Bacteriophage T4 nanoparticle capsid surface SOC and HOC bipartite display with enhanced classical swine fever virus immunogenicity: A powerful immunological approach

Wu, Jianmin; Tu, Changchun; Yu, Xun; Zhang, Maolin; Zhang, Nianzu; Zhao, Minyi; Nie, Wenxian; Ren, Zhaojun

doi:10.1016/j.jviromet.2006.09.017

Cited by 47 publications

(44 citation statements)

References 29 publications

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“…Either a single SOC or HOC site can be used to display large foreign molecular immunogens. Furthermore, the main advantages of the phage T4 system over other display technologies have been substantiated by using phage T4 SOC/HOC dual sites to display antigens (Wu et al 2007). The phage T4 HOC/SOC bipartite display system is attractive for the expression of cDNA, and the display of peptides or proteins at high copy number on the phage capsid surface.…”

Section: Phage Display Systemsmentioning

confidence: 99%

Phage display and its application in vaccine design

et al. 2010

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This review focuses on phage display and its application in vaccine design. Four kinds of phage display systems and their characteristics are highlighted. Whole phage particles can be used to deliver vaccines by fusing immunogenic peptides to modified coat proteins (phagedisplay vaccination), or by incorporating a eukaryotic promoter-driven vaccine gene within the phage genome (phage DNA vaccination). Hybrid phage vaccination results from a combination of phage-display and phage DNA vaccination strategies, indicating the potential for evolution of phage vaccines. Phage vaccines could provide a key to unlock new approaches in combating bacterial and viral pathogens, and cancer diseases. New phage display systems will certainly emerge because of the global abundance of phage and our increasing ability to exploit them. The scope of phage display applications will continue to expand. Over the ensuing period, research should be directed toward a better understanding of the immunization mechanisms involved in phage-mediated immunization, and the development of hybrid phage vaccination.

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Section: Phage Display Systemsmentioning

confidence: 99%

Phage display and its application in vaccine design

et al. 2010

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“…Enzyme-linked immunospot assay The enzyme-linked immunospot (ELISPOT) assay for the enumeration of the anti-Flt4 antibody-producing cells has been described [13] . Briefly, polyvinylidene difluoride (PVDF)-bottomed 96-well Filtration Plates (Millipore, Bedford, MA) were coated with 150 μL/mL T4-mFlt4 recombinant vaccine at 4°C overnight and then incubated at 37 °C for 1 h. Mononuclear cells prepared from spleen were enumerated from 1×10 6 /well and diluted 1:3 and then incubated on the plates at 37 °C for 5 h. Immunoglobulin G (IgG) bound to the membrane was revealed as spots with alkaline phosphatase-conjugated antimouse IgG antibodies.…”

Section: Immunogenicity Analysis By Western Blottingmentioning

confidence: 99%

“…Phage display is a powerful technique for identifying peptides or proteins that have desirable binding properties. First, the T4 phage display system is capable of displaying multiple copies of two different peptide/proteins and creating the "high concentration/density hot spot" of expressed protein to achieve its K d and simulate a relevant protein-protein interaction [13] . Second, the exogenous or syngeneic protein/peptide can be fused to both the N-and the C-termini of T4 SOC and HOC proteins at higher copy number than other filamentous phage systems.…”

Section: Introductionmentioning

confidence: 99%

“…Third, secretion of the fusion protein does not occur [14][15][16][17] . Moreover, T4 phage particles possess high antigenicity without adjuvant [13,18] . Finally, scientists have already conducted T4 phage safety testing of oral administration in humans and found it to be safe and accompanied by non-pathogenic side effects due to infection of enteric E coli [19] .…”

Section: Introductionmentioning

confidence: 99%

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Antitumor activity of endogenous mFlt4 displayed on a T4 phage nanoparticle surface

Ren

Zhao

et al. 2009

Acta Pharmacol Sin

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Aim: Flt4 plays a key role in promoting tumor metastasis by stimulating solid tumor lymphangiogenesis. In this study, mouse Flt4 (mFlt4) was displayed on T4 phage in order to explore the feasibility of breaking immune tolerance to "selfantigens" and to evaluate the phage's antitumor activity. Methods: A T4 phage nanometer particle expressing mFlt4 on the surface was constructed for evaluation as a recombinant vaccine. The presence of the mFlt4 gene in the T4-mFlt4 recombinant vaccine was verified by PCR and Western blot analysis. The immunotherapeutic potential of T4-mFlt4 was tested in mice injected with Lewis lung carcinoma (LLC) cells. AntiFlt4 antibody producing B cells were detected by ELISPOT. The effects of T4-mFlt4 on lymphatic metastasis and lymphangiogenesis were investigated in a mouse antimetastasis assay and by Flt4 and CD105 immunohistochemistry. Results: The T4-mFlt4 recombinant vaccine demonstrated antitumor activity and elicited autoantibodies against mFlt4. Mice carrying LLC-derived tumors exhibited prolonged survival when given the vaccine compared with control-treated animals. The vaccine also inhibited lymphangiogenesis and tumor metastasis in the mouse models. However, T4-mFlt4 was not observed to inhibit tumor growth. Conclusion: The T4-mFlt4 recombinant vaccine induced protective antitumor immunity and antimetastasis against LLC. Induction of an autoimmune response directed against tumor progression merits further study as a new strategy for immunotherapy in cancer.

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“…Such a library allows easy selection, which includes three steps: (i) incubating the molecule with the phage library, (ii) removing unbound phage particles, and (iii) amplifying the selected phages in E. coli cells (Paschke 2006). The phage-display technique has been used for many different purposes: identifying protein-ligand interactions, including screening for receptor agonists and antagonists, defining epitopes of monoclonal antibodies, selecting enzyme substrates or inhibitors, designing and improving enzyme properties, selecting targets for the inhibition of tumor-specific angiogenesis, combating infectious diseases, identifying peptide drug candidates, and for vaccine development (e.g., vvIBDV, CSFV, anthrax toxin, HIV, most of these discussed below) (Benhar 2001;Fernandez-Gacio et al 2003;Konthur 2002;Mullen et al 2006;Sergeeva et al 2006;Willats 2002;Wu et al 2006). This method has several advantages: the very high number of proteins that can be displayed, high flexibility, the selection may be performed in vitro and in vivo, in vitro selection may also involve inorganic molecules, and the technique is effective, fast, cheap, easy to control, and requires no special equipment (Willats 2002).…”

Section: Phage-display Systemmentioning

confidence: 99%

Molecular modification of T4 bacteriophage proteins and its potential application — Review

et al. 2009

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Bacteriophage T4 is a virus with well-known genetics, structure, and biology. Such techniques as X-ray crystallography, cryo-EM, and three-dimensional (3D) image reconstruction allowed describing its structure very precisely. The genome of this bacteriophage was completely sequenced, which opens the way for the use of many molecular techniques, such as site-specific mutagenesis, which was widely applied, e.g., in investigating the functions of some essential T4 proteins. The phage-display method, which is commonly applied in bacteriophage modifications, was successfully used to display antigens (PorA protein, VP2 protein of vvIBDV, and antigens of anthrax and HIV) on T4's capsid platform. As first studies showed, the phage-display system as well as site-specific mutagenesis may also be used to modify interactions between phage particles and mammalian cells or to obtain phages infecting species other than the host bacteria. These may be used, among others, in the constantly developing bacteriophage therapy. All manipulations of this popular bacteriophage may enable the development of vaccine technology, phage therapy, and other branches of biological and medical science.

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Bacteriophage T4 nanoparticle capsid surface SOC and HOC bipartite display with enhanced classical swine fever virus immunogenicity: A powerful immunological approach

Cited by 47 publications

References 29 publications

Phage display and its application in vaccine design

Phage display and its application in vaccine design

Antitumor activity of endogenous mFlt4 displayed on a T4 phage nanoparticle surface

Molecular modification of T4 bacteriophage proteins and its potential application — Review

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