Bacteriophage-induced reduction in Salmonella Enteritidis counts in the crop of broiler chickens undergoing preslaughter feed withdrawal

Marietto-Gonçalves, Guilherme Augusto; Donato, Taís Cremasco; Baptista, Ana Angelita Sampaio; Corrêa, Isadora Mainieri de Oliveira; Garcia, K. C. O. D.; Filho, Raphael Lúcio Andreatti

doi:10.3382/ps.2013-03360

Cited by 19 publications

(8 citation statements)

References 17 publications

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“…To the best of our knowledge, this study presents the first results from the use of Salmonella phages at a large scale, in a poultry production system. Most of the previous studies were carried out under controlled conditions (Bardina et al, 2012; Gonçalves et al, 2013; Wong et al, 2014). Results obtained here are evidence of the fact that(a) there are no negative effects on the production parameters for the groups treated with the phage cocktail SalmoFREE ® ; and(b) a reduction in Salmonella incidence in the houses, which could be partially attributed to the phage treatment.…”

Section: Discussionmentioning

confidence: 99%

Phage cocktail SalmoFREE® reduces Salmonella on a commercial broiler farm

et al. 2019

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According to the World Health Organization, Salmonella is one of the most important zoonotic foodborne pathogens. Poultry products are thought to be the main source of Salmonella, which means that it is necessary to control Salmonella at the pre-harvest stage. Bacteriophages, acting as host-specific parasites of bacterial cells, represent one of the alternatives to antibiotics that can contribute to food safety and security. The present study evaluated the effectiveness of the bacteriophage cocktail SalmoFREE® to control Salmonella on a commercial broiler farm. We assessed the relationship between the use of SalmoFREE® and productivity parameters (feed conversion, weight gain, homogeneity). Two field trials (trial 1 n = 34,986; trial 2 n = 34,680) were carried out under commercial rearing conditions on a Colombian broiler farm with a record of Salmonella presence. Each trial comprised 2 control chicken houses and 2 experimental ones. SalmoFREE® and a control suspension were delivered in the drinking water at 3 time points in the production cycle, and the presence of Salmonella was assessed in cloacal swabs the day before and after the treatments. Results revealed that SalmoFREE® controls the incidence of Salmonella and does not affect the animals nor the production parameters, demonstrating its efficacy and innocuity at the production scale. We detected phage-specific genes in samples of total DNA extracted from ceca after the treatment with SalmoFREE®, and tested for the appearance of cocktail-resistant Salmonella, which showed to be an uncommon event. These results contribute relevant information to the adoption of phage therapy as an alternative to growth-promoter antibiotics on poultry farms.

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Section: Discussionmentioning

confidence: 99%

Phage cocktail SalmoFREE® reduces Salmonella on a commercial broiler farm

et al. 2019

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“…1 × 10 10 10/10 1.58 × 10 7 1 × 10 9 10/10 1 × 10 8 10/10 1 × 10 7 5/10 1 × 10 6 2/10 CZ14-1∆spiC∆rfaL 1 × 10 10 10/10 7.76 × 10 6 1 × 10 9 10/10 1 × 10 8 10/10 1 × 10 7 7/10 1 × 10 6 3/10 1 × 10 7 10/10 1 × 10 6 9/10 1 × 10 5 6/10 1 × 10 4 5/10 CZ14-1∆spiC∆nmpC 1 × 10 9 10/10 2.57 × 10 7 1 × 10 8 10/10 1 × 10 7 5/10 1 × 10 6 2/10 1 × 10 5 0/10 CZ14-1∆spiC∆rfaL 1 × 10 9 10/10 4.07 × 10 6 1 × 10 8 10/10 1 × 10 7 6/10 1 × 10 6 3/10 1 × 10 5 0/10…”

Section: Cz14-1∆spic∆nmpcmentioning

confidence: 99%

Evaluation of the Safety and Protection Efficacy of spiC and nmpC or rfaL Deletion Mutants of Salmonella Enteritidis as Live Vaccine Candidates for Poultry Non-Typhoidal Salmonellosis

Yue

Ren

et al. 2019

Vaccines

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Salmonella enterica serovar Enteritidis (S. Enteritidis) is a host-ranged pathogen that can infect both animals and humans. Poultry and poultry products are the main carriers of S. Enteritidis, which can be transmitted to humans through the food chain. To eradicate the prevalence of S. Enteritidis in poultry farms, it is necessary to develop novel vaccines against the pathogen. In this study, we constructed two vaccine candidates, CZ14-1∆spiC∆nmpC and CZ14-1∆spiC∆rfaL, and evaluated their protective efficacy. Both mutant strains were much less virulent than the parental strain, as determined by the 50% lethal dose (LD 50 ) for three-day-old specific-pathogen free (SPF) White Leghorns and Hyline White chickens. Immunization with the mutant candidates induced highly specific humoral immune responses and expression of cytokines IFN-γ, IL-1β, and IL-6. In addition, the mutant strains were found to be persistent for almost three weeks post-infection. The survival percentages of chickens immunized with CZ14-1∆spiC∆nmpC and CZ14-1∆spiC∆rfaL reached 80% and 75%, respectively, after challenge with the parental strain. Overall, these results demonstrate that the two mutant strains can be developed as live attenuated vaccines.

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“…The research to discovery new lytic bacteriophages able to improve the immunomagnetic separation assay needs be continuous because our test is capable to detect only the three serovars most prevalent, but there are more than 2,600 Salmonella serovars. A viable option is prepared a bacteriophages cocktail (Marietto Gonçalves et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse

et al. 2018

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Salmonella detection is a key point in food safety testing, because of the frequent association of this pathogen with food poisoning in humans. The standard bacteriological tests currently used for Salmonella-detection are time-consuming; therefore, there is a need to develop alternative methods to accelerate the detection. In order to accelerate Salmonella diagnosis, we used the immunomagnetic separation assay associated with bacteriophage P22 for the rapid detection of the following Salmonella serovars in chicken rinses of drumsticks, artificially contaminated with 5, 10, and 100 CFU/25mL of bacteria: Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg), Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium). The efficiency of the technique, represented by the time required for detection of positive and negative samples, was compared with that of the standard diagnostic tests used for this pathogen, the bacteriological assay and the polymerase chain reaction (PCR)-based test. This study confirmed the ability of the bacteriophage-associated immunomagnetic separation assay to identify 99.6% of Salmonella-positive samples of the three serovars tested. In contrast, the bacteriological assay and PCR-based test detected 95.1% and 98.5% of the Salmonella-positive samples respectively.

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Bacteriophage-induced reduction in Salmonella Enteritidis counts in the crop of broiler chickens undergoing preslaughter feed withdrawal

Cited by 19 publications

References 17 publications

Phage cocktail SalmoFREE® reduces Salmonella on a commercial broiler farm

Phage cocktail SalmoFREE® reduces Salmonella on a commercial broiler farm

Evaluation of the Safety and Protection Efficacy of spiC and nmpC or rfaL Deletion Mutants of Salmonella Enteritidis as Live Vaccine Candidates for Poultry Non-Typhoidal Salmonellosis

Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse

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