Bacteriocins of Some Groups of Gram-Negative Bacteria

Balko, O.I.; Balko, O.B.; Avdeeva, L.V.

doi:10.15407/microbiolj82.03.071

Cited by 3 publications

(4 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that pyocins are characterized by diff erent mechanisms of infl uence on sensitive cultures. Th us, pyocin S1, S2, S3, and AP41 possess DNase activity, pyocin S4 has tRNase properties, and pyocin S5 inhibits microorganisms due to the ability to form pores (Soltani et al, 2021;Michel-Briand & Baysse, 2002;Riley & Chavan, 2007;Balko, 2012). According to our results, bacteriocin genes with DNase (pyocin S1, S2, and S3) and tRNase activity (pyocin S4) were detected with the same frequency in all groups of producers, while bacteriocin genes with poreforming activity (pyocin S5) were observed most oft en among producers of maximally and highly active bacteriocins.…”

Section: Materials Andmentioning

confidence: 99%

Phenotypic and Genotypic Criteria for the Screening of Highly Active S-Type Pyocins Pseudomonas aeruginosa Producers

Balko,

Zelena,

Balko

et al. 2024

Mikrobiol. Z.

View full text Add to dashboard Cite

Bacteriocins of Pseudomonas aeruginosa, especially S-type pyocins, show high efficiency as analogs of antimicrobial drugs. Various screening methods can be used to identify producers of highly active pyocins, but there are no clear criteria for selecting perspective strains. The aim of this work was to determine criteria that can be used during phenotypic and genotypic screening for the selection of perspective highly active S-type pyocin P. aeruginosa producers. Methods. The objects of investigation were 40 P. aeruginosa strains. Pyocins were obtained from each culture, relative coefficients of activity spectrum and sensitivity were determined for all the strains used. The obtained results of the phenotypic screening were compared with the data of the genotypic screening. Results. The use of the proposed method of activity assessment according to the lysis intensity made it possible to phenotypically assess the expression of pyocin genes. It was established that according to the new criteria, only one strain — P. aeruginosa UCM B-333 — can be included in the group of the most active pyocin producers that inhibit the growth of more than 75% of indicator cultures. The majority of representatives of maximally and highly active producers were characterized by high resistance to the action of other pyocins, which can be considered as an additional criterion for the selection of perspective strains. During genotypic screening, it was established that the quantity of pyocin genes in the genome cannot be interpreted as a clear criterion of the producer’s perspective. However, 50% of representatives of maximally and highly active pyocin producers were characterized by the presence of two pyocin genes, while in 47.7% of moderately active and 54.5% of low active producers, one pyocin gene was detected more often. It was established that with widening the bacteriocin activity spectrum, the detection frequency of pyocin S1 and S5 genes increases, and for pyocin S2 and S3 genes — decreases. Thus, among the producers of maximally and highly active bacteriocins, pyocin S1 and S5 genes were identified with the highest frequency — 42.8% and 78.6%, and pyocin S2 and S3 genes — with the lowest one — 28.6% and 7.1%, respectively. Gene of pyocin S4 with tRNase activity were detected with equally high frequency in all groups of producers. Conclusions. The method of activity assessment by the lysis intensity allows not only to determine the presence of pyocins, but also to phenotypically evaluate the level of their expression, which is an important criterion for the selection of perspective producers. Bacteriocins with a wider activity spectrum are synthesized by P. aeruginosa strains with higher resistance to the action of pyocins from other cultures. The most optimal genotypic criterion for the selection of a highly perspective pyocin producer, detection of genes combination of bacteriocins with different mechanisms of action — with DNase activity (pyocin S1) and the ability to pore formation (pyocin S5) — can be considered.

show abstract

Section: Materials Andmentioning

confidence: 99%

Phenotypic and Genotypic Criteria for the Screening of Highly Active S-Type Pyocins Pseudomonas aeruginosa Producers

Balko,

Zelena,

Balko

et al. 2024

Mikrobiol. Z.

View full text Add to dashboard Cite

show abstract

“…Bacteriocins are antimicrobial substances synthesized by majority of microorganisms that are able to inhibit the growth of closely-related strains (Somsap et al, 2015;Kongkum et al, 2016). Some researchers consider them to be promising, alternative means to available preparation for influence on plant diseases agents (Rooney et al, 2020;Balko et al, 2020). These substances possess high antibacterial activity, not lower than in classic antibiotics, narrow action spectrum, which limits their impact to agents of particular disease only, do not pollute the environment and are safety for human (Ghequire and De Mot, 2014;Soltani et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…The production of three type's bacteriocins: pyocins of R, F and S-type is described for Pseudomonas aeruginosa (Ghequire and De Mot, 2014;Behrens et al, 2017). The first two types are high molecular weight structures, analogues of phage-tails with a molecular weight of 1-10 МDa (Balko, 2012;Scholl, 2017). Colicin-like S-type pyocins belong to proteins with a molecular weight of 40-70 кDа (Behrens et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Pyocin S5 Activity Against Pseudomonas Syringae Phytopathogenic Strains

Balko,

Zelena,

Balko

et al. 2024

J microb biotech food sci

View full text Add to dashboard Cite

Pseudomonas syringae is the most frequently isolated bacterial plant pathogens. Previously we revealed a number of Pseudomonas aeruginosa strains – producers of bacteriocins (pyocins) with high activity against P. syringae. However, it was unknown, which type of pyocins possesses the activity against P. syringae strains. S5 and S1 pyocin genes were observed in the genome of most active strain–producer P. aeruginosa UCM В-333. Pyocins were induced by nalidixic acid (100 μg/ml). Using DEAE-cellulose chromatography we isolated two bacteriocin groups from initial lysate. Substances from the 10th fraction had nuclease activity (pyocin S1) and the 27th fraction - pore-forming activity (pyocin S5). It was revealed that S1 pyocin didn’t lead to growth inhibition of studied bacterial plant pathogens. However pyocin S5 caused appearance of growth inhibition area with diameter 14-20 mm. So, pyocins of S1 and S5 types are active substances in P. aeruginosa UCM В-333 lysates, but only S5 pyocin possess activity against P. syringae strains. fpt A receptor gene was revealed in genomes of all analyzed cultures, including used phytopathogenic strains. This fact can explain wide spectrum of pyocin S5 activity. It is suggested that pyocin S5 can be a potential biocontrol agent against phytopathogenic P. syringae strains.

show abstract

“…Colicin-like S-type pyocins are characterized by molecular weight of 30-100 kDa and cause microorganism death by different mechanisms [5,6]. Properties of these substances, peculiarities of their induction and killer action so as ability of some strains to produce them are described [7,8]. Influence of induction conditions can lead to simultaneous synthesis of both types of bacteriocins by producer cultures [9,10].…”

mentioning

confidence: 99%

Interaction between S-Type Pyocins and Microcin-II-Like Bacteriocins in Pseudomonas aeruginosa

Balko¹

2021

Mikrobiol. Z.

Self Cite

View full text Add to dashboard Cite

According to our previous results, S-type bacteriocins of Pseudomonas aeruginosa are characterized by high activity against phytopathogenic Pseudomonas syringae strains. In addition to these pyocins producing strains are able to synthesize microcin-II-like bacteriocins. Presence of interaction between these two killer factors can determine methods of their use and activity increase of bacteriocins with antiphytopathogenic properties. The aim of the work was to test possibility of interaction between S-type pyocins and microcin-II-like bacteriocins of P. aeruginosa. Methods. The objects of the study were pyocins produced by 6 P. aeruginosa strains. Killer factors in composition of induced lysates were concentrated by 70% ammonium sulphate precipitation, dialyzed through dialysis membrane with molecular weight cut-off (MWCO) 3.5 kDa. Then ion-exchange chromatography with DEAE-cellulose, gel filtration with Sephadex G-75 and ultracentrifugation at 215.000 g for 1 and 4 hours were used for their separation. Protein concentration and antimicrobial activity were determined in obtained fractions. Visualization of proteins in active fraction composition was conducted by electrophoresis according to the Laemmli method. Results. Under ion-exchange chromatography with DEAE-cellulose application elution of bacteriocins available in lysate composition occurs simultaneously. The highest indices of activity and protein concentration were in the 4th fraction, containing two protein bands with molecular weight near 58 and 9 kDa, which are typical for S5 pyocin and microcin-II-like bacteriocins of P. aeruginosa. Further gel filtration of sampled fractions through Sephadex G-75 allowed to separate noted killer factors and obtaine purified fraction containing microcin-II-like pyocins only. Application of ultracentrifugation during 1 hour didn’t precipitate studied bacteriocins, whereas during 4 hours – lead to their separation. At the same time a twofold increase of activity indices for S-type pyocins in precipitates and for microcin-IIlike killer factors – in supernatants were observed. However achieved concentration was characterized by short-term effect, since in 14 days activity of supernatants decreased by 4–16 times, and for precipitates – by 80–640 times. Then revealed tendency for activity decrease continued. Conclusions. S-type pyocins and microcin-II-like bacteriocins of P. aeruginosa interact with each other, that ensures their stabilization and protects again destruction. Application of methods that cause separation of these killer factors is inexpedient, since it results into considerable decrease of bacteriocin activity indices.

show abstract

Bacteriocins of Some Groups of Gram-Negative Bacteria

Cited by 3 publications

References 94 publications

Phenotypic and Genotypic Criteria for the Screening of Highly Active S-Type Pyocins Pseudomonas aeruginosa Producers

Phenotypic and Genotypic Criteria for the Screening of Highly Active S-Type Pyocins Pseudomonas aeruginosa Producers

Pyocin S5 Activity Against Pseudomonas Syringae Phytopathogenic Strains

Interaction between S-Type Pyocins and Microcin-II-Like Bacteriocins in Pseudomonas aeruginosa

Contact Info

Product

Resources

About