytometry assay, immunofluorescence assay, and Western blot analysis were performed to detect macrophage polarization in vitro. The ALI model was induced by LPS via intratracheal instillation, and XPS (20, 40, and 80 mg/kg) was administered intragastrically 2 h later. The mechanisms of XPS against ALI were investigated by Western blot, ELISA, and immunohistochemistry.Results:In vitro, XPS and XPS5-1 downregulated LPS-induced proinflammatory mediators production including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and nitric oxide (NO) and upregulated LPS-induced IL-10 secretion. The LPS-stimulated macrophage polarization was also modulated from M1 to M2. In vivo, XPS maintained pulmonary histology with significantly reducing protein concentration and numbers of mononuclear cells in bronchoalveolar lavage fluid (BALF). The level of IL-10 in BALF was upregulated by XPS treatment. The level of cytokines including TNF-α, IL-1β, and IL-6 was downregulated. XPS also decreased infiltration of macrophages and polymorphonuclear leukocytes (PMNs) in lung. XPS suppressed the expression of key proteins in the TLR4/NF-κB signal pathway.Conclusion: XPS was demonstrated to be a potential agent for treating ALI. Our findings might provide evidence supporting the traditional application of R. japonica var. glaucocalyx in inflammation-linked diseases.

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Baicalin alleviated APEC-induced acute lung injury in chicken by inhibiting NF-κB pathway activation

Cited by 39 publications

References 27 publications

Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

<p>Comparative Effects of Dexamethasone and Meloxicam on Magnitude of the Acute Inflammatory Response Induced by <em>Escherichia coli</em> Lipopolysaccharide in Broiler Chickens</p>

Glycoproteins From Rabdosia japonica var. glaucocalyx Regulate Macrophage Polarization and Alleviate Lipopolysaccharide-Induced Acute Lung Injury in Mice via TLR4/NF-κB Pathway

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