Bacteriocins of gram-positive bacteria.

Tagg, John; Dajani, Adnan S.; Wannamaker, Lewis W.

doi:10.1128/mmbr.40.3.722-756.1976

Cited by 960 publications

(567 citation statements)

References 171 publications

Supporting

Mentioning

537

Contrasting

Unclassified

Order By: Relevance

“…However, the present study indicates that three strains of C. maltaromaticum, three strains of C. divergens and two strains which showed high similarity to an uncultured bacterium clone wet197 inhibited growth of the ¢sh pathogen C. maltaromaticum CCUG 34645 originally isolated from cutthroat trout (Salmo clarki) (Hiu, Holt, Siriranganathan, Seidler & Fryer 1984). Inhibition of C. maltaromaticum CCUG 34645 by three carnobacteria strains observed in the present study is not unusual, as some bacteriocins produced by LAB inhibit closely related species (Tagg, Dajani & Wannamaker 1976).…”

Section: Discussionmentioning

confidence: 38%

The ability of carnobacteria isolated from fish intestine to inhibit growth of fish pathogenic bacteria: a screening study

Ringø

2008

Aquaculture Research

View full text Add to dashboard Cite

In the present study a microtitre plate assay was used to evaluate antagonistic activity of 157 intestinal bacteria belonging to Carnobacterium isolated from Arctic charr (Salvelinus alpinus L.), Atlantic salmon (Salmo salar L.) and wolf fish (Anarhichas lupus L.) against fish pathogenic bacteria. One hundred and forty‐nine strains isolated from Arctic charr fed; (a) different lipid levels and (b) different fatty acids were screened for their ability to inhibit growth of the fish pathogen Aeromonas salmonicida ssp. salmonicida strain AL 2020 (the causative agent of furunculosis). Carnobacterium maltaromaticum and Carnobacterium mobile isolated from fish fed a low‐lipid diet inhibited growth of the pathogen, while none of the Carnobacterium divergens isolated from fish fed the high‐lipid diet had this ability. When Arctic charr was fed different fatty acids, was the frequency of antibacterial ability of C. maltaromaticum highest in strains isolated from fish fed 4%α‐linolenic acid (18:3 n‐3) and lowest in strains isolated from fish fed 4% linoleic acid (18:2 n‐6). Extracellular growth inhibitory compounds harvested in exponential and stationary growth phase from eight carnobacteria strains isolated from three fish species were tested for their ability to inhibit growth of six fish pathogens [A. salmonicida, Vibrio splendidus strain VS11, Vibrio salmonicida strain LFI 315, Vibrio anguillarum strain LFI 317, Moritella (Vibrio) viscosa strain LFI 5000 and C. maltaromaticum (piscicola) CCUG 34645]. The highest antibacterial activity was found when cellular extracts of the producer isolate were harvested in stationary growth phase. Scanning electron microscopy (SEM) investigations of A. salmonicida showed that cell morphology was affected by the inhibitory substance produce by strain 8M851, a Carnobacterium inhibens‐like bacteria.

show abstract

Section: Discussionmentioning

confidence: 38%

The ability of carnobacteria isolated from fish intestine to inhibit growth of fish pathogenic bacteria: a screening study

Ringø

2008

Aquaculture Research

View full text Add to dashboard Cite

show abstract

“…Likewise reported by Anupad and Na-Bangchng (2007), where the Pumilicin 4 isolated from B. pumilus WAPB4 showed activity with the pH ranging from 3.0 to 9.0 with optimum activity at alkaline pH values. Lee and Kim (2011) indicated antimicrobial activity of B. subtilis between pH ranges from 4.0 to 10.0, while Karmen and Matijasic (2003) reported confirmed that the antimicrobial agent produced by the tested strain of B. pumilus was similar according to the criteria outlined by Tagg, Dajani, and Wannamaker (1976). The complete inactivation of the antimicrobial substance after the treatment with pepsin and K-proteinase was observed, and only 6% activity was observed after trypsin treatment.…”

Section: Strainsmentioning

confidence: 89%

Antimicrobial activity of partially characterized analytes fromBacillus pumilus(B2)

et al. 2017

View full text Add to dashboard Cite

This study was carried out to characterize the antimicrobial substance produced by the strain of Bacillus pumilus (B2) obtained from Novozymes Biologicals Inc. to compare its antimicrobial activity by agar well diffusion assay and bacteriocin activity assay via critical dilution method against seven different strains of Vibrio spp., specifically V. alginolyticus (A01), V. cholerae (C01), V. fluvialis (F01, F02), V herveyii (H), V. mimicus (M01), V. parahaemolyticus (P01) and V. vulnificus (V01, V02). All Vibrio spp. were isolated from the hemolymph and intestine of the white faeces disease‐infected moribund pacific white‐leg shrimp Litopenaeus vannamei (Boone 1931) and one strain (V. harveyi) from its diseased postlarva. The cell‐free neutralized supernatant (CFNS) of B2 showed moderate thermo‐stability being stable up to 70°C for 60 min with, however, reducing activity above 80°C for 20 min. B2 antimicrobials showed a stable activity within the pH ranging from 6 to 10 at room temperature and at 4°C, while residual antimicrobial activity of crude CFNS showed tolerance to salinity up to 7% of sodium chloride below 4°C. No B2 activity was obtained while exposed to proteolytic enzyme, such as proteinase k and pepsin, while its activity kept stable exposed to lipase. Initial B2 characterization for antimicrobial substance in CFNS revealed proteinaceous in nature owing to activity loss against proteolytic enzymes and no lipid moiety activity against lipase, which could be categorized as bacteriocin‐like inhibitory substance having potential application against several strains of Vibrio spp. in aquaculture.

show abstract

“…A number of reference strains were sourced from the Microbiology Strain Collection, University of Otago. These included nine-indicator isolates (I 1 -I 9 ) typically used for bacteriocin production-typing (P-typing) (Tagg et al, 1976), Streptococcus salivarius strain 20P3 (producer of salivaricin A), Streptococcus pyogenes strain T11 (producer of salivaricin A1), Staphylococcus simulans strain SI10, S. aureus strain SI11, S. aureus strain C55 (producer of staphylococcin C55), S. epidermidis strain 5 (producer of Pep5), Staphylococcus warneri strain ATTC27836 (producer of warnericin), S. epidermidis strain 163 (producer of epidermin) and S. epidermidis strain K7 (producer of epilancin).…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

Identification of a haemolysin-like peptide with antibacterial activity using the draft genome sequence ofStaphylococcus epidermidisstrain A487

Mahrous¹,

Jack²,

Sandiford³

et al. 2011

FEMS Immunol Med Microbiol

View full text Add to dashboard Cite

Our interest in Staphylococcus epidermidis strain A487 was prompted by the unusual nature of its inhibitory activity in screening tests against methicillin-resistant Staphylococcus aureus isolates. The inhibitory activity was detected in deferred antagonism tests only if the agar plate was preheated for at least 35 min at ≥ 55 °C before inoculation of the indicator bacteria, this phenomenon indicating possible involvement of a heat-labile immunity agent or protease. The inhibitor was purified to homogeneity by ammonium sulphate precipitation, followed by cation-exchange and reversed-phase chromatography. Tandem MS revealed a novel peptide of molecular weight 2588.4 Da. The draft genome sequence of strain A487 was determined using 454 GS FLX technology, allowing the identification of the structural gene (hlp) encoding the mature peptide MQFITDLIKKAVDFFKGLFGNK. The deduced amino acid sequence of peptide 487 exhibited 70.8% similarity to that of a putative haemolysin from Staphylococcus cohnii. Analysis of the genome of strain A487 showed several additional inhibitor-encoding genes, including hld, the determinant for staphylococcal δ-lysin. This work indicates that potentially useful inhibitors could be overlooked in agar-based inhibitor screening programmes lacking a heat pretreatment step and also highlights the utility of draft genome sequence examination in antibacterial agent discovery.

show abstract

Bacteriocins of gram-positive bacteria.

Cited by 960 publications

References 171 publications

The ability of carnobacteria isolated from fish intestine to inhibit growth of fish pathogenic bacteria: a screening study

The ability of carnobacteria isolated from fish intestine to inhibit growth of fish pathogenic bacteria: a screening study

Antimicrobial activity of partially characterized analytes fromBacillus pumilus(B2)

Identification of a haemolysin-like peptide with antibacterial activity using the draft genome sequence ofStaphylococcus epidermidisstrain A487

Contact Info

Product

Resources

About