Bacteriolytic Activity study of Aerophage on Pseudomonas aeruginosa

Abstract

Background:The increasing incidence of antibiotic resistance in bacterial pathogens has justified a reassessment of the value of phages as antibacterial agents for medical applications. Objective: To assess the effect of bacteriolytic activity against P. aeruginosa by use multi methods for isolation of bacteriophage. Patients and Methods: This study was performed from from November, 2016 till April, 2017. A total of 60 samples collected from urine, stool, diabetes leg, ear, burns and wounds infections in General Baqubah Hospital to isolate Pseudomonas aeruginosa and tested for 6 groups of antibiotics. Aerophage was isolated using three methods: spotting; double -layer agar plate and liquid broth method. After enrichment of aerophage, the bacteriolytic activity of aerophage was done by spotting method. Results: Pseudomonas aeruginosa isolates resistance were 10(43.5%) for Aztreonem and 8(34.8 %) to Ticarcillin-Claculanic acid followed by Imipenem 4(17.4%), Gentamicin and when the study done Levofloxacin 3(13%) for each finally Cefepime2(8.7%). high significant differences of aztreonem with both of cefepim and gentamycin (P<0.01) while significant differences of aztreonem with both imipenim and levofloxacillin (P<0.05%). while the results of bacteriolytic activity showed all ear and leg diabetes isolates were sensitive to aerophage (100%) followed by urine isolates(75%), stool isolates(50%) and burns isolates(25%) while all wound isolates were resist to aerophage(100%). The result of relation between Antibiotic resistance phenotype profile and Aerophage sensitivity that no detect to susceptibility of isolates to aerophage but the source of isolates may be detected to infection with aerophage. Conclusion:Aztreonem antibiotic was widely used in Baqubah city recently. All three methods to isolate aerophage were effective. The result of relation between Antibiotic resistance phenotype profile and Aerophage sensitivity that no detect to susceptibility of isolates to aerophage but the source of isolates may be detected to infection with aerophage.