Bacteriostatic effect of copaiba oil (Copaifera officinalis) against Streptococcus mutans

Pieri, Fábio Alessandro; Mussi, Maria Carolina Martins; Fiorini, João Evangelista; Moreira, Maria Aparecida Scatamburlo; Schneedorf, José Maurício

doi:10.1590/s0103-64402012000100006

Cited by 31 publications

(35 citation statements)

References 14 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a review of the fundamentals about black stains, its diagnosis and possible differential diagnoses, microbiology and therapy, Ronay and Attin (15) concluded that the reasons why the results are not clear about the black stains are related to the specific oral microflora of these stains. Another study (22) reinforced that chlorhexidine solution could cause some stains on teeth surface and confirms the great potential of using copaiba oil and chlorhexidine solution against the growth of S. mutans. The present study demonstrated that black pigmented bacteria (P. nigrescens and P. intermedia) as well as cariogenic non-black pigmented bacteria (S. mutans and Actinomyces spp) can be detected in dental biofilms of patients carrying black tooth stains or not.…”

Section: Discussionmentioning

confidence: 62%

Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans

et al. 2012

View full text Add to dashboard Cite

This study investigated the presence of the black-pigmented bacteria Prevotella nigrescens and Prevotella intermedia, the non-black-pigmented bacteria Actinomyces spp and particularly the cariogenic pathogen Streptococcus mutans in the dental biofilms of patients with or without black extrinsic tooth stains, using the multiplex polymerase chain reaction (PCR) technique. Analysis of the dental biofilms of patients with (n=26) or without (n=26) black tooth stains was performed using duplex PCR for the 16S ribosomal RNA gene (P. nigrescens, P. intermedia, Actinomyces spp) and glucosyltransferase-I gene for S. mutans. P. nigrescens and S. mutans were the most frequent bacteria detected in both groups. The least frequently detected were P. intermedia and Actinomyces spp. The similar bacterial composition of dental biofilms of black tooth stains and healthy tooth surfaces indicates that black tooth stains are not free of cariogenic bacteria.

show abstract

“…Used since the 16th century for medicinal purposes 2,6 , Copaíba oil is described in the literature as anti-edematous 5 , anti-inflammatory 3,7,8 , antibacterial 8,9 , insecticide 5,6,8 , antifungal 10 and wound healing 8,11 . It is popularly adopted in the treatment of several diseases, such as cystitis, bronchitis, chronic diarrhea, rheumatism, psoriasis, tumors 9 and gonorrhea 4 .…”

Section: Officinaliis C Reticulata C Langsdorffii C Guyanensismentioning

confidence: 99%

Comparative analysis of the effects of Copaifera multijuga oil-resin and nitrofurazona in the cutaneous wound healing process

Martini

Scapini

Collaço

et al. 2016

Rev. Col. Bras. Cir.

View full text Add to dashboard Cite

Objectives: to evaluate, histologically and macroscopically, the influence of Copaifera multijuga (Copaíba) oil-resin on the healing process of cutaneous wounds, comparing it with nitrofurazone. Methods: we divided 36 rats into three groups of 12 animals, according to the treatment to be administered. Group SL (control) received saline on the lesion; the Group OIL received topical treatment with Copaíba oil; and the Group NITRO was treated with Nitrofurazone. We inflicted a circular wound of 8mm in diameter on the back of each animal. We subdivided each of the three groups of 12 animals into three subgroups, according to treatment time and euthanasia (7, 14 and 21 days). All animals received the proposed treatment daily. We photographed the lesions for area measurement, as well as for evaluation of macroscopic aspects. We resected and stained the scars to quantify and qualify elastic fibers, collagen, degree of epithelization, neovascularization and inflammation. Results: although the saline solution provided a faster wound closure in its initial phase, after 14 days the wound size of the three groups tested was the same. Levels of inflammation and neovascularization were similar in all three groups. The amount of collagen and elastic fibers was higher in the Nitrofurazone and Copaíba oil groups. Conclusion: in male Wistar rats, Copaifera multijuga oil-resin positively influences the healing process, but it is less effective than nitrofurazone in healing by secondary intention.

show abstract

“…Mutans streptococci and lactobacilli are microorganisms with known cariogenic capacities (6)(7)(8). The first is considered an etiological agent of carious lesions and is able to produce (acidogenic) and tolerate (aciduric) high quantities of lactic acid, and the second is considered particularly involved in the progression of carious lesions (8,9).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Synergism of Lactoferrin and Lysozyme Against Cariogenic Microorganisms

Andrade

Oliveira²,

Yoshie³

et al. 2014

Braz. Dent. J.

View full text Add to dashboard Cite

The present study evaluated the antimicrobial in vitro effects of the salivary proteins lactoferrin and lysozyme on microorganisms involved in the carious process, obtaining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Streptococcus mutans (ATCC 25175) and Lactobacillus casei (ATCC 7469) were submitted to broth macrodilution of lysozyme at 80 mg/mL and lactoferrin at 200 mg/mL. The tubes were read in a spectrophotometer after they had been incubated at 37 °C for 18 h, in a carbon dioxide chamber, in order to read the MIC. A new subculture was carried on agar plates to obtain the MBC. The agar diffusion method was also tested, using BHI agar with 100 µL of the standardized microbial inocula. Filter-paper disks soaked in 10 µL of the solutions lactoferrin (200 µg/mL) and lysozyme (80 µg/mL) were placed on the agar surface. Inhibition halos were not observed on the plates, showing the absence of the antimicrobial effects of these proteins in this method. The bactericidal and bacteriostatic effects of lysozyme on L. casei were 50.3 mg/mL and 43.1 mg/mL respectively. The bactericidal and bacteriostatic effects on S. mutans were 68.5 mg/mL and 58.7 mg/mL. Lactoferrin did not induce any inhibitory effects on any microorganism, even in the concentration of 200 mg/mL. There was not a synergic antimicrobial effect of proteins, when they were tested together, even in the concentration of 42.8 mg/mL of lysozyme and 114 mg/mL of lactoferrin (the highest values evaluated). S. mutans and L. casei were only inhibited by lysozyme, not affected by lactoferrin and by the synergic use of both proteins.

show abstract

“…The present study and previous work by Packer and Luz (2007) showed similar results regarding the effects of copaiba oil on E. coli. However, Pieri et al (2012) suggested that copaiba oil exerts an antimicrobial action that is only bacteriostatic in nature, because no concentration led to 99.9% destruction of bacteria in his studies performed to determine the bacteriological concentration maximum. In addition to these microorganisms, copaiba oil has shown antimicrobial activity against cariogenic agents such as Streptococcus mutans and some biofilm-forming agents, such as Streptococcus salivarius, Streptococcus pyogenes and E. faecalis (Pieri et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial analysis of copaiba oil extract from Passiflora cincinnata and endodontic substances

Santos¹,

Leitao²,

Cavalcante³

et al. 2016

J. Microbiol. Antimicrob.

View full text Add to dashboard Cite

The aim of this study was to evaluate the antimicrobial activity and minimum inhibitory concentration (MIC) of copaiba oil, extracts of Passiflora cincinnata and substances commonly used against endodontic infections of bacterial strains certified by the American Type Culture Collection (ATCC) and against clinical isolates (CI). The methodology involved the preparation of crude extracts of the plants, the selection of copaiba oil and the standardization of samples. The antibacterial activity of these substances was tested against Enterococcus faecalis (ATCC), Escherichia coli (CI) and Pseudomonas aeruginosa (ATCC and CI). The MIC was determined by broth dilution and the nitroblue tetrazolium chloride dye reduction test. The data were statistically analyzed by the Kolmogorov-Smirnov normality test and by the Kruskal-Wallis test with a confidence level of 95%. The analysis of the antimicrobial activity showed that the ethanol extracts of P. cincinnata and the combination of calcium hydroxide with polyethylene glycol with and without camphorated monochlorophenol showed no antimicrobial activity. However, the copaiba oil and other substances evaluated showed some antimicrobial activity against the microorganisms used (p <0.01), exhibiting a MIC ranging from <0.3 to >400 µL/mL (p <0.05). Copaiba oil showed antimicrobial activity and could represent a potential phytotherapeutic agent to be used against microorganisms causing endodontic infections.

show abstract

“…In relation to antibacterial activity, the results were a promise regarding therapeutic aspects since the oil proved to inhibit bacterial samples at lower concentrations with attention to the action against S. saprophyticus (ATTC35552) by diffusion agar technique. Other reports indicated the in natura copaiba oil extract of identifi ed species such as C. reticulata (Oliveira et al, 2006;Santos et al, 2008), C. multijuga Hayne (Deus et al, 2011;Pieri et al, 2012) antimicrobial potential against Gram positive bacteria, including action against Methicillin-resistant Staphylococcus aureus (MRSA). However, these results indicate antimicrobial action, this copaiba oil effect seemed to be Gram positive restrain spectrum, since no action against Gram negative bacteria was reported.…”

Section: Discussionmentioning

confidence: 99%

Physicochemical and antimicrobial properties of copaiba oil: implications on product quality control

Fonseca

Barros

Apel

et al. 2015

Acta Sci Pol Technol Aliment

View full text Add to dashboard Cite

Background. The copaiba oil is a common natural product used in cosmetic industry and as a nutraceutical product. However, lack of quality control and scarce knowledge about its antimicrobial activity is a point of concern. The proposal of this study was to investigate the physicochemical properties and the antimicrobial activity of fi ve commercial brands of copaiba oil. Material and methods. Acidity and ester index, refractory index, solubility in alcohol, and thin layer chromatography were performed to verify the physicochemical properties of fi ve commercial copaiba oils sold in local pharmacies. Ultra performance liquid chromatography coupled with diode-array detection and electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-DAD/ESI-Q-TOF-MS) was used to investigate diterpene acids while the volatile compounds were analysed by gas chromatography-mass spectrometry (GC-MS). Antibacterial and antifungal activities were also evaluated by agar diffusion technique; and minimal inhibitory concentration and maximal bactericidal concentration were defi ned for each sample and bacteria. Results. The physical-chemical analysis revealed heterogeneity between all samples analysed. The A1 sample showed characteristics of copaiba oil and was mainly composed by hydrocarbon sesquiterpenes (29.95% β-bisabolene, 25.65% Z-α-bergamotene and 10.27% β-cariophyllene). Among diterpene acids, the UPLC-DAD/ESI-Q-TOF-MS data are compatible with presence of copalic and/or kolavenic acid (m/z 305 [M + H]

show abstract

Bacteriostatic effect of copaiba oil (Copaifera officinalis) against Streptococcus mutans

Cited by 31 publications

References 14 publications

Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans

Comparative analysis of the effects of Copaifera multijuga oil-resin and nitrofurazona in the cutaneous wound healing process

Antimicrobial Activity and Synergism of Lactoferrin and Lysozyme Against Cariogenic Microorganisms

Antimicrobial analysis of copaiba oil extract from Passiflora cincinnata and endodontic substances

Physicochemical and antimicrobial properties of copaiba oil: implications on product quality control

Contact Info

Product

Resources

About