Bacteriocin ST91KM, produced by Streptococcus gallolyticus subsp. macedonicus ST91KM, is a narrow-spectrum peptide active against bacteria associated with mastitis in dairy cattle

Abstract

Streptococcus gallolyticus subsp. macedonicus ST91KM produces a bacteriocin (macedocin ST91KM) active against Streptococcus agalactiae, Streptococcus dysgalactiae subsp. dysgalactiae, Streptococcus uberis, Staphylococcus aureus, and Staphylococcus epidermidis. Macedocin ST91KM is, according to tricine-SDS PAGE, between 2.0 and 2.5 kDa in size. Antimicrobial activity remained unchanged after 2 h of incubation at pH 2.0-10.0 and after 100 min at 100 degrees C. The peptide was inactivated after 20 min at 121 degrees C and when treated with proteolytic enzymes. Treatment with alpha-amylase had no effect on activity, suggesting that the mode of action does not depend on glycosylation. Amplification of the genome of strain ST91KM with primers designed from the macedocin precursor gene (mcdA) produced 2 fragments (approximately 375 and 220 bp) instead of one 150-bp fragment, as recorded for macedocin produced by Streptococcus gallolyticus subsp. macedonicus ACA-DC 198. Strain ACA-DC 198 was not available. However, DNA amplified from strain LMG 18488 (ACA-DC 206), genetically closely related to strain ACA-DC 198, revealed 99% homology to the mcdA of strain ACA-DC 198 (accession No. DQ835394). Macedocin ST91KM may thus be a second putative bacteriocin described for Streptococcus gallolyticus subsp. macedonicus.

Mode of action and in vitro susceptibility of mastitis pathogens to macedocin ST91KM and preparation of a teat seal containing the bacteriocin

“…macedonicus ST91KM, isolated from Bulgarian goat yoghurt, produces the bacteriocin macedocin ST91KM (26). The peptide has a narrow spectrum of antibacterial activity and inhibits the growth of As far as we could determine, only one other bacteriocin has been described for S. macedonicus, i.e.…”

KARAKTERISASI BAKTERIOSIN PADA BAKTERI ASAM LAKTAT Lactobacillus paracasei DARI VIRGIN COCONUT OIL

“…(Sankar et al, 2012), (Todorov et al, 2011), (Sankar et al, 2012), (Udhayashree et al, 2012 mengukur berat molekul. Pada umumnya peneliti-peneliti yang lain juga memvariasikan ammonium sulfatnya dengan variasi 20 %, 40 %, 60 % dan 80%, seperti yang dilakukan oleh Pieterse, (Pieterse, Todorov and Dicks, 2008) yang mempelajari pemurnian parsial bakteriosin ST91KM yang dihasilkan oleh Streptococcus gallotycus subsp.macedonicus ST91KM dengan pengendapan ammonium sulfat juga dan dengan variasi ammonium sulfat juga 20%, 40%, 60%, dan 80%. Tidak berbeda jauh dengan, (Gautam and Sharma, 2009), yang mengisolasi bakteriosin dari Lactobacillus plantarum dari udang laut memvariasikan ammonium sulfat nya dengan variasi 10, 20, 30, 40, 50 dan 60 % dan mendapat kan hasil yang baik pada pengendapan 50 %.…”

Bacterial Antagonist Mediated Protein Molecules

“…macedonicus could also be considered an alternative in treatment of mastitis coil since this peptide inhibits Streptococcus agalactiae, Streptococcus dysgalactiae subsp. dysgalactiae, Streptococcus uberis, Staphylococcus aureus and Staphylococcus epidermidis, including resistant strains methicillin and oxacillin [158][159]. Other peptide named AP-CECT7121 has also shown in vitro efficacy against Staphylococcus aureus, Streptococcus dysgalactiae, S. uberis, S. agalactiae strains isolated from dairy cattle with mastitis [81].…”