Bacteriophages That Contain Lipid

Mindich, Leonard

doi:10.1007/978-1-4684-2724-0_5

Cited by 19 publications

(14 citation statements)

References 101 publications

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“…The degree of chloroform sensitivity of phage pilHor, like that of phage M, is less than that of suspensions of the lipid-containing phages belonging to the families Tecticiridae, Corticouiridae or Cystoviridae (Matthews, 1982), which are nearly totally inactivated under comparable conditions (Coetzee et al, 1979;Wong & Bryan, 1978;Bradley & Rutherford, 1975 ;Vidaver et al, 1973 ;Mindich, 1978). The RNA-containing phages also differ from phages of the above three families in that they are resistant to diethyl ether.…”

Section: J N C O E T Z E E a N D O T H E R Smentioning

confidence: 99%

Phage pilH : a Phage Which Adsorbs to IncHI and IncHII Plasmid-coded Pili

et al. 1985

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Phage pilHa was specific for bacterial strains, of various genera, harbouring plasmids of the HI and HII incompatibility groups. Plaque formation was temperature sensitive in that plaques formed at 26 °C but not at 37 °C. Plaques were fairly clear, irregular in outline and varied from pin point to about 2 mm in diameter on all hosts where plaques were detected. The phage had an isometric hexagonal outline with a diameter of 25 nm. It contained RNA but differed from all but one other plasmid-dependent RNA phage by being sensitive to chloroform. It adsorbed along the length of the shafts of IncHI and HII plasmid-coded pili.

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Section: J N C O E T Z E E a N D O T H E R Smentioning

confidence: 99%

Phage pilH : a Phage Which Adsorbs to IncHI and IncHII Plasmid-coded Pili

et al. 1985

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“…These include phages with long noncontractile tails (siphoviruses; Matthews 1982), such as the mutator phages D3112 (Krylov et al 1980(Krylov et al , 1982Rehmat and Shapiro 1983) and PH2 (Akhverdian et al 1984), the temperate polysaccharide-depolymerizing phage 2 (Castillo andBartell 1974, 1976), and the Pseudomonas pseudojlava phages of the gd series (Auling 1978;Auling et al 1980). Other well-characterized phages of this genus include the corticovirus, PM2 (Franklin 1978;Mindich 1978), the lipidcontaining cystovirus, 46 (Mindich 1978), and 4W-14 (Kropinski et al 1973), a member of the Myovindae. Although members of the family Podoviridae represent the second most commonly characterized phages of Pseudomonas only five have been characterized to any extent.…”

Section: Introductionmentioning

confidence: 96%

Isolation and characterization of a Pseudomonas aeruginosa bacteriophage with a very limited host range

Bigby

Kropinski²

1989

Can. J. Microbiol.

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A Pseudomonas aeruginosa bacteriophage, phi PLS743, with extremely limited host range has been isolated. It belongs to the virus family Podoviridae, morphological type C1, and possesses a head diameter of 45 nm. The phage has a buoyant density in CsCl of 1.516 g/cm3, and its mass is 45 x 10(6) daltons. The phage particles are composed of double-stranded DNA (49.9 mol% G + C; 42.4 kilobase pairs) and 11 structural proteins (66% by weight). The major head protein, P5, has a Mr of 34,500. The DNA is not cut by SalI or XhoI restriction endonucleases, but is cut by PvuII (1 site), KpnI and BglII (2 sites), PvuI (4 sites), BamHI (7 sites), EcoRI (9 sites), and HindIII (12 sites). A restriction endonuclease map is presented.

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“…Virions of this group appear to develop by the formation of an empty particle that eventually acquires DNA (8). This is similar to the apparent pathway for another lipid-containing phage, PM2 (6), and very different from the pathway demonstrated for 4)6, a lipid-containing phage that has a genome of doublestranded RNA and develops by the formation of a nucleocapsid that is finally covered by a lipidcontaining envelope (9). The PRD1 phages are much more resistant to organic solvents and detergents than 4)6 is, which is consistent with a layer of protein exterior to the lipid (12).…”

mentioning

confidence: 61%

Assembly of bacteriophage PRD1: particle formation with wild-type and mutant viruses

Mindich¹,

Bamford²,

McGraw³

et al. 1982

J Virol

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Bacteriophage PRD1 contains DNA, 17 proteins, and lipid. The assembly pathway involves the formation of empty particles that contain lipid and all of the proteins of mature virions, with the possible exception of one. The major and minor capsid proteins, P3 and P5, occur as soluble multimers before they appear in the empty particles. Nonsense mutants of PRD1 that involve structural proteins of the virion other than P3 form particles that are missing only the defective protein. Those mutants that are unable to form P3 do not form particles. Mutations in two other genes that code for nonstructural proteins (P10, which is membrane bound, and P17, which is soluble) result in the absence of particles. Protein P2 is necessary for adsorption to host cells. Protein P9 is necessary for particle filling with DNA, whereas P20 and P22 are necessary for stable DNA packaging. Electron micrographs of infected cells confirmed the gradient analysis of particle formation. No free vesicles were observed in mutants that could not form complete empty particles, indicating that there are no free intermediate particles before the empty virions.

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Bacteriophages That Contain Lipid

Cited by 19 publications

References 101 publications

Phage pilH : a Phage Which Adsorbs to IncHI and IncHII Plasmid-coded Pili

Phage pilH : a Phage Which Adsorbs to IncHI and IncHII Plasmid-coded Pili

Isolation and characterization of a Pseudomonas aeruginosa bacteriophage with a very limited host range

Assembly of bacteriophage PRD1: particle formation with wild-type and mutant viruses

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