Bacteriocin-nanoconjugates as emerging compounds for enhancing antimicrobial activity of bacteriocins

Sidhu, Parveen Kaur; Nehra, Kiran

doi:10.1016/j.jksus.2017.12.007

Cited by 34 publications

(34 citation statements)

References 60 publications

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“…LAB bacteriocins are extensively used, because they have been accorded GRAS status by the U.S. FDA. Nisin and pediocin are well-known bacteriocins produced by gram-positive bacteria [ 16 ] and are already approved for commercial use by the FDA. Nisin, which is synthesized as a precursor peptide produced by Lactococcus lactis , contains 57 amino acids and has a molecular weight (MW) of 3354 daltons.…”

Section: Different Bacteriocins and Their Spectrummentioning

confidence: 99%

Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins

Naskar

Kim

2021

Pharmaceutics

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Bacteriocins are antimicrobial peptides or proteinaceous materials produced by bacteria against pathogens. These molecules have high efficiency and specificity and are equipped with many properties useful in food-related applications, such as food preservatives and additives, as well as biomedical applications, such as serving as alternatives to current antibacterial, antiviral, anticancer, and antibiofilm agents. Despite their advantages as alternative therapeutics over existing strategies, several limitations of bacteriocins, such as the high cost of isolation and purification, narrow spectrum of activity, low stability and solubility, and easy enzymatic degradation, need to be improved. Nanomaterials are promising agents in many biological applications. They are widely used in the conjugation or decoration of bacteriocins to augment the activity of bacteriocins or reduce problems related to their use in biomedical applications. Therefore, bacteriocins combined with nanomaterials have emerged as promising molecules that can be used in various biomedical applications. This review highlights the features of bacteriocins and their limitations in biomedical applications and provides a detailed overview of the uses of different nanomaterials in improving the limitations. Our review focuses on the potential applications of nanomaterials combined with bacteriocins as new designer molecules for use in future therapeutic strategies.

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Section: Different Bacteriocins and Their Spectrummentioning

confidence: 99%

Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins

Naskar

Kim

2021

Pharmaceutics

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“…Nevertheless, until now, bacteriocins application as food preservatives is undergoing various limitations such as degradation by some proteolytic enzymes, limited antimicrobial spectrum, low production and at the same time needing for a high effective dosage [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Bacteriocin-nanoconjugate is considered one of the newest and encouraging solutions which recently developed to overcome some of the aforementioned defects which hinder bacteriocin- industrial applications. Hence, several studies have been evolved for exploring different nanoformulations for bacteriocin [ 12 ]. Bacteriocin incorporated nanoparticles have found to induce several structure changes to bacterial cells that enhancing the interaction with bacterial membrane constituents through disturbing permeability and forming pits, facilitating penetration inside the cell, unbalancing respiratory functions, causing an increase of reactive oxygen species and depletion of ATP levels, intercalating between DNA bases and interfering replication [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of the antibacterial potential of plantaricin by incorporation into silver nanoparticles

Amer¹,

Abushady

Refay³

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Bacteriocins are proteinaceous compounds produced from lactic acid bacteria. Bacteriocins are well-known for their antibacterial potential and safety for application in food. However, the commercial availability of bacteriocin is facing several limitations; among them is the low yield and short stability period. That calls for a new strategy for overcoming these hurdles. Among these approaches is incorporating bacteriocin in nanoparticles. So, the aim of this study was to enhance the plantaricin produced from isolated Lactobacillus plantarum strain using nanotechnology. Results In this study, the plnEF genes encoding plantaricin EF have been identified and sequenced (accession number of MN172264.1). The extracted bacteriocin (EX-PL) was obtained by the ammonium sulfate method. Then, it was used for biosynthesizing plantaricin-incorporated silver nanoparticles (PL-SNPs). The synthesized nanoparticles were confirmed by SEM-EDAX analysis. The antibacterial activity of both combined (PL-SNPs) and extracted plantaricin (EX-PL) were tested against some strains of foodborne pathogenic bacteria. The results revealed that the antibacterial activities were increased by 99.2% on the combination of bacteriocin with the silver nanoparticle. The MIC of EX-PL (7.6 mg/mL) has been lowered after incorporating into silver nanoparticles and reached 0.004 mg/mL for PL-SNPs. Despite that extracted plantaricin showed no inhibitory activity towards Listeria monocytogenes, plantaricin-incorporated silver nanoparticles displayed inhibitory activity against this strain. Furthermore, the stability period at 4 °C was increased from 5 days to 60 days for EX-PL and PL-SNPs, respectively. Conclusions Plantaricin-incorporated silver nanoparticles possess higher antibacterial activity and more stability than the free one, which makes it more fitting for combating foodborne pathogens and open more fields for applications in both food and pharmaceutical industries. Graphical abstract

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“…In this direction, to counter-act this problem, more recently, biopolymer-encapsulated nanoparticles have attracted the interest of researchers. It has been proposed in a few studies that conjugation of nanoparticles with bacteriocins may aid in increasing the antimicrobial activity, enhancing the stability, and also in increasing the shelf life of food [14][15][16]. Metal nanoparticles, because of their properties such as a broad antimicrobial spectrum and a large surface area are known to be promising candidates for conjugation with bacteriocins.…”

Section: Introductionmentioning

confidence: 99%

Bacteriocin‐capped silver nanoparticles for enhanced antimicrobial efficacy against food pathogens

Sidhu

Nehra

2020

IET nanobiotechnol.

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Bacteriocins produced by lactic acid bacteria are safer alternatives to the more popularly used chemical preservatives which exhibit several adverse effects. The bacteriocins have an advantage of being efficient in controlling food pathogens without possessing any side-effects. However, the bacteriocins have a limitation of exhibiting a narrow antimicrobial spectrum and having a high-dosage requirement. With an aim to combat these limitations, the present study involved the biosynthesis of bacteriocin-capped nanoparticles, using two bacteriocins (Bac4463 and Bac22) extracted and purified from Lactobacillus strains. Nanoconjugates synthesised at optimum conditions were characterized using various physico-chemical techniques. The interaction of bacteriocin-capped silver nanoparticles with the pathogenic bacteria was observed using scanning electron microscopy, wherein the deformed and elongated cells were clearly visible. In vitro antimicrobial efficacy of both Bac4463capped silver nanoparticles and Bac22-capped silver nanoparticles against different food pathogens was observed to be enhanced in comparison to the antimicrobial activity of bacteriocins alone. Minimum inhibitory concentration was observed to be as low as 8 μg/ml for Bac4463-capped silver nanoparticles against Staphylococcus aureus, and 2 μg/ml for Bac22-capped silver nanoparticles against Shigella flexneri. This study, therefore, recommends the use of bacteriocin-capped nanoparticles as food preservatives to control the growth of food spoiling bacteria.

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Bacteriocin-nanoconjugates as emerging compounds for enhancing antimicrobial activity of bacteriocins

Cited by 34 publications

References 60 publications

Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins

Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins

Enhancement of the antibacterial potential of plantaricin by incorporation into silver nanoparticles

Bacteriocin‐capped silver nanoparticles for enhanced antimicrobial efficacy against food pathogens

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