Mode of action and in vitro susceptibility of mastitis pathogens to macedocin ST91KM and preparation of a teat seal containing the bacteriocin

Abstract

Mastitis is considered to be the most economically costly disease affecting the dairy industry. Regular as it is effective against pathogens that display resistance to conventional antibiotic therapy.

In vitro ability of lactic acid bacteria to inhibit mastitis-causing pathogens

“…In order to reduce the usage of intramammary antibiotic formulations, sustainable therapeutics are urgently needed (Barkema et al, 2006;McEwen and Fedorka-Cray, 2002). Probiotic products against udder diseases have been developed during the last decades and comprise bacteriocins for external application, such as lacticin 3147 or nisin as teat dips (Klostermann et al, 2010;Sears et al, 1992a), intramammarily applicated bacteriocins (Cao et al, 2007;Meaney et al, 2001;Pieterse et al, 2010;Ryan et al, 1998;Sears et al, 1992b;Twomey et al, 2000) and live probiotic microorganisms Frola et al, 2012). Some authors indicate that the probiotic strain should be obtained from the same ecological niche where the end product will be potentially applied, because autochthonous strains are better adapted to the specific environment and are more capable of epithelial colonization (Saarela et al, 2000).…”

Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

“…Currently, the application of bacteriocins in the prevention of mastitis is available through products such as Wipe Out â , using nisin produced by certain strains of Lactococcus lactis in disinfection cloths (Wu et al 2007), and teat seals, which are oil-based formulations that can be used to cover the teat canal at the end of the lactation and contain an antistaphylococcal bacteriocin such as lacticin 3147 produced by Lact. lactis DPC 3147 (Ryan et al 1998) or macedocin ST91KM produced by Streptococcus macedonicus ST91KM (Pieterse et al 2010). Although bacteriocins produced by staphylococci display a promising antibacterial spectrum, which includes the inhibition of antibiotic-resistant strains of staphylococci, their use in mastitis treatment is still limited (Nascimento et al 2006;Coelho et al 2007;Kim et al 2010).…”

Activity of essential oils from spices against Staphylococcus spp. isolated from bovine mastitis

“…Alternative treatments to bovine mastitis with bacteriocins (Pieterse et al, 2010) and plant derived compounds (Baskaran et al, 2009;Mubarack et al, 2011) have been described. Essential oils (EO) are classified as GRAS (generally regarded as safe), show antibacterial proprieties and resistance has not been reported after prolonged exposure.…”