Bacteriophage mediated control of necrotic enteritis caused by C. perfringens in broiler chickens.

Hosny, Reham A; A, Gaber; Sorour, Hend

doi:10.1007/s11259-021-09821-3

Cited by 7 publications

(6 citation statements)

References 35 publications

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“…After that, the phage lysate (10 8 PFUml −1 ) was tested using tryptic soy agar plates (Oxoid Limited, Thermo Fisher Scientific Inc.) by the spot test and plaque assay as described by Sorour et al ( 2020 ). Phage lysate was then purified three times, as described by Hosny et al ( 2021 ), using the plaque assay method with modification in the broth medium. A single clear plaque was picked into 300 μl of buffer peptone broth, followed by centrifugation and filtration as previously described in the amplification method.…”

Section: Methodsmentioning

confidence: 99%

Antibiofilm activity of a lytic Salmonella phage on different Salmonella enterica serovars isolated from broiler farms

et al. 2022

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Bacteriophages have been mainly used in treating infections caused by planktonic bacterial cells in the veterinary sector. However, their applications as antibiofilm agents have received little attention. Accordingly, a previously isolated Salmonella infecting Siphoviridae phage was investigated for host range against 15 Salmonella enterica isolates (S. Cape, S. Gallinarum, 4 S. Enteritidis, 3 S. Montevideo, S. Uno, S. Oritamerin, S. Belgdam, S. Agona, S. Daula, and S. Aba) recovered from the litters of commercial broiler farms. All S. enterica isolates were examined for their biofilm activity using a microtiter plate assay and for adrA, csgD, and gcpA genes using conventional PCR. The phage efficacy against established biofilms produced by the selected seven S. enterica isolates (S. Gallinarum, S. Enteritidis, S. Montevideo, S. Uno, S. Oritamerin, S. Belgdam, and S. Agona) was assessed using microtiter plate assay and reverse transcriptase real-time PCR over different incubation times of 5 and 24 h. All S. enterica isolates were strong biofilm formers. Moreover, the phage effectively reduced the biofilm activity of the established S. enterica biofilms in the microtiter plate assay using the independent sample t-test (P < 0.050). Furthermore, the relative expression levels of csgD, gcpA, and adrA genes in the biofilm cells of S. enterica isolate after phage treatment were significantly up-regulated to variable degrees using the independent sample t-test (P < 0.050). In conclusion, the present study revealed the potential use of Salmonella phage in reducing established biofilms produced by S. enterica serovars isolated from broiler farms.

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Section: Methodsmentioning

confidence: 99%

Antibiofilm activity of a lytic Salmonella phage on different Salmonella enterica serovars isolated from broiler farms

et al. 2022

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“…The administration of the polyvalent bacteriophage cocktail INT-401 in chickens effectively improves the weight gain and feed conversion ratio and reduces mortality by 92% during NE challenge [ 234 ]. Similarly, C. perfringens podovirus phage administration reduces the gross pathology, mortality, and cecal load of C. perfringens in infected birds [ 236 ]. Moreover, administration of bacteriophages at a concentration of 106–107 plaque-forming units significantly improves weight gain, feed efficiency, and decrease mortality in a NE challenge model.…”

Section: Control Of Necrotic Enteritis In the Post-antibiotic Eramentioning

confidence: 99%

Necrotic Enteritis in Broiler Chickens: A Review on the Pathogen, Pathogenesis, and Prevention

et al. 2022

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Clostridium perfringens type A and C are the primary etiological agents associated with necrotic enteritis (NE) in poultry. The predisposing factors implicated in the incidence of NE changes in the physical properties of the gut, the immunological status of birds, and the disruption of the gut microbial homeostasis, causing an over-proliferation of C. perfringens. The principal virulence factors contributing to the pathogenesis of NE are the α-toxin, β-toxin, and NetB toxin. The immune response to NE in poultry is mediated by the Th1 pathway or cytotoxic T-lymphocytes. C. perfringens type A and C are also pathogenic in humans, and hence are of public health significance. C. perfringens intoxications are the third most common bacterial foodborne disease after Salmonella and Campylobacter. The restrictions on the use of antibiotics led to an increased incidence of NE in poultry. Hence, it is essential to develop alternative strategies to keep the prevalence of NE under check. The control strategies rely principally on the positive modulation of host immune response, nutritional manipulation, and pathogen reduction. Current knowledge on the etiology, pathogenesis, predisposing factors, immune response, effect on the gut microbial homeostasis, and preventative strategies of NE in this post-antibiotic era is addressed in this review.

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“…Given the increasing apprehension among consumers regarding the presence of antibiotic residues in poultry products and the emergence of antibiotic-resistant strains, substitutes for antibiotics are necessary ( 28 , 29 ). Previous studies have confirmed that the active ingredients, derived or separated from hops, exhibit a substantial inhibitory effect on various pathogenic microorganisms [ Escherichia coli , S. aureus , Listeria monocytogenes ( 21 ), and C. perfringens ( 14 ), and they are considered safe and reliable ( 12 )].…”

Section: Discussionmentioning

confidence: 99%

Ex vivo pharmacokinetic/pharmacodynamic of hexahydrocolupulone against Clostridium perfringens in broiler chickens

Zhang,

Lu,

et al. 2024

Front. Vet. Sci.

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The economic impact of necrotizing enteritis (NE) resulting from Clostridium perfringens infection has been significant within the broiler industry. This study primarily investigated the antibacterial efficacy of hexahydrocolupulone against C. perfringens, and its pharmacokinetics within the ileal contents of broiler chickens. Additionally, a dosing regimen was developed based on the pharmacokinetic/pharmacodynamic (PK/PD) model specific to broiler chickens. Results of the study indicated that the minimum inhibitory concentration (MIC) of hexahydrocolupulone against C. perfringens ranged from 2 mg/L to 16 mg/L in MH broth. However, in ileal content, the MIC ranged from 8 mg/L to 64 mg/L. The mutation prevention concentration (MPC) in the culture medium was found to be 128 mg/L. After oral administration of hexahydrocolupulone at a single dosage of 10–40 mg/kg bodyweight, the peak concentration (Cmax), maximum concentration time (Tmax), and area under the concentration-time curve (AUC) in ileal content of broiler chickens were 291.42–3519.50 μg/g, 1–1.5 h, and 478.99–3121.41 μg h/g, respectively. By integrating the in vivo PK and ex vivo PD data, the AUC0-24h/MIC values required for achieving bacteriostatic, bactericidal, and bacterial eradication effects were determined to be 36.79, 52.67, and 62.71 h, respectively. A dosage regimen of 32.9 mg/kg at 24 h intervals for a duration of 3 days would yield therapeutic efficacy in broiler chickens against C. perfringens, provided that the MIC below 4 mg/L.

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Bacteriophage mediated control of necrotic enteritis caused by C. perfringens in broiler chickens.

Cited by 7 publications

References 35 publications

Antibiofilm activity of a lytic Salmonella phage on different Salmonella enterica serovars isolated from broiler farms

Antibiofilm activity of a lytic Salmonella phage on different Salmonella enterica serovars isolated from broiler farms

Necrotic Enteritis in Broiler Chickens: A Review on the Pathogen, Pathogenesis, and Prevention

Ex vivo pharmacokinetic/pharmacodynamic of hexahydrocolupulone against Clostridium perfringens in broiler chickens

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