Bacteriophage Delivery Systems Based on Composite PolyHIPE/Nanocellulose Hydrogel Particles

Abstract

The role of bacteriophage therapy in medicine has recently regained an important place. Oral phage delivery for gastrointestinal treatment, transport through the stomach, and fast release in the duodenum is one of such applications. In this work, an efficient polyHIPE/hydrogel system for targeted delivery of bacteriophages with rapid release at the target site is presented. T7 bacteriophages were encapsulated in low crosslinked anionic nanocellulose-based hydrogels, which successfully protected phages at pH < 3.9 (stomach) and completely lost the hydrogel network at a pH above 3.9 (duodenum), allowing their release. Hydrogels with entrapped phages were crosslinked within highly porous spherical polyHIPE particles with an average diameter of 24 μm. PolyHIPE scaffold protects the hydrogels from mechanical stimuli during transport, preventing the collapse of the hydrogel structure and the unwanted phage release. On the other hand, small particle size, due to the large surface-to-volume ratio, enables rapid release at the target site. As a consequence, a fast zero-order release was achieved, providing improved patient compliance and reduced frequency of drug administration. The proposed system therefore exhibits significant potential for a targeted drug delivery in medicine and pharmacy.

Local epidermal growth factor delivery using nanopillared chitosan–gelatin films for melanogenesis and wound healing

“…28 The n value of the EGF-doped nano films was 1.73, while the value of the EGF-doped flat films was 1.66, indicating that anomalous transport occurred owing to Fickian diffusion and polymer relaxation as the films swelled. 29…”

“…28 The n value of the EGF-doped nano films was 1.73, while the value of the EGF-doped flat films was 1.66, indicating that anomalous transport occurred owing to Fickian diffusion and polymer relaxation as the films swelled. 29 Several reports discussed the toxicity of excessive EGF levels both for in vitro and in vivo studies. 27,30,31 In particular, the report by Ramineni and coworkers successfully demonstrated that a concentration regime between 1-5 ng mL −1 EGF provided maximal cell migration, cellular differentiation and wound healing.…”

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“…Further, breakdown in the DNA repair process facilitates the induction of colorectal cancer 21 Salmonella typhimurium 22 The bacterium produces AvrA protein that results in the proliferation of the IL-8 intestinal epithelial cell lining, thereby facilitating the induction of colorectal cancer 22 The cease in proliferation in the growth of the gut microbiome resulting in dysbiosis is carried out by certain bacteriophages specific to the gut microbiome. 23 Phage M13 23 Bacteroides fragilis (Enterotoxigenic) 24 Phage VA7 24 Escherichia coli 15,16,25 Phage T7 25 , Phage ZCEC5 16 , Phage T4…”

CuO Nanoparticle@Polystyrene Hierarchical Porous Foam for the Effective Encapsulation of Octadecanol as a Phase Changing Thermal Energy Storage Material

“…28−32 To date, porous clay, 33 mineralbased materials, 14 metallic foams, 34 porous carbon, 35 and porous polymers 36,37 are the most commonly used framework materials for shape-stabilized PCM. High internal phase emulsion templated polymers (PHP) as porous polymers have been used in a wide variety of applications such as adsorption, 38,39 purification, 40,41 separation, 42 controlled release, 43,44 and scaffolding, 45 and there is limited research in energy storage application. 46,47 Recently, porous PHP foams have begun to gain attention for the stabilization of PCMs owing to their superior features such as shape stabilization, enclosure ability, high loading rate, excellent structural stability, and interpenetrating pore morphology.…”

“…To overcome these limitations, in addition to PCM microcapsules, − the application of shape-stable porous materials as a PCM carrier has received great interest in the literature. − To date, porous clay, mineral-based materials, metallic foams, porous carbon, and porous polymers , are the most commonly used framework materials for shape-stabilized PCM. High internal phase emulsion templated polymers (PHP) as porous polymers have been used in a wide variety of applications such as adsorption, , purification, , separation, controlled release, , and scaffolding, and there is limited research in energy storage application. , Recently, porous PHP foams have begun to gain attention for the stabilization of PCMs owing to their superior features such as shape stabilization, enclosure ability, high loading rate, excellent structural stability, and interpenetrating pore morphology. − PHPs are monolithic, controllable, highly porous polymers with low density, high specific surface area, highly cross-linked polymer matrix, the porosity of which is created by the continuous phase of emulsions. PHPs in particular stand out for their large gaps bounded by small windows on the skeleton.…”