Bacteriophage amplification – A comparison of selected methods

Skaradzińska, Aneta; Ochocka, Marta; Śliwka, Paulina; Kuźmińska-Bajor, Marta; Skaradziński, Grzegorz; Friese, Anika; Roschanski, Nicole; Murugaiyan, Jayaseelan; Roesler, Uwe

doi:10.1016/j.jviromet.2020.113856

Cited by 11 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S3 ). This discovery will be significant for guiding the preparation of high-titer phage vB_Pae_QDWS because there is no optimal, universal method for phage amplification ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

Quorum Sensing Promotes Phage Infection in Pseudomonas aeruginosa PAO1

Xuan

Lin

Tan

et al. 2022

mBio

View full text Add to dashboard Cite

Phage resistance is a major limitation of phage therapy, and understanding the mechanisms by which bacteria block phage infection is critical for the successful application of phage therapy. In the present study, we found that Pseudomonas aeruginosa PAO1 uses las QS to promote phage infection by upregulating the expression of galU , which is necessary for the synthesis of phage receptor lipopolysaccharides.

show abstract

“…S3 ). This discovery will be significant for guiding the preparation of high-titer phage vB_Pae_QDWS because there is no optimal, universal method for phage amplification ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

Quorum Sensing Promotes Phage Infection in Pseudomonas aeruginosa PAO1

Xuan

Lin

Tan

et al. 2022

mBio

View full text Add to dashboard Cite

show abstract

“…The amplification of bacteriophages in liquid culture was based on Skaradzińska’s method ( Skaradzińska et al, 2020 ) and the host strain of the bacteriophage GRNsp6 was ATCC13076. The phages were concentrated and purified using PEG8000 and cesium chloride (CsCl) density gradient ultracentrifugation according to the methods ( Bourdin et al, 2014 ; Luong et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Salmonella Phages Affect the Intestinal Barrier in Chicks by Altering the Composition of Early Intestinal Flora: Association With Time of Phage Use

Zhao

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Phages show promise in replacing antibiotics to treat or prevent bacterial diseases in the chicken breeding industry. Chicks are easily affected by their environment during early growth. Thus, this study investigated whether oral phages could affect the intestinal barrier function of chicks with a focus on the cecal microbiome. In a two-week trial, forty one-day-old hens were randomly divided into four groups: (1) NC, negative control; (2) Phage 1, 109 PFU phage/day (days 3–5); (3) Phage 2, 109 PFU phage/day (days 8–10); and (4) AMX, 1 mg/mL amoxicillin/day (days 8–10). High-throughput sequencing results of cecal contents showed that oral administration of phages significantly affected microbial community structure and community composition, and increased the relative abundance of Enterococcus. The number of different species in the Phage 1 group was much higher than that in the Phage 2 group, and differences in alpha and beta diversity also indicated that the magnitude of changes in the composition of the cecal microbiota correlated with the time of phage use. Particularly in the first stage of cecal microbiota development, oral administration of bacteriophages targeting Salmonella may cause substantial changes in chicks, as evidenced by the results of the PICRUSt2 software function prediction, reminding us to be cautious about the time of phage use in chicks and to avoid high oral doses of phages during the first stage. Additionally, the Phage 2 samples not only showed a significant increase in the relative abundance of Bifidobacterium and Subdoligranulum, but also improved the intestinal morphology (jejunum) and increased the mRNA expression level of occludin and ZO-1. We concluded that phages do not directly interact with eukaryotic cells. The enhancement of intestinal barrier function by phages in chicks may be related to changes in the intestinal flora induced by phages. This implies that phages may affect intestinal health by regulating the intestinal flora. This study provides new ideas for phage prevention of intestinal bacterial infections and promotes large-scale application of phages in the poultry industry.

show abstract

“…Then the culture was centrifuged and filtered to remove the remaining bacterial cells to obtain phage suspension (1 × 10 8 PFU mL −1 ). [ 33 ]…”

Section: Methodsmentioning

confidence: 99%

“…Then the culture was centrifuged and filtered to remove the remaining bacterial cells to obtain phage suspension (1 × 10 8 PFU mL −1 ). [33] Antivirus Assays: In the evaluation of photo-virucidal properties of samples, The PPE samples and BBR@ PPE samples were cut with a size of 2 × 2 cm 2 and spotted with 10 µL T7 phage dilution. All samples were put into the ultraviolet cross linker NAI5000 and exposed under the light or dark condition for 60 min.…”

Section: Methodsmentioning

confidence: 99%

Visible‐Light‐Driven and Self‐Hydrogen‐Donated Nanofibers Enable Rapid‐Deployable Antimicrobial Bioprotection

Chang

et al. 2021

Small

View full text Add to dashboard Cite

The novel coronavirus SARS‐CoV‐2 has prompted a worldwide pandemic and poses a great threat to public safety and global economies. Most present personal protective equipment (PPE) used to intercept pathogenic microorganisms is deficient in biocidal properties. Herein, we present green nanofibers with effective antibacterial and antiviral activities that can provide sustainable bioprotection by continuously producing reactive oxygen species (ROS). The superiority of the design is that the nanofibers can absorb and store visible light energy and maintain the activity under light or dark environment. Moreover, the nanofibers can uninterruptedly release ROS in the absence of an external hydrogen donor, acting as a biocide under all weather conditions. A facile spraying method is proposed to rapidly deploy the functional nanofibers to existing PPE, such as protective suits and masks. The modified PPE exhibit stable ROS production, excellent capacity for storing activity potential, long‐term durability, and high bactericidal (>99.9%) and viricidal (>99.999%) efficacies.

show abstract

Bacteriophage amplification – A comparison of selected methods

Cited by 11 publications

References 36 publications

Quorum Sensing Promotes Phage Infection in Pseudomonas aeruginosa PAO1

Quorum Sensing Promotes Phage Infection in Pseudomonas aeruginosa PAO1

Salmonella Phages Affect the Intestinal Barrier in Chicks by Altering the Composition of Early Intestinal Flora: Association With Time of Phage Use

Visible‐Light‐Driven and Self‐Hydrogen‐Donated Nanofibers Enable Rapid‐Deployable Antimicrobial Bioprotection

Contact Info

Product

Resources

About