Bacteriophage inhibition by extracts from phage-insensitive bacteria of the genus<i>Pseudomonas</i>

Mead, T. H.; Ende, M. Van Den

doi:10.1017/s0022172400015539

Cited by 5 publications

(9 citation statements)

References 22 publications

(4 reference statements)

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“…The selected strains were grown in oxygenated H.I.D. medium (Mead & van den Ende, 1953), at 37" for 15 hr in 700 ml. volumes in 1 1. bottles; tributyl phosphate was used as antifoam.…”

Section: Introductionmentioning

confidence: 99%

The Antigens of Pseudomonas aeruginosa Studied by the Ouchterlony Technique and Immuno-electrophoresis

Eeden¹

1967

Journal of General Microbiology

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SUMMARYPreliminary agglutination and precipitin tests to which 60 newly isolated strains of Pseudomonas aeruginosa were submitted confirmed the existence of at least five serological groups. Ouchterlony precipitin tests and immunoelectrophoresis experiments, made with the concentrated trichloroacetic acid extracts of eight strains representative of these groups, revealed the antigenic heterogeneity of this species while the presence of at least 12 antigens was demonstrated. One of these was common to seven of the eight strains. Each of five strains, representative of five different serological groups, possessed at least one antigen peculiar to itself. The remaining ones appeared to be distributed at random among the strains. At least three different antigens migrated towards the positive electrode, of which two appeared to be electrophoretically inhomogeneous.

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“…The selected strains were grown in oxygenated H.I.D. medium (Mead & van den Ende, 1953), at 37" for 15 hr in 700 ml. volumes in 1 1. bottles; tributyl phosphate was used as antifoam.…”

Section: Introductionmentioning

confidence: 99%

The Antigens of Pseudomonas aeruginosa Studied by the Ouchterlony Technique and Immuno-electrophoresis

Eeden¹

1967

Journal of General Microbiology

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“…at 37° C. in 20 1. of H.I.D. medium (Mead & van den Ende, 1953) which was gassed with oxygen, and were collected in a refrigerated Sharpies centrifuge. The supernatant fluid was neutralized with lactic acid, sterilized by nitration and used for a second run.…”

Section: Methodsmentioning

confidence: 99%

“…It was shown by Mead & van den Ende (1953) that an extract of a strain of Pseudomonas aeruginosa (Ps. LII) resistant to phages 5, 13 and 14 not only inhibited the formation of plaques by two of them (5 and 14) but also increased the plating efficiency of the third (13) when tested on a rough variant (Ps.…”

Section: Introductionmentioning

confidence: 99%

The Exhibition Phenomenon withPseudomonas AeruginosaPhage 13

Mead

1956

Epidemiol. Infect.

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1. The efficiency of plating of phage 13 on the semi-resistant strain Ps. LIII-3bi decreases as the initial bacterial population on the plate is diminished. Addition of Ps. LII extract to the phage before plating with low bacterial concentrations increases the plating efficiency (exhibition effect).2. An extract of a strain Ps. LIII-lb which is fully sensitive to phage 13 also causes exhibition of this phage on Ps. LIII-3bi.3. It is suggested that dissolved phage 13 receptors present in extracts of Ps. LII and Ps. LIII-lb have an affinity not only for the phage but also for suitable bacteria and thus cause exhibition. Inhibition (e.g. of phage 14 by the Ps. LII extract) is due to phage receptors having no tendency to adsorb to bacteria.4. The phage-exhibiting and inhibiting properties of a freeze-dried extract of Ps. LII active when dissolved in 0.85 % NaCl are lost if the solid is dissolved in water and the solution so obtained further diluted with 0.85 % NaCl. Activity is recovered slowly if NaCl is added to the aqueous solution, instantly if the solution is freeze-dried and the residue dissolved in 0.85 % NaCl.5. The inhibitory properties of the Ps. LII extract for phage 14 are more sensitive to heat and acidity or alkalinity than the exhibitory properties for phage 13.6. The absence of cross-precipitin reactions between Ps. LII and Ps. LUI-lb suggests that the phage 13 receptors of the two organisms are non-antigenic or different. I am grateful to Prof. M. van den Ende and my colleagues in this Unit for helpful discussions.

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“…Phages 68, PB1 (10), B3, D3, G101, and E79 have been described as cell wall-specific phages (19), but as yet no further studies have been undertaken to isolate the specific component of the wall to which these phages adsorb. The role of somatic receptors in phage adsorption was suggested from the work of Mead and van den Ende (27), who showed that Boivin and ethylene glycol extracts of P. aeruginosa L11 would irreversibly inactivate several of the phages tested. It is of interest to note that these extracts inactivated some phages for which strain L11 was not the host.…”

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confidence: 99%

Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO

Jarrell¹,

Kropinski²

1977

J Virol

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Bacteriophage inhibition by extracts from phage-insensitive bacteria of the genusPseudomonas

Cited by 5 publications

References 22 publications

The Antigens of Pseudomonas aeruginosa Studied by the Ouchterlony Technique and Immuno-electrophoresis

The Antigens of Pseudomonas aeruginosa Studied by the Ouchterlony Technique and Immuno-electrophoresis

The Exhibition Phenomenon withPseudomonas AeruginosaPhage 13

Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO

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